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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 508-511.doi: 10.3736/jcim20080515

• Original Experimental Research • Previous Articles     Next Articles

Wenyang Huoxue Recipe up-regulates the expression of angiopoietin-1 mRNA in rats with chronic aristolochic acid nephropathy

Hong-ting Wang, Yun-man Wang, Yong-ping Liang, Yu-jun Liu, Wen Peng()   

  1. Department of Nephrology, Putuo District Center Hospital, Shanghai 200062, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15
  • Contact: PENG Wen E-mail:wenpeng1968@yahoo.com.cn

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Objective: To investigate the effects of Wenyang Huoxue Recipe (WRHXR), a compound traditional Chinese herbal medicine for warming yang and promoting blood flow, on the expression of angiopoietin mRNA in rats with chronic aristolochic acid nephropathy induced by Caulis Aristolochia Manshuriensis (CAM) decoction, and to explore the protection mechanism of WYHXR against kidney damage.
Methods: Twenty-eight male SD rats were randomly divided into normal control group, CAM group and WYHXR-treated group. Rats in the normal control group (n=8) and CAM group (n=10) were intragastrically administered with normal saline 10 ml/(kg·d) or CAM decoction 10 ml/(kg·d) respectively. Rats in the WYHXR-treated group (n=10) were intragastrically administered with WYHXR 30 g/(kg·d) and CAM decoction 10 ml/(kg·d). The expressions of Ang-l and Ang-2 mRNAs were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) after 20-week treatment.
Results: Compared with the normal control group, the expression of Ang-l mRNA was significantly decreased, and the expression of Ang-2 mRNA was significantly increased in the CAM group (P<0.01). Compared with the CAM group, the expression of Ang-l mRNA was increased in the WYHXR-treated group (P<0.01). The expression of Ang-2 mRNA had no significant difference between the CAM group and the WYHXR-treated group (P>0.05). Renal pathology showed that renal damage in WYHXR-treated group was significantly reduced as compared with the CAM group.
Conclusion: WYHXR can up-regulate the expression of Ang-l mRNA, which may be its action mechanism in protecting the kidneys.

Key words: aristolochic acid, Wenyang Huoxue Recipe, angiopoietins, Caulis Aristolochia Manshuriensis, rats, nephropathy

CLC Number: 

  • R289.5

Figure 1

Pathological section of renal tissue in rats in three groups (HE staining, ×400) A: Normal control group; B: CAM group; C: WYHXR-treated group."

Figure 2

Pathological section of renal tissue in rats in three groups (Masson staining, ×400) A: Normal control group; B: CAM group; C: WYHXR-treated group."

Table 1

Expressions of Ang-1 and Ang-2 mRNAs in renal tissues of rats in three groups ($\bar{x}$±s)"

Group n Ang-l mRNA Ang-2 mRNA
Normal control 7 0.008 9±0.003 7 0.002 5±0.001 9
CAM 8 0.001 4±0.000 4** 0.007 6±0.003 1**
WYHXR-treated 8 0.004 1±0.001 1**△△ 0.006 5±0.002 6**
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[1] Hui-ling Wang, Jing-yuan Zhang. Aristolochic acid induces renal tubular injury and inhibits expression of bone morphogenetic protein-7 mRNA in renal tissue of rats. Journal of Chinese Integrative Medicine, 2008, 6(5): 502-507.
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