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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 56-59.doi: 10.3736/jcim20060115

• Original Experimental Research • Previous Articles     Next Articles

Inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow on cholesterol gallstone formation in guinea pigs

Bang-jiang Fang, Pei-ting Zhu, Jing-zhe Zhang, Xin-jun Pei, Ying-xin Yang, Xiao-qiang Liang   

  1. Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032
  • Online:2006-01-20 Published:2006-01-15


To explore the mechanism of the inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow (Danshijing Tablets) on cholesterol gallstone formation and provide experimental evidence for its clinical application.


Eighty guinea pigs were randomly divided into 4 groups, which were normal control group, untreated group, ursodeoxycholic acid (UDCA)-treated group and Danshijing Tablets-treated group. The gallstones in guinea pigs were induced by high-cholesterol diet. UDCA and Danshijing Tablets were given orally to guinea pigs in the corresponding group respectively for seven weeks. Then the physical signs of the guinea pigs, the rates of gallstone formation and the histomorphological changes of the gallbladder were examined. 


The behavior of guinea pigs in the Danshijing Tablets-treated group was obviously improved and the rate of gallstone formation was significantly decreased as compared with those in both untreated and UDCA-treated groups (P<0.05). The inflammation reaction of mucous membrane in gallbladder was evidently reduced in the Danshijing Tablets-treated group and its morphological appearance turned to be approximately normal.


Recipe for dispersing stagnated liver qi to promote bile flow may decrease gallstone formation. Its mechanism may be related to reducing pathologic changes in gallbladder tissues, which will reduce the damages of cholesterol to the smooth muscle in gallbladder and enhance the contractility of gallbladder.

Key words: Gallstones, Cholesterol, Dispersing stagnated liver qi to promote bile flow

CLC Number: 

  • R575.62

Figure 1

Infrared absorption spectrogram of gallstone in guinea pigs"

Tab 1

Body weight and rate of gallstone formation of guinea pigs after 7-week treatment in 4 groups (ヌ±S)"

GroupnBody weight (g)Number of guinea pigs
with gallstone
Rate of stone formation (%)
Normal control19389.42±39.5915.26
Danshijing Tablets-treated18356.51±30.42**△△▲▲527.78

Figure 2

Morphological changes of gallbladder tissue in guinea pigs in 4 groups (hematoxylin-eosin staining, ×200)A: Normal control group; B: Untreated group; C: UDCA-treated group; D: Danshijing Tablets-treated group."

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