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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 681-685.doi: 10.3736/jcim20070616

• Original Experimental Research • Previous Articles     Next Articles

Inhibition effects of compound leech eye drops on apoptosis of lens epithelial cells and expressions of Bcl-2 and Bax genes in rats

Xiu-rong Huang, Ming-xin Qi, Zhao-yang Wang, Yi Chen   

  1. Research Center of Pathophysiology,Fujian College of Traditional Chinese Medicine,Fuzhou,Fujian Province 350003,China
  • Online:2007-11-20 Published:2007-11-15

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Objective: To investigate the inhibition effects of compound leech eye drops (Co-SZ) on apoptosis of lens epithelial cells (LECs) induced by hydrogen peroxide (H2O2) and the expressions of apoptosis-related genes Bcl-2 and Bax in rats.Methods: All fresh transparent LECs in SD rats were bathed in culture medium with H2O2 in vitro, meanwhile Co-SZ were added in the culture medium. All LECs were incubated for 24 hours. The apoptosis rate of LECs was determined by terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling method (TUNEL). The changes of LEC ultrastructure and the formation of apoptotic body were observed by transmission electron microscopy. The expressions of apoptosis-related genes Bcl-2 and Bax were detected by streptavdin-peroxidase-biotin method.Results: The apoptosis rate of LECs in the Co-SZ-treated group was significantly lower than that in the H2O2-treated group. The changes of apoptotic LEC ultrastructure in the Co-SZ-treated group were less than those in the H2O2-treated group. The expression of Bcl-2 protein was up-regulated and the expression of Bax protein was down-regulated in the Co-SZ-treated group as compared with the H2O2-treated group.Conclusion: The LEC apoptosis induced by H2O2 can be inhibited by Co-SZ. The molecular mechanisms of Co-SZ in inhibiting LEC apoptosis may be related to regulating the expressions of apoptosis-related genes Bcl-2 and Bax.

Key words: leeches, epithelial cells, cataract, apoptosis, genes

CLC Number: 

  • R776.1

Figure 1

Apoptosis of lens epithelial cells (TUNEL, ×400)A: Blank control group; B: H2O2-treated group; C: Co-SZ-treated group."

Table 1

Effect of Co-SZ eye drops on apoptosis rate of lens epithelial cells injured by H2O2 ($\bar{x}±S$, %)"

Group n Apoptosis rate t
Blank control 6 3.50±1.84
H2O2-treated 6 92.00±2.55** 68.94
Co-SZ-treated 6 38.50±8.56△△ 14.67

Figure 2

Changes of lens epithelial cell ultrastructure (transmission electron microscopy)"

Figure 3

Morphological changes of expression of apoptosis-related gene Bcl-2 in lens epithelial cells regulated by Co-SZ (SP, ×400)"

Figure 4

Morphological changes of expression of apoptosis-related gene Bax in lens epithelial cells regulated by Co-SZ (SP, ×400)"

Table 2

Effect of Co-SZ on expression of Bcl-2 protein in lens epithelial cells injured by H2O2"

Group n – + ++ +++ ++++ Ridit
Blank control 6 0 0 1 1 4 0.970
H2O2-treated** 6 5 1 0 0 0 0.500
Co-SZ-treated△△ 6 0 0 0 4 2 0.935

Table 3

Effect of Co-SZ on expression of Bax protein in lens epithelial cells injured by H2O2"

Group n – + ++ +++ ++++ Ridit
Blank control 6 4 2 0 0 0 0.000
H2O2-treated* 6 0 0 0 2 4 0.500
Co-SZ-treated△△ 6 3 2 1 0 0 0.055
[1] 祁明信, 黄秀榕, 汪朝阳 , 等. 水蛭滴眼液防治半乳糖白内障的实验研究[J]. 眼科研究, 2001,19(3):220-222
Qi MX, Huang XR, Wang ZY , et al. Experimental study of protection and treatment for galactose cataract by Leech eye drop[J]. Yan Ke Yan Jiu, 2001,19(3):220-222
[2] 祁明信, 黄秀榕, 汪朝阳 , 等. 复方SZ滴眼液对实验性半乳糖白内障的防治作用[J]. 中国病理生理杂志, 2002,18(10):1206-1209
Qi MX, Huang XR, Wang ZY , et al. Protective effect of Co-SZ eye drop on galactose cataract in rats[J]. Zhongguo Bing Li Sheng Li Za Zhi, 2002,18(10):1206-1209
[3] 黄秀榕, 祁明信, 张松青 , 等. 用晶体混浊分类系统Ⅲ探讨复方水蛭滴眼液防治老年性白内障的临床研究[J]. 中国临床药理学与治疗学, 2004,9(1):63-66
Huang XR, Qi MX, Zhang SQ , et al. Clinical study of protection and treatment of leech compound eye drops On senile cataract by the lens opacities classification systemⅢ[J]. Zhongguo Lin Chuang Yao Li Xue Yu Zhi Liao Xue, 2004,9(1):63-66
[4] 曹翠丽, 马常升, 马文岭 , 等. 中药水蛭对缺血/再灌注后脑细胞的抗凋亡作用[J]. 河北医科大学学报, 2000,21(4):193-195
Cao CL, Ma CS, Ma WL , et al. Anti- apoptotic effect of traditional chinese medicin hirudo on cerebral cells after occlusion/reperfusion[J]. Hebei Yi Ke Da Xue Xue Bao, 2000,21(4):193-195
[5] 王洋, 何奇, 王善政 . 水蛭提取液对肺缺血/再灌注后细胞的抗凋亡作用[J]. 山东大学学报(医学版), 2002,40(2):179
Wang Y, He Q, Wang SZ . Anti- apoptotic effect of leech on lung cells after ischemia reperfusion[J]. Shandong Da Xue Xue Bao(Yi Xue Ban), 2002,40(2):179
[6] Hockenbery D, Nunez G, Milliman C , et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death[J]. Nature, 1990,348(6299):334-336
doi: 10.1038/348334a0 pmid: 2250705
[7] Gajewski TF, Thompson CB . Apoptosis meets signal transduction: elimination of a BAD Influence[J]. Cell, 1996,87(4):589-592
doi: 10.1016/S0092-8674(00)81377-X pmid: 8929527
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