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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 661-664.doi: 10.3736/jcim20070612

• Original Experimental Research • Previous Articles     Next Articles

Effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing

Fu-Lun Li, Bin Li, Zhen-yi Wang, Bin Fan, Wen-bin Xu, Rong Xu   

  1. Department of Dermatology ,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine , of Traditional Chinese Medicine ,Shanghai 200437 ,China Shanghai University
  • Online:2007-11-20 Published:2007-11-15

Objective To study the effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing.Methods: Seventy-two male SD diabetic rats with full-thickness skin lesion were randomly divided into 3 groups: SJHYR 1-treated group, SJHYR 2-treated group and normal saline (NS) control group. SJHYR 1 was prepared with Shengji Recipe (SJR, a compound traditional Chinese herbal medicine for promoting granulation) and Huayu Recipe (HYR, a compound traditional Chinese herbal medicine for resolving stagnation) at a ratio of 1∶2, while SJHYR 2 was prepared with SJR and HYR at a ratio of 1∶1. Immunohistochemical method was used to assess Bax and Bcl-2 protein levels in granulation tissue.Results: SJHYR 1 could accelerate wound healing as compared with SJHYR 2 and NS (P<0.05). On the third day in experiment, Bax and Bcl-2 proteins were not found in any groups, but on the seventh and eleventh day in experiment, Bax and Bcl-2 proteins in SJHYR 1-treated group were much higher than those in the other two groups (P<0.05).Conclusion: SJR and HYR in different ratios may all have a role in regulating Bax and Bcl-2 expression in granulation tissue of diabetic rats during wound healing.

Key words: diabetes mellitus, ulcer, wound healing, Bcl-2 genes, rats

CLC Number: 

  • R587.29
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