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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 386-390.doi: 10.3736/jcim20050514

• Original Experimental Research • Previous Articles     Next Articles

Effect of taspine hydrochloride on skin wound healing in rats and its mechanism

Ya-lin Dong1,2,Lang-chong He1,Huai-hui Wang1,Hai-sheng You2,Jiao-feng Wu1   

  1. 1. Department of Pharmacy, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China;
    2. Department of Pharmacy, First Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China;
  • Received:2005-03-17 Online:2005-09-20 Published:2005-09-20
  • Contact: Lang-chong He E-mail:helc@mail.xjtu.edu.cn

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Objective

To study the effect of taspine hydrochloride (TA/HCl) on skin wound healing in rats and its mechanism.

Methods

Bilateral round wounds were made on the backs of SD rats. The effect of TA/HCl on the skin wound was evaluated through determining closure time and contracting ability of the skin wound, observing histopathological characteristics and measuring contents of hydroxyproline (Hyp) and protein in the wound tissue.

Results

The closure time of the skin wounds was significantly shorter in the TA/HCl-treated groups than that in the model group. The percentages of wound contraction were higher in the TA/HCl-treated groups than that in the dimethyl sulfoxide (DMSO) control group of the same group (P<0.05 or P<0.01) on the 3rd to 14th days after wounding. The content of the protein in the wound tissue in the TA/HCl-treated group (2 mg/ml ) was higher than that in the model group (P<0.05) on the 3rd to 7th days after wounding, and it arrived at the peak on the 7th day and gradually decreased to the normal level in skin tissue on the 14th to 21st days after wounding. The contents of Hyp in the wound tissues in the TA/HCl-treated groups were higher than that in the model group (P<0.05 or P<0.015) on the 3rd to 21st days after wounding, and they arrived at the peak on the 14th day and at the normal level in skin tissue on the 21st day. Histopathological test results showed that TA/HCl could promote the formation of newly born capillaries in the early period of the wound healing.

Conclusion

TA/HCl has the ability of promoting skin wound healing in rats, and it can also accelerate the growth of newly born capillaries and raise the production of protein and collagen in wound tissue.

Key words: Taspine hydrochloride, Wound healing, Proteins, Hydroxyproline, Capillaries

CLC Number: 

  • R285.5

Fig 1

Closure time of skin wounds in different groups (n=10) *P<0.05, **P<0.01 vs model group"

Tab 1

Contraction rates of skin wound at different time in different groups （$\bar{x}$±s, %）"

Group Precentage of wound contraction
3rd day
(n=25)		 5th day
(n=20)		 7th day
(n=20)		 10th day
(n=15)		 14th day
(n=15)		 18th day
(n=10)
Model
DMSO control 35.8±6.7 41.7±5.2 60.5±9.2 84.0±3.4 97.5±1.1 99.7±0.2
Blank control 30.4±12.6 33.6±12.6 52.2±13.7 76.8±12.1 95.1±3.5 99.2±1.2
bFGF-treated
DMSO control 38.4±11.5 45.2±10.7 57.8±12.9 79.9±8.5 95.7±2.8 99.1±0.9
bFGF 48.5±6.7** 55.8±7.1** 67.6±8.8* 87.7±6.6* 98.0±1.8* 99.5±0.6
TA/HCl-treated
DMSO control 37.5±8.5 43.2±5.7 59.0±11.3 80.1±8.3 94.5±5.0 99.4±0.4
TA/HCl 2 mg/ml 44.4±10.0* 49.1±8.8* 67.3±8.5* 88.1±5.0* 98.5±1.6* 99.9±0.3
TA/HCl-treated
DMSO control 40.0±8.5 42.8±5.7 54.9±8.6 80.9±4.9 95.8±2.6 99.3±0.9
TA/HCl 1 mg/ml 43.3±9.2 49.8±7.5** 62.2±9.6* 85.9±3.2* 97.5±2.3 99.6±0.4
TA/HCl-treated
DMSO control 41.3±6.4 45.2±7.0 64.5±6.0 82.2±5.1 93.9±6.0 98.2±2.7
TA/HCl 0.5 mg/ml 45.3±10.0 48.4±10.6 65.4±11.2 85.2±7.2 96.1±4.3 99.0±0.5

Fig 2

Contents of protein in granulation tissues of skin wounds in different healing periods (n=5)"

Fig 3

Contents of Hyp in granulation tissues of skin wounds in different healing periods (n=5) "

Fig 4

Numbers of newly born capillaries in granulation tissues of skin wounds in different healing periods (n=5) "

Fig 5

Histopathological characteristics of granulation tissues of skin wounds on the 7th day after wounding (HE staining, ×200) A: Model group; B: TA/HCl-treated group (2 mg/ml)"

Fig 6

Superstructure of granulation tissues in skin wounds on the 7th day after wounding tested by electron microscope (×5000) A: Model group; B: TA/HCl-treated group (2 mg/ml)"

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