SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 512-516.doi: 10.3736/jcim20080516

• Original Experimental Research • Previous Articles     Next Articles

Effects of Dahuang Zhechong Pill on expressions of tissue inhibitor of metalloproteinase-1 and plasminogen activator inhibitor-1 in rats with glomerulosclerosis

Ji-hong Chen1, Wei Sun1, Dong Zhou1, Kun Gao1, Wei-ming He1, Li Liu2   

  1. 1. Department of Nephrology, National Medical Centre for Traditional Chinese Medicine Nephropathy, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
    2. Molecular Laboratory, Department of Nephrology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15

RichHTML

1

PDF (PC)

72

Like

Objective: To explore the mechanisms of the beneficial effects of Dahuang Zhechong Pill (DHZCP), a Chinese patent herbal medicine, in treatment of chronic renal disease, and to investigate the effects of DHZCP on the expressions of renal tissue inhibitor of metalloproteinase-1 (TIMP-1) and plasminogen activator inhibitor-1 (PAI-1) mRNAs in rats with adriamycin-induced glomerulosclerosis.
Methods: Focal segmental glomerulosclerosis and diffuse mesangial proliferation were induced in rats by combined procedures, including unilateral nephrectomy, intravenous injection of adriamycin and giving high-fat foods. The rats were randomly divided into untreated group, benazepril-treated group and DHZCP-treated group. Another 6 rats were sham-operated as control group. After 12-week treatment, rats were sacrificed, reverse transcription polymerase chain reaction (RT-PCR) method and computerized image analytical technique were used to determine the expressions of TIMP-1 and PAI-1 mRNAs.
Results: Compared with the untreated group, the ratios of TIMP-1/β-actin and PAI-1/β-actin of the DHZCP-treated group were decreased, suggesting that DHZCP could down-regulate the expressions of TIMP-1 and PAI-1 mRNAs (P<0.05). Benazepril could significantly inhibit the expression of TIMP-1 mRNA compared with that of the untreated group (P<0.05).
Conclusion: DHZCP can down-regulate the expressions of TIMP-1 and PAI-1 mRNAs in renal tissues of rats with focal segmental glomerulosclerosis and diffuse mesangial proliferation, which may be its action mechanism in treating chronic renal disease.

Key words: Dahuang Zhechong Pill, glomerulosclerosis, focal segmental, tissue inhibitor of metalloproteinase- 1, plasminogen activator inhibitor-1, rats

CLC Number: 

  • R289.5

Figure 1

Pathological characteristics of renal tissue in rats in different groups (HE staining, ×200) A: Sham-operated group; B: Untreated group; C: Benazepril-treated group; D: DHZCP-treated group."

Figure 2

Expression of TIMP-1 mRNA in renal tissue in rats in different groups A: Sham-operated group; B: Untreated group; C: Benazepril-treated group; D: DHZCP-treated group."

Figure 3

Expression of PAI-1 mRNA in renal tissue in rats in different groups A: Sham-operated group; B: Untreated group; C: Benazepril-treated group; D: DHZCP-treated group."

[1] Balbín M, Fueyo A , Knuper V, et al.Collagenase 2(MMP-8) expression in murine tissue-remodeling processes. Analysis of its potential role in postpartum involution of the uterus[J]. J Biol Chem, 1998,273(37):23959-23968
doi: 10.1074/jbc.273.37.23959
[2] Kleiner DE Jr, Stetler-Stevenson WG . Structural biochemistry and activation of matrix metalloproteases[J]. Curr Opin Cell Biol, 1993,5(5):891-897
doi: 10.1016/0955-0674(93)90040-W pmid: 8240832
[3] Johnson R, Yamabe H, Chen YP , et al. Glomerular epithelial cells secrete a glomerular basement membrane-degrading metalloproteinase[J]. J Am Soc Nephrol, 1992,2(9):1388-1397
doi: 10.1111/j.1464-410X.1992.tb15548.x pmid: 1627762
[4] Martin J, Knowlden J, Davies M , et al. Identification and independent regulation of human mesangial cell metalloproteinases[J]. Kidney Int, 1994,46(3):877-885
doi: 10.1038/ki.1994.345 pmid: 7996810
[5] Wang JZ, Chen XM, Shi SZ , et al. Expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in IgA nephropathy[J]. Zhonghua Nei Ke Za Zhi, 2002,41(2):75-78
[6] Baricos WH , Cortez SL, el-Dahr SS, et al.ECM degradation by cultured human mesangial cells is mediated by a PA/plasmin/MMP-2 cascade[J]. Kidney Int, 1995,47(4):1039-1047
doi: 10.1038/ki.1995.150 pmid: 7540230
[7] Lin HL, Yu ZH, Fu B , et al. Effect of TIMP-1 on expression of FN and type Ⅳ collagen in rat glomerular mesangial cells[J]. Zhonghua Shen Zang Bing Za Zhi, 2002,18(3):166-170
林洪丽, 于志恒, 傅博 , 等. 金属蛋白酶组织抑制剂-1基因转染对系膜细胞表达纤连蛋白和Ⅳ型胶原的影响[J]. 中华肾脏病杂志, 2002,18(3):166-170
[8] Oda T, Jung YO, Kim HS , et al. PAI-1 deficiency attenuates the fibrogenic response to ureteral obstruction[J]. Kidney Int, 2001,60(2):587-596
doi: 10.1046/j.1523-1755.2001.030002587.x pmid: 1147364122
[9] Ramos-DeSimone N, Hahn-Dantona E, Sipley J , et al. Activation of matrix metalloproteinase-9(MMP-9) via a converging plasmin/stromelysin-1 cascade enhances tumor cell invasion[J]. J Biol Chem, 1999,274(19):13066-13076
doi: 10.1074/jbc.274.19.13066
[10] Oh CK, Ariue B, Alban RF , et al. PAI-1 promotes extracellular matrix deposition in the airways of a murine asthma model[J]. Biochem Biophys Res Commun, 2002,294(5):1155-1160
doi: 10.1016/S0006-291X(02)00577-6 pmid: 12074598
[11] Rerolle JP, Hertig A, Nguyen G , et al. Plasminogen activator inhibitor type 1 is a potential target in renal fibrogenesis[J]. Kidney Int, 2000,58(5):1841-1850
doi: 10.1111/j.1523-1755.2000.00355.x pmid: 11044203
[12] Zhang DZ, Wang MN, Zhao DK , et al. The effects of Da Huang Zhe Chong Pill on experimental thrombus formation and plasma TXA2/PGI2 rate[J]. Xi'an Yi Ke Da Xue Xue Bao, 1993,14(4):319-322
张殿增, 王美纳, 赵东科 , 等. 大黄庶虫丸对实验性血栓形成和TXB2/PGI2比值的影响[J]. 西安医科大学学报, 1993,14(4):319-322
[13] Zhang DZ, Wang MN, Qiu PL . Effects of Da Huang Zhe Chong Pill on microcirculation of blood stagnation rats[J]. Xi'an Yi Ke Da Xue Xue Bao, 1994,15(1):37-39, 44
张殿增, 王美纳, 邱培伦 . 大黄庶虫虫丸对血淤证大鼠微循环的影响[J]. 西安医科大学学报, 1994,15(1):37-39, 44
[14] Sun KW, Chen X, Liu WS , et al. Experimental study of Dahuangzhechong Pill in treatment of immunological liver fibrosis in rats[J]. Zhongguo Zhong Yao Za Zhi, 1998,23(8):497-499
孙克伟, 陈翔, 刘伟士 , 等. 大黄庶虫虫丸抗大鼠免疫性肝纤维化研究[J]. 中国中药杂志, 1998,23(8):497-499
[1] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3