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Journal of Integrative Medicine ›› 2014, Vol. 12 ›› Issue (2): 115-120.doi: 10.1016/S2095-4964(14)60011-X

• Research Article • Previous Articles     Next Articles

Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation

Amir Rashidiana, Saeed Mehrzadia, Ali Reza Ghannadib, Parvin Mahzoonic, Samira Sadrd, Mohsen Minaiyane   

  1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
    Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
    Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
    Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  • Received:2013-09-23 Accepted:2014-01-13 Online:2014-03-10 Published:2014-03-15


Ulcerative colitis is a chronically recurrent inflammatory bowel disease of unknown origin. In the present study, the effect of ginger (rhizome of Zingiber officinale Roscoe) volatile oil on a rat model of colitis was evaluated. 


Volatile oil of ginger with doses of 100, 200, and 400 mg/kg, prednisolone (4 mg/kg), or vehicle were administered orally to groups of male Wistar rats (n = 6) for 5 d. Animals were randomly divided into 6 groups, each group consisting of 6 rats. Colitis was induced by intracolonic instillation of 2 mL of 4% (v/v) acetic acid solution. All rats were sacrificed 24 h later and the tissue injuries were assessed macroscopically and histopathologically. 


Ginger volatile oil with all doses reduced colon weight/length ratio (P < 0.01) and the effects were similar to the reference drugs. Higher oral doses of volatile oil (200 and 400 mg/kg) reduced ulcer severity (P < 0.05 and P < 0.01), ulcer area (P < 0.01) and ulcer index (P < 0.01). On the other hand, evaluation of microscopic scores showed that the dose of 400 mg/kg of volatile oil was effective to reduce inflammation severity (P < 0.01) and inflammation extent (P < 0.05) compared to the control group. 


It is concluded that ginger volatile oil could effectively reduce symptoms of experimental colitis in a dose-dependent manner.

Key words: Ginger, Zingiber officinale, Oils, volatile, Acetic acid, Colitis, Rats


Figure 1

Macroscopic presentation of acetic acid-induced colitis in rats A: Colitis in control group treated with vehicle; B: Colitis in prednisolone-treated group; C, D and E: Colitis in ginger volatile oil-treated group, 100, 200 and 400 mg/kg, respectively."

Figure 2

Microscopic presentation of acetic acid-induced colitis in rats stained by haematoxylin and eosin (Light microscopy, ×20) A: Normal colon treated with vehicle, mucus layer and crypts are normal and leucocyte infiltration is absent; B: Colitis in control group treated with vehicle, mucosal and submucusal inflammation as well as crypt damage and leucocyte infiltration are evident (arrows); C: Prednisolone-treated colitis; D: Ginger volatile oil (400 mg/kg)-treated colitis."



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