Outer membrane protein Q,Helicobacter pylori,Anti-adhesion,Abelmoschus esculentus,Capsicum annum,Curcuma longa," /> Outer membrane protein Q,Helicobacter pylori,Anti-adhesion,Abelmoschus esculentus,Capsicum annum,Curcuma longa,"/> <em>Helicobacter pylori</em> outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents
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Journal of Integrative Medicine ›› 2017, Vol. 15 ›› Issue (5): 398-406.doi: 10.1016/S2095-4964(17)60359-5

• Research Article • Previous Articles     Next Articles

Helicobacter pylori outer membrane protein Q genotypes and their susceptibility to anti-adhesive phytotherapeutic agents

Javed Yakooba,b, Zaigham Abbasa, Malik Hassan Mehmoodb, Kanwal Tariqa, Saima Azhar Saleema, Safia Awana, Abdul Malikb, Saeed Hamida, Rustam Khana, Wasim Jafria   

  1. a Department of Medicine, Aga Khan University, Karachi 74800, Pakistan
    b Department of Biological Biomedical Sciences, Aga Khan University, Karachi 74800, Pakistan
  • Received:2017-02-15 Accepted:2017-05-24 Online:2017-09-15 Published:2017-09-15
  • Contact: Javed Yakoob; E-mail: javed.yakoob@aku.edu


Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q (HopQ) mediates host-pathogen interactions; HopQ genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against HopQ genotypes and H. pylori cytotoxin-associated gene A (CagA).


DNA was extracted by polymerase chain reaction of the HopQ genotypes (i.e., type 1, type 2 and CagA) from 115 H. pylori strains. The effect of the extracts from selected dietary ingredients was determined using a gastric adenocarcinoma cell line and a quantitative DNA fragmentation assay. The anti-adhesive effect of these extracts on H. pylori was tested using an anti-adhesion analysis.


C. annum, C. longa and A. esculentus showed prominent anti-adhesion effects with resultant values of 17.3% ± 2.9%, 14.6% ± 3.7%, 13.8% ± 3.6%, respectively, against HopQ type 1 and 13.1% ± 1.7%, 12.1% ± 2%, 11.1% ± 1.6%, respectively, against HopQ type 2. C. longa (93%), C. annum (89%) and A. esculentus (75%) had better anti-adhesive activity against H. pylori with HopQ type 1 compared to HopQ type 2 with respective values of 70%, 64% and 51%. Extracts of C. annum (14.7% ± 4.1%), A. esculentus (12.3% ± 4.1%) and Z. officinale (8.4% ± 2.8%) had an anti-adhesion effect against CagA-positive H. pylori strains compared to CagA-negative strains.


The anti-adhesion properties of the tested phytotherapeutic dietary ingredients were varied with HopQ genotypes. HopQ type 1 was found to be more sensitive to extracts of C. annum, C. longa and A. esculentus compared to the HopQ type 2 genotype.

Key words: Outer membrane protein Q, Helicobacter pylori, Anti-adhesion, Abelmoschus esculentus, Capsicum annum, Curcuma longa

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