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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 298-302.doi: 10.3736/jcim20060316

• Original Experimental Research • Previous Articles     Next Articles

Primary study on proteomics about ganoderma lucidium spores promoting survival and axon regeneration of injured spinal motor neurons in rats

Wei Zhang1, Yuan-shan Zeng1, Yang Wang2, Wei Liu2, Jin-jun Cheng1, Sui-jun Chen1   

  1. 1. Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong Province
  • Online:2006-05-31 Published:2006-05-15

Objective

To detect some proteins associated with the effect of ganoderma lucidium spores (GASP) on promoting the survival and axon regeneration of injured spinal motor neurons in rats.

Methods

The rats were divided into normal control group, untreated group and GASP-treated group, and the rats in the last two groups received ventral root avulsion. GASP preparation was fed to the rats in the GASP-treated group for 14 days. The gray matter tissues of the lumbar spinal were sampled from rats in each group after 14 days following ventral root avulsion, and the extracted proteins from these tissues were detected by using 2-dimensional electrophoresis. Matrix assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) was utilized to identify the differentially expressed proteins among these three groups.

Results

There were six kinds of proteins differentially expressed among the three groups, which were collapsin response mediator protein 2 (CRMP-2), F-actin capping protein beta subunit (FCP-β), isocitrate dehydrogenase [NAD] subunit beta (IDH-β), ATPase, glutamate oxaloacetate transaminase-1 (GOT1) and M2 pyruvate kinase (M2-PK). The expression levels of CRMP-2, IDH-β, ATPase and GOT1 were higher in the GASP-treated group than those in the untreated group, while the expression levels of FCP-β and M2-PK were lower than those in the untreated group.

Conclusion

GASP maybe promotes the survival and axon regeneration of injured spinal motor neurons in rats by virtue of up- or down-regulating the expression levels of the proteins mentioned above.

Key words: Ganoderma lucidium spores, Proteomics, Ventral root avulsion, Neuron survival, Axon regeneration, Rats

CLC Number: 

  • R651.21

Figure 1

Two-dimensional electrophoresis maps of proteins among three groups 1-A: Normal control group; 1-B: Untreated group; 1-C: GASP-treated group."

Figure 2

Enlargement of differentially expressed protein spots(shown in the rings) in two-dimensional electrophoresis maps among three groups2-A: Normal control group; 2-B: Untreated group;2-C: GASP-treated group; 2518, 3563, 1739, 2753, 3120, 2621: Serial numbers of protein in NCBInr"

Figure 3

Mass spectrogram of CRMP-2"

Table 1

Results of mass spectroscopy for No. 2518 protein"

Measure mass Computer mass Peptide sequence
consistent with the mass
Coverage Isoelectric point Molecular weight
(kDa)
Protein name
907.499 907.492 VFNLYPR 22.7 6.3 81.34 CRMP-2
1 014.579 1 014.546 SAAEVIAQAR
1 139.636 1 139.602 KPFPDFVYK
1 293.734 1 293.686 MVIPGGIDVHTR
1 322.757 1 322.756 QIGENLIVPGGVK
1 619.826 1 619.786 GLYDGPVCEVSVTPK

Table 2

Differentially expressed proteins among normal control, untreated and GASP-treated groups"

No. Protein name Serial number in NCBInr Coverage Isoelectric point Molecular weight (kDa)
2518 CRMP-2 57105264 22.7 6.3 81.34
3563 FCP-β 1345668 32.5 5.5 31.33
1739 IDH-β 55926203 18.7 9.1 42.34
2753 ATPase 55716047 17.8 5.6 56.53
3120 GOT1 38197390 47.0 6.7 46.41
2621 M2-PK 206205 35.2 7.2 57.76

Figure 4

Comparisons of six kinds of differentially expressed proteins among three groups"

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