Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (12): 1154-1158.doi: 10.3736/jcim20091212

• Original Experimental Research • Previous Articles     Next Articles

Effects of mild moxibustion on angiogenesis and microcirculation in wound repair after operation of anal fistula in rats

 Xiu-tian Guo),Qing-jun Dong, Yong-qing Cao   

  1. Department of Anorectal Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-09-02 Accepted:2009-10-31 Online:2009-12-20 Published:2009-12-15
  • Contact: Xiu-tian Guo E-mail:guoxiutian@126.com

Objective

To observe the effects of mild moxibustion on angiogenesis and microcirculation in wound repair after operation of anal fistula, and to explore the mechanism of action.
Methods

Seventy-two SD male rats were made to bear an "acute, open, bleeding, infected" wound surface and were divided randomly into mild moxibustion group, microwave group and control group (24 rats per group). The wound surfaces of the treatment groups were treated for 15 min with mild moxibustion or microwave medical instrument from the first day after operation, and the control group was not given any treatment. At the first, third, seventh and fourteenth days after operation (1, 3, 7 and 14 days of treatment), six rats in each group were sacrificed after examining the blood flow of the wound surface, and the samples of granulation tissues were collected. The numbers of CD34 and positive expression of vascular endothelial growth factor (VEGF) in granulation tissues were detected.
Results

In the early and middle stages of wound healing, mild moxibustion could obviously increase the expression of VEGF, the number of CD34, and the blood flow of the wound surface. And the expression of VEGF, the number of CD34, and the blood flow of the wound surface began to increase at the third day, and reached the peak level at the seventh day, and then began to decrease. In the late stage of wound healing (day 14), mild moxibustion could decrease the expression of VEGF, the number of CD34, and decrease the blood flow of the wound surface. There were significant differences between the mild moxibustion group and the control group in the blood flow of the wound surface, the expression of VEGF and the numbers of CD34 in granulation tissues (P <0.05) after treatment, and except the number of CD34 at 14-day treatment, there were also differences between the mild moxibustion group and the microwave group.
Conclusion

Mild moxibustion has better regulative actions on blood flow of the wound surface and the number of CD34 and positive expression of VEGF in granulation tissues, and can regulate microcirculation in wound surface and promote the wound healing.

Key words: Mild moxibustion, Anal fistula, Microcirculation, Vascular endothelial growth factor, CD34, Rats

Table 1

Weight of rats before experiment (?x±s, g)"

Group n Weight
Control 24 201.30±1.89
Microwave 24 198.25±1.89
Mild moxibustion 24 199.70±1.36

Table 2

Blood flow of the wound surface (?x±s, mL/min)"

Group Blood flow of the wound surface
Before treatment (n=24) 1-day treatment (n=24 ) 3-day treatment (n=18) 7-day treatment (n=12) 14-day treatment (n=6)
Control 148.57±1.69 54.42±1.68 84.91±2.21 122.06±3.49 83.60±4.99
Microwave 151.24±2.16 61.61±1.36* 111.42±2.10* 154.56±3.75* 45.10±6.86*
Mild moxibustion 149.15±2.10 63.20±1.67* 134.86±3.10*△ 202.24±2.89*△ 28.63±2.80*△

Table 3

Positive areas of the VEGF expression (?x±s, μm2)"

Group n Positive area of the VEGF expression
1-day treatment 3-day treatment 7-day treatment 14-day treatment
Control 6 4 588.94±222.35 9 575.33±879.66 9 554.17±909.58 14 557.28±779.46
Microwave 6 4 668.67±276.17 12 471.06±886.64* 13 857.39±955.98* 12 575.17±1 098.84
Mild moxibustion 6 4 824.44±321.90 15 666.56±1 020.67*△ 17 196.06±927.93*△ 9 126.06±1 320.66*△

Table 4

OD value of VEGF expression in granulation tissues (?x±s)"

Group n OD value of VEGF
1-day treatment 3-day treatment 7-day treatment 14-day treatment
Control 6 4.89±0.62 10.11±0.55 10.17±0.31 11.94±0.26
Microwave 6 5.50±0.46 12.00±0.26* 11.50±0.28* 11.28±0.53
Mild moxibustion 6 5.67±0.56 12.50±0.28* 12.44±0.21*△ 9.61±0.45*△

Table 5

Numbers of CD34 in granulation tissues (?x±s)"

Group n Numbers of CD34
1-day treatment 3-day treatment 7-day treatment 14-day treatment
Control 6 2.22±0.15 4.39±0.32 5.50±0.69 7.94±1.08
Microwave 6 2.44±0.33 5.83±0.33* 8.78±0.62* 5.11±0.77*
Mild moxibustion 6 2.44±0.21 7.44±0.66*△ 11.67±1.09*△ 3.83±0.77*
[1] Fu XB, Sun TZ, Sheng ZY . Animal models used in research of trauma repair[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 1999,16(5):479-480
doi: 10.3760/j.issn:1001-9030.1999.05.043
付小兵, 孙同柱, 盛志勇 . 几种用于创伤修复研究的动物模型[J]. 中华实验外科杂志, 1999,16(5):479-480
doi: 10.3760/j.issn:1001-9030.1999.05.043
[2] Zheng XP, Xu HQ, Ding YJ, Jin F . Experimental study of Compound Zhuhuang Frost on rabbit back wound contaminated with human feces[J]. Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi, 2002,8(5):361-364
doi: 10.3969/j.issn.1007-6948.2002.05.017
郑雪平, 许慧琴, 丁义江, 金芳 . 复方珠黄霜对兔背部人粪污染创面愈合的实验研究[J]. 中国中西医结合外科杂志, 2002,8(5):361-364
doi: 10.3969/j.issn.1007-6948.2002.05.017
[3] Huang NJ. Anorectal diseases in China[M]. Jinan: Shandong Science and Technology Press, 1996: 729
黄乃健 . 中国肛肠病学[M]. 济南: 山东科学技术出版社, 1996: 729
[4] McCourtney JS, Finlay IG . Setons in the surgical management of fistula in ano[J]. Br J Surg, 1995,82(4):448-452
doi: 10.1002/bjs.1800820406 pmid: 7613885
[5] Ferrara N . Role of vascular endothelial growth factor in the regulation of angiogenesis[J]. Kidney Int, 1999,56(3):794-814
doi: 10.1046/j.1523-1755.1999.00610.x pmid: 10469350
[1] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[2] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[3] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[4] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[5] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[6] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[7] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[8] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[9] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[10] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[11] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[12] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[13] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[14] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
[15] Manjusha Choudhary​, Vipin Kumar​, Surender Singh​. Gastric antisecretory and cytoprotective effects of hydroalcoholic extracts of Plumeria alba Linn. leaves in rats. Journal of Integrative Medicine, 2014, 12(1): 42-51.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[2] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[5] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[6] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[7] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[8] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052
[9] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[10] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096