复方降脂3号对高胆固醇血症新西兰白兔胆固醇-胆汁酸代谢的影响

doi:10.3736/jcim20100509

Abstract

Objective

To investigate the effects of Fufang Jiangzhi No. 3, a compound traditional Chinese herbal medicine, on cholesterol-bile acid metabolism in rabbits with hypercholesterolemia and to explore the mechanism.
Methods

Twenty-four male New Zealand white rabbits were randomly assigned into normal control group, untreated group and Fufang Jiangzhi No. 3 group, with 8 rabbits in each group. Rabbits in the untreated group and Fufang Jiangzhi No. 3 group were fed high cholesterol diet to induce hypercholesterolemia. After 4-week treatment, serum total cholesterol and bile acid contents were assessed. Activity of cholesterol 7α-hydroxylase (CYP7A1) in liver tissues was measured by enzyme-linked immunosorbent assay. The expressions of CYP7A1, bile salt export pump (BSEP) and small heterodimer partner (SHP) mRNAs in liver tissues were observed by real-time fluorescent quantitative polymerase chain reaction.
Results

Compared with the normal control group, serum total cholesterol and bile acid contents in the untreated group were increased (P<0.01). Activity of CYP7A1 and expression of CYP7A1 mRNA were decreased and expressions of BSEP and SHP mRNAs were increased in liver tissues in the untreated group as compared with the normal control group (P<0.01). Serum total cholesterol level, and expressions of BSEP and SHP mRNAs in the Fufang Jiangzhi No. 3 group were lower than those in the untreated group (P<0.01). The CYP7A1 activity and expression of CYP7A1 mRNA in the Fufang Jiangzhi No. 3 group were increased as compared with the untreated group (P<0.01), however, there was no significant difference in bile acid between the Fufang Jiangzhi No. 3 group and the untreated group.
Conclusion

Fufang Jiangzhi No. 3 can up-regulate the expression of CYP7A1 mRNA, raise the activity of CYP7A1, and inhibit the expressions of BSEP and SHP mRNAs to regulate the metabolism of total cholesterol in rabbits.

Key words: Bile acid, Cholesterol, Chinese medical formula, Farnesoid X receptor, Cholesterol 7-alpha- hydroxylase, Bile salt export pump, Small heterodimer partner receptor, Rabbits

Qi-qi Mao , Xu Sun, Dong-ni Qiu, Xiao-dong Fu, Yi Liu, Wen-jian Wang. Effects of Fufang Jiangzhi No.3 on cholesterol-bile acid metabolism in rabbits with hypercholesterolemia. Journal of Chinese Integrative Medicine, 2010, 8(5): 453-457.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20100509

http://www.jcimjournal.com/EN/Y2010/V8/I5/453

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References 10

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Gene	Primer	Length of product (bp)
SHP	Forward: 5,-TGGCCC A AG AC ATGGTG AC-3, Reverse ： 5 ^GCTCCTCCAGCAGGATCTTCT-3^r	115
BSEP	Forward: 5'-CAACGCATTGCTATTGCTCG-3’ Reverse: 5,-GTTCTGGATGGTGGACAAACG-3,	130
CYP7A1	Forward: 5AGG AG A AGGCG A ATGGGTGC-3, Reverse: 5,-GC AC AGCCC AG AT ATGG A ATC-3,	151
GAPDH	Forward: 5 '-CCG AGGGCCC ACT A A AGG-3 ’ Reverse: 5,-GCTGTTGAAGTCACAGGAGA-3,	166

Group	n	Total cholesterol (mmol/L)	Bile acid (jumol/L)
Normal control	8	4. 51±0. 45	7. 45±1.33
Untreated	8	23. 14±3. 21* *	13. 95±1. 86〃
Compound No. 3	8	16.21±2.14**^AA	13.31±1.27* *

Group	Concentration of n ,	CYP7A1 mRNA
Group	CYP7A1 (pg/mL)	CYP7A1 mRNA
Normal control	8 0. 80±0. 22	1. 97±1. 50
Untreated	8 0. 44±0. 16**	0. 70±0. 27〃
Compound No. 3	8 0. 81±0. 21^AA	3. 87±1. 68**^aa

Group	n SHP mRNA	BSEP mRNA
Normal control	8 9. 83±2. 50	7. 33±2. 44
Untreated	8 85. 44±6. 79〃	16. 40±3. 01〃
Compound No.	3 8 20. 73±4.	9. 64±1. 63**^AA

Effects of Fufang Jiangzhi No.3 on cholesterol-bile acid metabolism in rabbits with hypercholesterolemia

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