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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 559-563.doi: 10.3736/jcim20070517

• Original Experimental Research • Previous Articles     Next Articles

Effects of melittin on expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and Fas Ligand in hepatocarcinoma cells

Chen Zhang, Bai Li, Shu-qin Lu, Yong Li, Yong-hua Su, Chang-quan Ling   

  1. Department of T raditional Chinese Medicine ,Changhai Hospital ,Second Military Medical University , Shanghai 200433 ,China
  • Online:2007-09-20 Published:2007-09-15

Objective: To observe the effects of melittin on the expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and Fas ligand (FasL) in hepatocarcinoma cells in vitro and to study the mechanisms of melittin in inducing apoptosis of hepatocarcinoma cells.Methods: BEL-7402 cell line was treated with melittin in vitro. The expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and FasL were detected by flow cytometry. Fas and FasL mRNAs were analyzed by reverse transcription polymerase chain reaction (RT-PCR) assay.Results: The expression rates of mitochondria membrane protein 7A6 of BEL-7402 hepatocarcinoma cells in 8, 16, 32 μg/ml melittin-treated and control groups were 4.89%, 17.74%, 11.45% and 1.02%, respectively. The expression of Fas protein was up-regulated by melittin, while FasL expression did not change. RT-PCR results showed that Fas mRNA expression was up-regulated by 32 μg/ml melittin and FasL mRNA expression was not observed.Conclusion: The effects of melittin in inducing hepatocarcinoma cell apoptosis may be related with up-regulating the expressions of mitochondria membrane protein 7A6 and Fas protein.

Key words: melittin, liver cancer, mitochondrial proteins, Fas antigens

CLC Number: 

  • R735.7

Figure 1

Effect of melittin on expression of 7A6 protein of BEL-7402 cellsA: Isotype; B: Control group; C: 8 μg/ml melittin-treated group; D: 16 μg/ml melittin-treated group; E: 32 μg/ml melittin-treated group."

Figure 2

Effects of melittin on expressions of Fas protein and FasL of BEL-7402 cellsn=4; *P<0.05, **P<0.01, vs control group."

Table 1

Fas mRNA expression in BEL-7402 cells treated with different concentrations of melittin (ヌ±S)"

Group Fas β-actin Ratio
Control 207.67±11.52 176.33±10.59 1.17±0.13
8 μg/ml melittin-treated 172.00±12.42 158.67±10.53 1.10±0.19
16 μg/ml melittin-treated 176.00±12.42 158.10±10.52 1.19±0.17
32 μg/ml melittin-treated 170.67±6.11 147.00±9.53 1.36±0.13*

Figure 3

Electropherogram of Fas mRNAA: β-actin; B: Fas; M: Marker; C: Control group; 1: 8 μg/ml melittin-treated group; 2: 16 μg/ml melittin-treated group; 3: 32 μg/ml melittin-treated group."

Figure 4

Electropherogram of FasL mRNAA: β-actin; B: FasL; M: Marker; C: Control group; 1: 8 μg/ml melittin-treated group; 2: 16 μg/ml melittin-treated group; 3: 32 μg/ml melittin-treated group."

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