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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (11): 1041-1047.doi: 10.3736/jcIm20101107

• Original Expreimental Research • Previous Articles     Next Articles

Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs

Wei Xua,b,Meng Shic,Jian-gang Liub, Cheng-long Wangb()   

  1. a Beijing Geriatric Hospital, Beijing 100095, China
    b Department of Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    c Beijing Haidian Health Center, Beijing 100091, China
  • Received:2010-02-23 Accepted:2010-07-13 Online:2010-11-20 Published:2010-11-15
  • Contact: Cheng-long Wang E-mail:wcl207@yahoo.com.cn

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Objective

To investigate collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction (AMI) and the effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs.

Methods

AMI model of Wistar rat was established by ligating left anterior descending coronary artery. The model rats were randomly divided into untreated group, perindopril group (0. 36 mg/kg), Yiqi Yangyin Huoxue (YQYYHX) group (Shengmai Capsule 0. 24 g/kg and Compound Chuanxiong Capsule 0. 40 g/kg), and Jiedu Huoxue (JDHX) group (Compound Chuanxiong Capsule 0.40 g/kg and berberine 0.16 g/kg), with 10 rats in each group. The latter three groups were treated with corresponding drugs by gastric gavage once a day for 4 weeks after modeling. Sham-operated (puncture without ligation) group and normal control group with 10 rats in each group were given normal saline by gastric gavage. Left ventricular mass index (LVMI) was measured, and pathologic changes in myocardium were observed by hematoxylin-eosin staining 4 weeks later. Contents of procollagen type IE and collagen type F in serum were measured by radioimmunoassay, and expressions of collagen types I and IE in ischemic myocardium were measured by immunohistochemical methods.

Results

Compared with rats in the sham-operated group, weight of left ventricle and LVMI of rats in the model group increased significantly; content of procollagen type n in serum increased; contents of collagen types I and IE in ischemic myocardium increased significantly (P < 0. 01). Compared with rats in the untreated group, LVMI and content of procollagen type IE in serum of rats treated with herbs decreased significantly, and expressions of collagen types I and IE in ischemic myocardium were inhibited (P<0.05, P<0.01). The effect of detoxifying and blood-activating herbs on collagen type IE in ischemic myocardium is better than perindopril.

Conclusion

Both qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs have beneficial effects on rats with AMI in decreasing LVMI and procollagen type HI content, and inhibiting expressions of collagen types I and IE in ischemic myocardium, thus protecting ischemic myocardium after AMI. It may explain the machanism of Chinese herbal medicine inhibiting ventricular remodeling after AMI.

Key words: Myocardial infarction, Ventricular remodeling, Type I collagen protein, Type IE collagen protein, Chinese herbal drugs, Rats

Figure 1

Pathological state of myocardium structure of rats with AMI in different groups observed by HE staining (Light microscopy,×400) A: Normal control group; B: Sham-operated group; C: Untreated group; D: Perindopril group; E: YQYYHX group; F: JDHX group."

Table 1

Contents of serum procollagen type Ⅲ and collagen type Ⅳ in rats with AMI in different groups"

Table 2

Left ventricle weight and LVMI of rats with AMI in different groups"

Figure 2

Expression of typeⅠcollagen in myocardial tissue of rats with AMI observed by immunohistochemistical method (Light microscopy,×400) A: Normal control group; B:Shame-operated group; C: Untreated group; D: Perindopril group; E: YQYYHX group; F: JDHX group. Brown color in the picture refers to the expression of typeⅠcollagen protein in myocardium."

Figure 3

Expression of type Ⅲ collagen in myocardial tissue of rats with AMI observed by immunohistochemistical method (Light microscopy,×400) A: Normal control group; B: Sham-operated group; C: Untreated group; D: Perindopril group; E: YQYYHX group; F: JDHX group. Brown color in the picture refers to the expression of type Ⅲ collagen protein in myocardium."

Table 3

Contents of typeⅠand Ⅲ collagen in myocardial tissues in rats with AMI in different groups"

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