大鼠椎间盘软骨终板软骨细胞体外自然退变模型的建立

doi:10.3736/jcim20060315

Abstract

Objective

To set up a natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats in order to offer an appropriate carrier for the study on mechanism of intervertebral disc degeneration.

Methods

The method of enzyme digestion combined with natural subculture was used to set up the in vitro natural degeneration model of chondrocytes derived from the endplate of intervertebral disc of rats. The morphological appearances and microstructures of the chondrocytes of different generations were observed. The expression of collagenⅡ in chondrocytes was detected by immunocytochemical method.

Results

The chondrocytes derived from the endplate of intervertebral disc expressed collagen Ⅱ. After 13 days of culture, the chondrocytes of generation Ⅲ showed that the ability of cell division descended, the nucleoli became unclear, the cells deformed obviously, fusiform shape with weak optical activity appeared, and the intercellular space was enlarged. There were vacuoles and lipid droplets in cytoplasm. The synthesis of collagen Ⅱ, as well as the cell proliferation rate, descended notably. All results showed the natural degeneration process of the chondrocytes.

Conclusion

The in vitro natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats was successfully established. This can offer the cytological basis for study on the mechanism of intervertebral disc degeneration.

Key words: Intervertebral disc, Chondrocyte of the endplate, Degeneration, Animal models

CLC Number:

R556.7

Ji-xiang Shi, Yong-jun Wang, Qi Shi, Quan Zhou, Peng Sun, Qin Bian, Chong-jian Zhou. An in vitro natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats. Journal of Chinese Integrative Medicine, 2006, 4(3): 293-297.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060315

http://www.jcimjournal.com/EN/Y2006/V4/I3/293

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An in vitro natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats

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