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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 524-529.doi: 10.3736/jcim20080518

• Original Experimental Research • Previous Articles     Next Articles

Volatile oil of Magnolia biondii inhibits expressions of P-selectin protein in serum and renal tissue of rats with diabetic nephropathy

Xue-peng Sun, Nian-song Wang(), Qin Xue, Feng Wang   

  1. Department of Nephrology, the 6th Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China
  • Received:2008-03-19 Online:2008-05-20 Published:2008-05-15
  • Contact: WANG Nian-song E-mail:wangniansong@yahoo.com.cn

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Objective: To investigate the protection mechanism of volatile oil of Magnolia biondii Pamp. (VOMBP) against diabetic nephropathy in rats by observing its effects on level of soluble P-selectin (sP-selectin) in serum and expression of P-selectin in renal tissue.
Methods: Fifty SD rats were randomly divided into normal control group, untreated group, and low-, medium- and high-dose VOMBP-treated group. Diabetic nephropathy was induced in rats by intraperitoneal injection of 1% streptozotocin. Before and the 1st day, 4th, 8th and 12th week after the induction, random blood glucose (RBG) and 24-hour urinary micro-albumin were detected in different groups. At the 12th week, the rats were sacrificed to collect the blood samples and renal tissues. The contents of blood urea nitrogen (BUN) and serum creatinine (SCr) were detected and the pathological change in renal tissues was observed by light microscope; the level of sP-selectin was detected by enzyme-linked immunosorbent assay and the expression of P-selectin protein in renal tissues was measured by immunohistochemical method.
Results: Compared with the normal control group, RBG, 24-hour urinary micro-albumin, the contents of BUN, sP-selectin in serum and expression of P-selectin protein in renal tissue in the untreated group were significantly increased (P<0.01), the content of SCr was significantly decreased (P<0.01), and pathological change of renal tissues was also obvious. Compared with the untreated group, 24-hour urinary micro-albumin, the level of sP-selectin in serum and expression P-selectin protein in renal tissue of the three VOMBP-treated groups were all significantly decreased (P<0.01), and pathological change was lessened too. However, there were no significant differences among the three VOMBP-treated groups (P>0.05).
Conclusion: VOMBP can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue.

Key words: diabetic nephropathy, P-selectin, Magnolia biondii Pamp, oils, volatile, rats, diabetes mellitus

CLC Number: 

  • R587.1

Table 1

Random blood glucose of rats in different groups （$\bar{x}$±s, mmol/L）"

Group n Before model
establishing		 After model established
1st day 4th week 8th week 12th week
Normal control 10 6.80±0.48 6.70±0.36 6.14±0.68 6.38±0.43 5.60±0.28
Untreated 10 7.03±0.54 22.64±1.71** 31.15±0.54** 29.41±4.6** 32.00±0.12**
Low-dose VOMBP-treated 10 6.38±0.68 24.47±2.09** 30.23±3.39 ** 30.83±3.21** 30.67±1.86**
Medium-dose VOMBP -treated 10 6.48±0.49 23.15±1.62** 30.75±2.90** 29.67±4.71** 27.08±5.70**
High-dose VOMBP -treated 10 6.72±0.41 25.00±2.33** 30.96±2.70** 29.46±2.79** 29.85±2.97**

Table 2

24-hour urinary micro-albumin of rats in different groups （$\bar{x}$±s, mg/24 h）"

Group n Before model
establishing		 After model established
1st day 4th week 8th week 12th week
Normal control 10 0.065±0.034 0.070±0.030 0.068±0.036 0.057±0.029 0.052±0.024
Untreated 10 0.067±0.042 0.238±0.048** 0.451±0.279** 0.664±0.424** 1.305±0.305**
Low-dose VOMBP-treated 10 0.096±0.013 0.275±0.049** 0.311±0.058** 0.599±0.286** 0.373±0.057**△△
Medium-dose VOMBP -treated 10 0.087±0.009 0.276±0.063** 0.463±0.324** 0.463±0.307** 0.461±0.254**△△
High-dose VOMBP -treated 10 0.081±0.010 0.274±0.049** 0.356±0.133** 0.590±0.299** 0.538±0.223**△△

Table 3

Contents of BUN and SCr in rats in different groups at the 12th week （$\bar{x}$±s）"

Group n BUN (mmol/L) SCr (μmol/L)
Normal control 10 6.31±0.98 40.07±7.11
Untreated 10 12.29±4.83** 27.11±7.69**
Low-dose VOMBP-treated 10 10.17±2.17 ** 26.33±7.77 **
Medium-dose VOMBP-treated 10 9.96±1.01 ** 23.60±3.91**
High-dose VOMBP-treated 10 10.94±2.22** 27.50±6.48 **

Figure 1

Results of pathological section of kidney in rats in different groups (PAS staining, ×200） A: Normal control group; B: Untreated group; C: Low-dose VOMBP-treated group; D: Medium-dose VOMBP-treated group; E: High-dose VOMBP-treated group."

Table 4

Talbe 4 Content of sP-selectin in serum and expressin of P-selectin protein in renal tissue of rats in different groups at the 12th week （$\bar{x}$±s）"

Group n sP-selectin (ng/L) P-selectin protein expression
Normal control 10 46.41±10.04 0.104±0.026
Untreated 10 82.50±24.37 ** 0.214±0.030**
Low-dose VOMBP-treated 10 52.88±26.35▲▲ 0.117±0.017△△
Medium-dose VOMBP-treated 10 22.72±14.16**△△ 0.119±0.021△△
High-dose VOMBP-treated 10 46.29±25.98△△ 0.133±0.030△△

Figure 2

Expression of P-selectin protein in renal tissue of rats in different groups (Immunohistochemical staining, ×200） A: Normal control group; B: Untreated group; C: Low-dose VOMBP-treated group; D: Medium-dose VOMBP-treated group; E: High-dose VOMBP-treated group."

[1] Nauyen D, Ping F, Mu W , et al. Macrophage accumulation in human progressive diabetic nephropathy[J]. Nephrology (Carlton), 2006,11(3):226-231
doi: 10.1111/j.1440-1797.2006.00576.x pmid: 16756636
[2] Mou X, Jiang M, Song ML , et al. Experience introduction of Professor Jin-xi Zhao in treating diabetic nephropathy[J]. Xin Zhong Yi, 2005,37(11):15-16
牟新, 姜淼, 宋美铃 , 等. 赵进喜教授治疗糖尿病肾病经验介绍[J]. 新中医, 2005,37(11):15-16
[3] Wang XW, Nan HM . Experience introduction of Professor Zheng Nan in treating diabetic nephropathy[J]. Xin Zhong Yi, 2005,37(5):14-15
doi: 10.3969/j.issn.0256-7415.2005.05.006
王雪威, 南红梅 . 南征教授治疗糖尿病肾病经验介绍[J]. Xin Zhong Yi, 2005,37(5):14-15
doi: 10.3969/j.issn.0256-7415.2005.05.006
[4] Li Y, Zhao Y, Huang QF , et al. Study on mechanism of Luo disease on diabetic nephropathy[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2002,8(3):68-70
李岩, 赵雁, 黄启福 , 等. 糖尿病肾病络病机制研究[J]. 中国中医基础医学杂志, 2002,8(3):68-70
[5] Su BY, Li LW . My opinion on diabetic nephropathy therapy based on network vessel theory[J]. Zhejiang Zhong Yi Za Zhi, 2006,41(7):383-384
doi: 10.3969/j.issn.0411-8421.2006.07.007
苏宝印, 李立伟 . 运用络病理论治疗糖尿病肾病之我见[J]. 浙江中医杂志, 2006,41(7):383-384
doi: 10.3969/j.issn.0411-8421.2006.07.007
[6] Wang WK, Shen YJ, Qi Y , et al. Anti-inflammatory mechanism of the volatile oil of Magnolia biondii Pamp[J]. Zhongguo Shou Yi Xue Bao, 2006,25(3):301-303
doi: 10.3969/j.issn.1005-4545.2005.03.024
王文魁, 沈映君, 齐云 , 等. 望春花油的抗炎机理[J]. 中国兽医学报, 2006,25(3):301-303
doi: 10.3969/j.issn.1005-4545.2005.03.024
[7] Xiong TQ, Hu P, Qin XH , et al. Effect of biond magnolia flower on adhesion of eosinophile granulocytes[J]. Zhong Yi Za Zhi, 2006,47(4):306
doi: 10.3321/j.issn:1001-1668.2006.04.044
熊天琴, 胡鹏, 秦旭华 , 等. 辛夷对嗜酸性粒细胞黏附的影响[J]. 中医杂志, 2006,47(4):306
doi: 10.3321/j.issn:1001-1668.2006.04.044
[8] 陈志东, 王锋, 汪年松 , 等. 辛夷挥发油对内皮细胞与中性粒细胞黏附的抑制作用[J]. 陕西中医, 2005,26(10):1119-1120
Chen ZD, Wang F, Wang NS , et al. Inhibiting effect of volatile oil of biond magnolia flower on adhesion of endotheliocytes and neutrophils[J]. Shannxi Zhong Yi, 2005,26(10):1119-1120
[9] Eschen O, Christensen JH, Schmidt EB . Soluble cellular adhesion molecules in patients with diabetes mellitus: relation to microvascular complications[J]. Endocrinologis, 2006,16(6):303-307
doi: 10.1097/01.ten.0000249139.45366.36
[10] Iwamoto M, Mizuiri S, Arita M , et al. Nuclear factor-kappaB activation in diabetic rat kidney: evidence for involvement of P-selectin in diabetic nephropathy[J]. Tohoku J Exp Med, 2005,206(2):163-171
doi: 10.1620/tjem.206.163
[11] Liu Y, Burdon KP, Langefeld CD , et al. P-selectin gene haplotype associations with albuminuria in the Diabetes Heart Study[J]. Kidney Int, 2005,68(2):741-746
doi: 10.1111/j.1523-1755.2005.00452.x pmid: 16014051
[12] Wu YL. Study of luo disease[M]. Beijing: Chinese Science and Technology Publishing House,2004:98- 100, 160
吴以岭 . 络病学[M]. 北京: 中国科学技术出版社,2004:98- 100, 160
[13] Ahn KS, Jung KY, Kim JH , et al. Inhibitory activity of lignan components from the flower buds of Magnoliae fargesii on the expression of cell adhesion molecules[J]. Biol Pharm Bull, 2001,24(9):1085-1087
doi: 10.1248/bpb.24.1085
[14] Shen YJ. Study on Chinese diaphoresis relieving superficies medicine[M]. Beijing: Chinese Medicine Technology Publishing House, 2004: 266-279
沈映君 . 中药解表方药研究[M]. 北京: 中国医药科技出版社, 2004: 266-279
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