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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (12): 1150-.doi: 10.3736/jcim20091211

• Original Experimental Research • Previous Articles     Next Articles

Effects of oral and external application of recipes for promoting granulation in rats with chronic skin ulcers

Liang Shen , Hua-Fa Que   

  1. Department of Traditional Chinese Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-09-15 Accepted:2009-10-28 Online:2009-12-20 Published:2009-12-15
  • Contact: Hua-Fa Que E-mail:huafaque@126.com

Objective

To investigate the efficacy of oral, external and oral plus external application of recipes for promoting granulation on chronic skin ulcers in rats.
Methods

Sixty SD male rats were randomly divided into normal group, untreated group, oral group, external group and combined group (n=12). Chronic skin ulcer was induced by hydrocortisone injection and back skin incision. After oral administration of Fuzheng Huyu Recipe or (and) external application of Baiyu ointment, the compound traditional Chinese medicines for wound healing, healing time and healing rate of the ulcers were observed.
Results

The healing rate was lower and the healing time was longer in the untreated group than in the normal group (P<0.01). The healing rates of the oral group and the combined group were higher than those of the untreated group at all observation points (P<0.05), and the healing rate of the external group was higher on the 3rd and 14th day after model establishment (P<0.05). On the 7th and 10th day after model establishment, the healing rates of the oral group and the combined group were higher than those of the external group (P<0.05), and the healing time of the combined group was shorter than that of the oral group and external group (P<0.05).
Conclusion

Both orally and externally administered wound healing recipes can promote the restoration of chronic refractory ulcers; the effect of combined administration is superior to single administration. Oral, external and combined application can all improve the wound healing in inflammatory phase. Oral and combined application can obviously improve the wound healing in granulation phase and epithelial phase.

Table 1

Wound healing rate ($\bar{x}$±s, %)"

Group n Wound healing rate
3rd day 7th day 10th day 14th day
Normal 12 21.35±8.61 61.32±9.03 83.05±2.86 94.94±2.75
Untreated 12 20.06±6.69 46.41±8.05** 72.77±2.69** 89.53±5.23**
Oral 11 36.28±4.69**△△ 59.76±5.58△△▲ 80.05±5.25△△▲▲ 95.50±3.40△△
External 9 34.17±12.91**△△ 51.33±14.47* 72.63±10.14* 95.58±5.47△△
Combined 12 36.13±6.07**△△ 59.65±6.75△△▲ 78.75±6.74△▲ 96.38±2.21△△

Table 2

Wound healing time ($\bar{x}$±s, days)"

Group n Wound healing time
Normal 12 15.50±1.17
Untreated 12 19.58±2.64**
Oral 11 17.36±2.42*△
External 9 18.00±2.12**
Combined 12 16.09±1.51△△▲□
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