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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (12): 1427-1432.doi: 10.3736/jcim20121215

• Original Experimental Research • Previous Articles     Next Articles

Effects of echinacoside on MPP+-induced mitochondrial fragmentation, mitophagy and cell apoptosis in SH-SY5Y cells

Min Zhu1,2,3, Mi Zhou3, Ying Shi3, Wen-wei Li 1,2()   

  1. 1. Laboratory of Neurophysiology and Neurophathology, Department of Integrative Medicine,Zhongshan Hospital, Fudan University, Shanghai 200032, China
    2. Institute of Neurology, Fudan University, Shanghai 200040, China
    3. Institute of Brain Science, Fudan University, Shanghai 200032, China
  • Received:2012-08-27 Accepted:2012-10-15 Online:2012-12-20 Published:2018-12-15

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Objective: To observe the protective effect of echinacoside on mitochondrial fragmentation, mitophagy and cell apoptosis in human neuroblastoma SH-SY5Y cell line exposed to 1-methyl-4-phenylpyridinium (MPP +).
Methods: The MPP + -treated SH-SY5Y cells were divided into control and drug groups. The cell apoptosis was determined by flow cytometry analysis. The alterations in mitochondrial morphology were observed, and the changes in mitochondrial membrane potential and mitophagy were measured.
Results: MPP + induced severe mitochondrial fragmentation in SH-SY5Y cells. Mitochondrial membrane potential was significantly reduced (P<0.05), which further led to mitophagy and cell apoptosis in SH-SY5Y cells treated with MPP +(P<0.05); mitochondrial depolarization, mitophagy and cell apoptosis in echinacoside-treated groups were significantly suppressed (P<0.05), but mitochondrial fragmentation was not affected (P>0.05).
Conclusion: MPP + can cause severe damage to mitochondrial morphology and the function in SH-SY5Y cells, and echinacoside selectively reverse mitochondrial function and cell apoptosis, yet there is no effect on mitochondrial morphology.

Key words: 1-methyl-4-phenylpyridinium, Cistanche, neuroblastoma, DNA,mitochondrial, DNA fragmentation, autophagy, apoptosis

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Group n Apoptotic rate at 24 h Apoptotic rate at 36 h
Normal 5 4.3±0.6 5.7±0.8
Model 5 29.3±3.7** 40.8±5.2**
Low-dose echinacoside
(5 μg/L)		 5 15.8±2.3△ 23.3±3.1△
Medium-dose
echinacoside (10 μg/L)		 5 7.5±1.2△▲ 12.9±1.7△▲
High-dose echinacoside
(20 μg/L)		 5 6.9±1.1△▲ 11.7±1.5△▲

Figure 1

Effects of echinacoside on MPP+-induced mitochondrial fragmentation in SH-SY5Y cells Mitochondrial morphology was visualized by laser confocal microscopy in live cell imaging system. A: normal group; B: model group; C: low-dose echinacoside (5 μg/L) group; D: medium-dose echinacoside (10 μg/L) group; E: high-dose echinacoside (20 μg/L) group."

"

Group n MFI of TMRE
Normal 5 70.3±8.9
Model 5 38.6±4.2**
Low-dose echinacoside (5 μg/L) 5 53.1±5.1△
Medium-dose echinacoside (10 μg/L) 5 63.4±5.9△▲
High-dose echinacoside (20 μg/L) 5 64.7±6.4△▲

"

Group n MFI of GFP at 12 h MFI of GFP at 24 h
Normal 5 52.2±6.3 51.9±6.1
Model control 5 41.7±5.8 19.4±3.0**
Low-dose echinacoside
(5 μg/L)		 5 45.9±5.9 33.2±4.3△
Medium-dose echinacoside
(10 μg/L)		 5 48.2±6.1 43.8±5.2△▲
High-dose echinacoside
(20 μg/L)		 5 49.3±5.8 45.6±5.5△▲
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