蜂毒素对人肝癌细胞线粒体膜蛋白7A6和凋亡相关基因产物Fas及其配体FasL表达的影响

doi:10.3736/jcim20070517

Abstract

Objective: To observe the effects of melittin on the expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and Fas ligand (FasL) in hepatocarcinoma cells in vitro and to study the mechanisms of melittin in inducing apoptosis of hepatocarcinoma cells.Methods: BEL-7402 cell line was treated with melittin in vitro. The expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and FasL were detected by flow cytometry. Fas and FasL mRNAs were analyzed by reverse transcription polymerase chain reaction (RT-PCR) assay.Results: The expression rates of mitochondria membrane protein 7A6 of BEL-7402 hepatocarcinoma cells in 8, 16, 32 μg/ml melittin-treated and control groups were 4.89%, 17.74%, 11.45% and 1.02%, respectively. The expression of Fas protein was up-regulated by melittin, while FasL expression did not change. RT-PCR results showed that Fas mRNA expression was up-regulated by 32 μg/ml melittin and FasL mRNA expression was not observed.Conclusion: The effects of melittin in inducing hepatocarcinoma cell apoptosis may be related with up-regulating the expressions of mitochondria membrane protein 7A6 and Fas protein.

Key words: melittin, liver cancer, mitochondrial proteins, Fas antigens

CLC Number:

R735.7

Chen Zhang, Bai Li, Shu-qin Lu, Yong Li, Yong-hua Su, Chang-quan Ling. Effects of melittin on expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and Fas Ligand in hepatocarcinoma cells. Journal of Chinese Integrative Medicine, 2007, 5(5): 559-563.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070517

http://www.jcimjournal.com/EN/Y2007/V5/I5/559

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References 14

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Group	Fas	β-actin	Ratio
Control	207.67±11.52	176.33±10.59	1.17±0.13
8 μg/ml melittin-treated	172.00±12.42	158.67±10.53	1.10±0.19
16 μg/ml melittin-treated	176.00±12.42	158.10±10.52	1.19±0.17
32 μg/ml melittin-treated	170.67±6.11	147.00±9.53	1.36±0.13*

Effects of melittin on expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and Fas Ligand in hepatocarcinoma cells

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