[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 56-59.doi: 10.3736/jcim20060115

• Original Experimental Research • Previous Articles     Next Articles

Inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow on cholesterol gallstone formation in guinea pigs

Bang-jiang Fang, Pei-ting Zhu, Jing-zhe Zhang, Xin-jun Pei, Ying-xin Yang, Xiao-qiang Liang   

  1. Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032
  • Online:2006-01-20 Published:2006-01-15

RichHTML

3

PDF (PC)

59

Like

Objective

To explore the mechanism of the inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow (Danshijing Tablets) on cholesterol gallstone formation and provide experimental evidence for its clinical application.

Methods

Eighty guinea pigs were randomly divided into 4 groups, which were normal control group, untreated group, ursodeoxycholic acid (UDCA)-treated group and Danshijing Tablets-treated group. The gallstones in guinea pigs were induced by high-cholesterol diet. UDCA and Danshijing Tablets were given orally to guinea pigs in the corresponding group respectively for seven weeks. Then the physical signs of the guinea pigs, the rates of gallstone formation and the histomorphological changes of the gallbladder were examined. 

Results

The behavior of guinea pigs in the Danshijing Tablets-treated group was obviously improved and the rate of gallstone formation was significantly decreased as compared with those in both untreated and UDCA-treated groups (P＜0.05). The inflammation reaction of mucous membrane in gallbladder was evidently reduced in the Danshijing Tablets-treated group and its morphological appearance turned to be approximately normal.

Conclusion

Recipe for dispersing stagnated liver qi to promote bile flow may decrease gallstone formation. Its mechanism may be related to reducing pathologic changes in gallbladder tissues, which will reduce the damages of cholesterol to the smooth muscle in gallbladder and enhance the contractility of gallbladder.

Key words: Gallstones, Cholesterol, Dispersing stagnated liver qi to promote bile flow

CLC Number: 

  • R575.62

Figure 1

Infrared absorption spectrogram of gallstone in guinea pigs"

Tab 1

Body weight and rate of gallstone formation of guinea pigs after 7-week treatment in 4 groups (ヌ±S)"

GroupnBody weight (g)Number of guinea pigs
with gallstone		Rate of stone formation (%)
Normal control19389.42±39.5915.26
Untreated17276.35±26.67**1482.35**
UDCA-treated18316.22±29.77**△△738.89△
Danshijing Tablets-treated18356.51±30.42**△△▲▲527.78△

Figure 2

Morphological changes of gallbladder tissue in guinea pigs in 4 groups (hematoxylin-eosin staining, ×200)A: Normal control group; B: Untreated group; C: UDCA-treated group; D: Danshijing Tablets-treated group."

[1] 陈希纲, 刘家奇, 彭民浩, 等.胆石病临床流行病学调查——附8585例分析[J].中华普通外科杂志,2002,17(2):99-101
doi: 10.3760/j.issn:1007-631X.2002.02.012
[2] Glass CK, Witztum JL.Atherosclerosis: the road ahead[J].Cell,2001,104(4):503-516
[3] 朱培庭, 张静哲, 曹中平, 等.治疗慢性胆道感染、胆石病274例的总结[J].上海中医药杂志,1986,20(9):15-17
[4] 方邦江, 周爽, 顾宏刚, 等.朱培庭从肝论治胆石病经验[J].中医杂志,2004,45(5):334
[5] 朱培庭, 朱世敏.实用中医胆病学(第1版)[M].北京: 人民卫生出版社,1999,(第1版):272
[6] 陈奇. 中药药理实验方法学(第1版)[M].北京: 人民卫生出版社,1994,(第1版):206
[7] 曾繁清, 牛菲, 李宗山, 等.磁疗排出人体珍珠形胆结石的红外光谱分析[J].中国医学物理学杂志,2000,4(17):245-246
doi: 10.3969/j.issn.1005-202X.2000.04.023
[8] 黄志强. 胆道外科的现状与未来[J].中国实用外科杂志,1999,19(1):17-18
[9] 石景森, 任予.我国胆道外科实验研究的进展[J].中华实验外科杂志,2004,21(12):1418-1419
doi: 10.3760/j.issn:1001-9030.2004.12.002
[10] 沈彬, 吴雪, 王新宇.大黄合剂对急性重症胆管炎大鼠血氧自由基、磷酯酶A2和血浆PGF1α/TXB2的影响[J].中草药,1999,30(10):769-771
[11] 吕慧怡, 王选深.利胆消石胶囊的制备和临床观察[J].中国药业,2001,10(2):30-31
doi: 10.3969/j.issn.1006-4931.2001.02.030
[12] 唐望先, 虞涤霞, 但自力, 等.肝炎平对急性肝损害时脂质过氧化作用的实验研究[J].同济医科大学学报,1998,27(1):56-57
[13] 樊小容. 虎杖对致病菌株药敏试验[J].时珍国医国药,2000,11(2):108
doi: 10.3969/j.issn.1008-0805.2000.02.007
[14] 沈映君. 中药药理学(第1版)[M].上海: 上海科学技术出版社,1997,(第1版):128
[15] 徐彭. 陈皮水提物和陈皮挥发油的药理作用比较[J].江西中医学院学报,1998,10(4):172-173
doi: 10.3969/j.issn.1005-9431.1998.04.022
[16] 宋婷婷, 杨德治.胆囊收缩素对消化功能的影响及其相关机制[J].东南大学学报(医学版),2002,21(2):190-192
doi: 10.3969/j.issn.1671-6264.2002.02.023
[17] 章琳, 董蕾.高胆固醇饮食对胆囊结石形成和胆囊运动的影响[J].西安交通大学学报(医学版),2005,26(3):260-262, 299
[1] Li-li Yang, Miao Wang, Tao Liu, Hai-yan Song, Dong-fei Li, Pei-yong Zheng, Ping Liu, Guang Ji. Effects of Chinese herbal medicine Jiangzhi Granule on expressions of liver X receptor α and sterol regulatory element-binding protein-1c in a rat model of non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2011, 9(9): 998-1004.
[2] Qi-qi Mao , Xu Sun, Dong-ni Qiu, Xiao-dong Fu, Yi Liu, Wen-jian Wang. Effects of Fufang Jiangzhi No.3 on cholesterol-bile acid metabolism in rabbits with hypercholesterolemia. Journal of Chinese Integrative Medicine, 2010, 8(5): 453-457.
[3] Marjan Nassirl-Asl, Farzaneh Zamansoltiani, Esmail Abbasl, Mohammad-Mehdi Daneshi, Amir-Abdollah Zangivand. Effects of Urtica dioica extract on lipid profile in hypercholesterolemic rats. Journal of Chinese Integrative Medicine, 2009, 7(5): 428-433.
[4] Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.
[5] Si-bo Zhang, Bang-jiang Fang. Effects of Yanggan Lidan Granule on rate of gallstone formation and content of plasma cholecystokinin in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2008, 6(4): 405-408.
[6] Xue-lin Zhang, Xiao-qiang Liang, Hong-gang Gu, En-wei Ma, Tian-bi Lin, Xun Sun, Jing-zhe Zhang. Effects of Shengqing Capsule on biochemical parameters in mice with cholesterol gallstone. Journal of Chinese Integrative Medicine, 2008, 6(10): 1045-1048.
[7] Jiong Li, Jing-zhe Zhang, Chang-peng Zou, Bang-jiang Fang, Xiao-qiang Liang. Shengqing Capsule down-regulates estrogen and progesterone receptors in epithelial tissue of gallbladder in guinea pigs with gallstone. Journal of Chinese Integrative Medicine, 2008, 6(10): 1040-1044.
[8] Ping Shen, Bang-jiang Fang, Pei-ting Zhu, Jing-zhe Zhang, Xin-jun Pei. Effect of traditional Chinese herbs for nourishing the liver on intracellular free calcium level in gallbladder cells of guinea pigs with gallstones. Journal of Chinese Integrative Medicine, 2007, 5(2): 179-182.
[9] Guo-ju Dong, Jian-gang Liu, Da-zhuo Shi, Yong-yan Wang, Lian-jun Luan, Yi-yu Cheng. Effects of water extractives of a qi and blood regulating prescription on early atherosclerosis of apolipoprotein E-deficient mice. Journal of Chinese Integrative Medicine, 2007, 5(1): 45-49.
[10] Pei-ting Zhu, Jing-zhe Zhang, Xue-lin Zhang, Zuo-biao Yuan, Hong-ying Zhang, Ju Gao. Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs. Journal of Chinese Integrative Medicine, 2005, 3(3): 207-210.
[11] Si-ying Sun. Experience in treating biliary tract diseases. Journal of Chinese Integrative Medicine, 2003, 1(1): 11-11.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]