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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 288-291.doi: 10.3736/jcim20040417

• Original Experimental Research • Previous Articles     Next Articles

An experimental study on distribution of musk into the brain through blood brain barrier

Wen-kai Chen(), Yu-fang Huang, Hai-dong Wang   

  1. Division of Zang-fu Organs’ Research, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Received:2003-09-26 Online:2004-07-20 Published:2018-10-20

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Objective: To study the possible pathway of the effect of musk on brain disorder,distributing into the brain through blood brain barrier.Methods: We used the musk ketone (muscone), a main composition of musk,to inject through the tail vein of the rats into the blood and took the brain and other organs at different times to make samples. Then gas chromatography was used to measure the distribution of muscone in the brain and other organs.Results: Muscone could pass through the normal rat's blood brain barrier into the brain and soon reached the highest peak and remained in higher concentration, and more slowly metabolized as compared with other organs.Conclusion: Musk distributing into the brain through blood brain barrier provides the basis for its effect in treating brain disorders. Chromatography is an effective method to study the active composition of Chinese herbal medicine distributing through the blood brain barrier into the brain.

Key words: musk, muscone, blood brain barrier, chromatography, gas, pharmacokinetics

CLC Number: 

  • R285.1

Fig. 1

Gas chromatogram of standard The first peak is muscone, TR=5.7 min; The second peak is internal standard, TR=7.7 min"

Fig. 2

Gas chromatogram of brain 15 min after iv injection of muscone The first peak is muscone, TR=5.7 min; The second peak is internal standard, TR=7.7 min"

Tab 1

The dynamic changes of muscone in tissues after iv injection ($\bar{x}$±s , μg/g )"

Time n Brain Heart Spleen Liver Kidney Lung
2 min 6 0.485±0.036 0.404±0.017* * 1.001±0.015* * 3.001±0.203* * 0.751±0.007* * 0.185±0.013 
5 min 6 0.769±0.010 0.461±0.014* * 1.566±0.020* * 3.480±0.108* * 0.770±0.037* * 0.290±0.010* *
15 min 6 0.728±0.012 0.307±0.014* * 0.763±0.021* * 2.879±0.078* * 0.581±0.178* * 0.553±0.010* *
30 min 6 0.661±0.019 0.255±0.015* * 0.610±0.018* * 2.309±0.049* * 0.386±0.008* * 0.263±0.008* *
45 min 6 0.522±0.046 0.192±0.013* * 0.414±0.020* * 1.742±0.050* * 0.285±0.014* * 0.208±0.012* *
60 min 6 0.379±0.008 0.121±0.015* * 0.357±0.014* * 1.050±0.018* * 0.167±0.014* * 0.167±0.010* *
75 min 6 0.335±0.013 0.088±0.045 0.273±0.045* * 0.839±0.068* * 0.142±0.012* * 0.140±0.010* *
90 min 6 0.274±0.020 0.066±0.007 0.234±0.017* * 0.696±0.022* * 0.084±0.013* * 0.083±0.006* *

Fig. 3

Decline of muscone in tissues"

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