SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 193-195.doi: 10.3736/jcim20040312

• Original Experimental Research • Previous Articles     Next Articles

Effects of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes

Shu-hai Wang1(), Wen-jian Wang1, Xue-feng Wang2, Wei-hua Chen1   

  1. 1. Institute of Integrated Traditional Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
    2. Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Online:2004-06-20 Published:2018-10-25

RichHTML

3

PDF (PC)

347

Like

Objective: To observe the effects of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes. Methods: 3H-glucose uptaking rate in adipocytes treated by salidroside was detected. Adipocytes treated by salidroside in differentiation were stained by oil red O and analyzed by spectrophotography quantitatively. The expression of PPAR-γ and C/EBP-α mRNA relating to differentiation of adipocytes was detected by reverse transcription PCR. Results: 3H-glucose uptaking rate in the salidroside group was 110.4％, higher than that in the blank control group (P<0.01). Salidroside suppressed the differentiation of 3T3-L1 pre-adipocytes and down-regulated the expression of PPAR-γ and C/EBP-α mRNA (P<0.01, vs control group). Conclusion: Salidroside promotes the 3H-glucose uptaking, suppresses the differentiation and down-regulates the expression of PPAR-γ and C/EBP-α mRNA in 3T3-L1 adipocytes.

Key words: salidroside, 3T3 cells, cells differentiation, metabolism, glucose

CLC Number: 

  • R285

Figure 1

3H-glucose uptaking rate (n=7)**P<0.01, vs blank control group; △△ P<0.01,vs rosiglitazone treated group"

Tab 1

Differentiation of 3T3-L1 pre-adipocytes (ヌ±S)"

Group n OD
Salidroside treated 12 0.404±0.022**△△
Rosiglitazone treated 12 0.700±0.033**
Blank control 12 0.538±0.053△△

Figure 3

Agarose gel electrophoresis of PPAR-γ1. Salidroside treated; 2. Rosiglitazone treated; 3. Blank control"

Figure 3

Expression of PPAR-γmRNA in 3T3-L1 adipocytes ( n = 6)* * P＜ 0. 01, vs blank control group; △ △ P＜ 0. 01,vs rosiglitazone treated group"

Figure 4

Agarose gel electrophoresis of C/EBP-α1. Salidroside treated; 2. Rosiglitazone treated; 3. Blank control"

Figure 5

Expression of C/EBP-α mRNA in 3T3-L1 adipocytes(n=6)**P<0.01, vs blank control group; △△P<0.01,vs rosiglitazone treated group"

[1] Bloomgarden ZT . Obesity hypertension and insulin resistance[J]. Diabetes Care, 2002,25(11):2088-2097
doi: 10.1007/s00592-005-0175-1 pmid: 20445714
[2] Tamori Y, Masugi J, Nishino N , et al. Role of peroxisome proliferator-activated receptor-γ in maintenance of the characteristics of mature 3T3-L1 adipocytes[J]. Diabetes, 2002,51(7):2045-2055
[3] Takano A, Haruta T, Iwata M , et al. Growth hormone induces cellular insulin resistance by uncoupling phosphatidylinositol 3-kinase and its downstream signals in 3T3-L1 adipocytes[J]. Diabetes, 2001,50(8):1891-1900
[4] Larsen TM, Toubro S, Astrup A . PPAR gamma agonists in the treatment of type II diabetes: is increased fatness commensurate with long-term efficacy[J]. Int J Obes Relat Metab Disord, 2003,27(2):147-161
[5] 王树海, 王文健 . 胰岛素抵抗的发病机制及中西医结合防治策略[J]. 中西医结合学报, 2004,2(1):14-16
Wang SH, Wang WJ . Pathogenesis of insulin resistance and prevention and treatment strategy of integrated traditional Chinese and Western medicine[J]. Zhong Xi Yi Jie He Xue Bao, 2004,2(1):14-16
[6] Rosen ED, Hsu CH, Wang X , et al. C/EBP alpha induces adipogenesis through PPAR gamma: a unified pathway[J]., 2002,16(1):22-26
[7] Ramji DP, Foka P . CCAAT/enhancer-binding proteins: structure, function and regulation[J]. Biochem J, 2002,365(3):561-575
doi: 10.1042/BJ20020508 pmid: 12006103
[8] 赵文, 王庭欣, 蒋东升 , 等. 蔷薇红景天对四氧嘧啶糖尿病小鼠血糖的调节作用[J]. 卫生研究, 1999,28(6):362-363
doi: 10.3969/j.issn.1000-8020.1999.06.024
[9] 段泾云, 张殿增, 范引科 , 等. 复方红景天片药效学研究[J]. 中成药, 1999,21(11):588-591
[10] 徐琪, 郭中钰, 杨洪发 , 等. 红景天对大鼠脑缺血再灌注时自由基损伤保护作用的实验研究[J]. 白求恩医科大学学报, 1999,25(3):232-234
doi: 10.3969/j.issn.1671-587X.1999.03.005
[1] Meng-ya Shan, Ying Dai, Xiao-dan Ren, Jing Zheng, Ke-bin Zhang, Bin Chen, Jun Yan, Zi-hui Xu. Berberine mitigates nonalcoholic hepatic steatosis by downregulating SIRT1-FoxO1-SREBP2 pathway for cholesterol synthesis. Journal of Integrative Medicine, 2021, 19(6): 545-554.
[2] Esmaeil Yousefi Rad, Behzad Nazarian, Somayeh Saboori, Ebrahim Falahi, Azita Hekmatdoost. Effects of L-arginine supplementation on glycemic profile: Evidence from a systematic review and meta-analysis of clinical trials. Journal of Integrative Medicine, 2020, 18(4): 284-291.
[3] Omar Farid, Farid Khallouki, Morad Akdad, Andrea Breuer, Robert Wyn Owen, Mohamed Eddouks. Phytochemical characterization of polyphenolic compounds with HPLC–DAD–ESI–MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats. Journal of Integrative Medicine, 2018, 16(3): 185-191.
[4] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[5] Samadder Asmita, Das Jayeeta, Das Sreemanti, Biswas Raktim, Rahman Khuda-Bukhsh Anisur. Ameliorative potentials of Syzygium jambolanum extract against arsenic-induced stress in L6 cells in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1293-1302.
[6] Anisur Rahman Khuda-Bukhsh, Arnab De, Durba Das, Suman Dutta, Naoual Boujedaini. Analysis of the capability of ultra-highly diluted glucose to increase glucose uptake in arsenite-stressed bacteria Escherichia coli. Journal of Chinese Integrative Medicine, 2011, 9(8): 901-912.
[7] Xiao-ning Wang, Qing Tao, Qin Feng, Jing-hua Peng, Ping Liu, Yi-yang Hu. Effects of Chinese herbal medicine Yiguanjian Decoction on collagen metabolism of hepatic tissues in rats with CCI4-induced liver fibrosis. Journal of Chinese Integrative Medicine, 2011, 9(6): 651-657.
[8] Zhao Li, Liang Xiao-chun, Zhang Hong, Wang Pu-yan, Sun Qing, Huang Wen-zhi, Li Bo-wu. Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium. Journal of Chinese Integrative Medicine, 2011, 9(3): 299-305.
[9] Hong-li Xue, Zeng Zhang, Wen-jian Wang, Wei-hua Chen, Jian Ying. Effects of Chinese herbal medicine Yiqi Zengmin Formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats. Journal of Chinese Integrative Medicine, 2011, 9(10): 1133-1137.
[10] Xiang-yu Guo, Ying Duan, Juan-e Li , Li-xia Yang, Lian-sha Huang, Zhi-cheng Wang, He-yao Wang, Tong-hua Liu. Antidiabetic effects of Tangnaikang on obese Zucker rats and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(6): 535-540.
[11] Xiu-fang Gao, Hai-ming Shi, Tao Sun, Hong Ao. Effects of Radix et Rhizoma Rhodiolae Kirilowii on expressions of von Willebrand factor, hypoxia-inducible factor 1 and vascular endothelial growth factor in myocardium of rats with acute myocardial infarction. Journal of Chinese Integrative Medicine, 2009, 7(5): 434-440.
[12] Li Ma, Dong-lian Cai, Huai-xing Li, Ben-de Tong, Ying Wang, Su-ping Pei. Protective effects of salidroside on oxidative damage in fatigue mice. Journal of Chinese Integrative Medicine, 2009, 7(3): 237-241.
[13] Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.
[14] Gui-song Zhu, Jin-song He. Effects of ligustrazine injection on high glucose-induced type Ⅰ collagen, matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 expressions in human peritoneal mesothelial cells in vitro. Journal of Chinese Integrative Medicine, 2009, 7(1): 65-69.
[15] Li-hui Hou, Xin-ming Yang, Risto Erkkola, Xiao-ke Wu​. Therapeutic effects of Jinqi Jiangtang Tablet on women with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2006, 4(6): 579-584.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3