Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1040-1044.doi: 10.3736/jcim20081010

• Original Experimental Research • Previous Articles     Next Articles

Shengqing Capsule down-regulates estrogen and progesterone receptors in epithelial tissue of gallbladder in guinea pigs with gallstone

Jiong Li(), Jing-zhe Zhang, Chang-peng Zou, Bang-jiang Fang, Xiao-qiang Liang   

  1. Department of Hepatobiliary Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-04-10 Online:2008-10-20 Published:2008-10-15

Objective:

To study the role of estrogen receptor (ER) and progesterone receptor (PR) in the formation of cholesterol calculus and investigate the effects of Shengqing Capsule (SQC), a Chinese patent herbal medicine with the function of soothing liver and draining gallbladder, on ER and PR expressions.


Methods:

A total of 80 female guinea pigs were divided into normal control group, untreated group, ursodeoxycholic acid group (UDCA group) and SQC group. The cholesterol gallstone was induced by feeding the guinea pigs with high-fat lithogenic diet. SQC and UDCA were separately administered to the guinea pigs in the SQC group and UDCA group. After 7-week administration, all the animals were sacrificed to calculate the incidence of calculus formation and detect the expressions the ER and PR in the epithelial tissue of gallbladder by immunohistochemical method.


Results: 

Gallstone was cholesterol calculus detected by infrared spectrum. The incidence of calculus formation in the SQC group (27.78%) was significantly lower than that in the untreated group (81.25%) (χ 2=9.721 5, P=0.001 8). On the basis of Reiner standard, the expression distribution of ER and PR increased gradually from the normal control group through the SQC group and UDCA group to the untreated group. Except for the former two groups and the latter two groups, the differences between the other groups and UDCA group were statistically significant (P<0.05). Besides, the differences of positive expression rates between groups were statistically significant (P<0.05).


Conclusion: 

Increased expressions of ER and PR are closely related to the formation of cholesterol stone. And Shengqing Capsule can down-regulate the expressions of ER and PR.

Key words: estrogen receptor, progesterone receptor, cholesterol calculus, guinea pigs, Shengqing Capsule

CLC Number: 

  • R737.9

Table 1

ER expression rate and semi-quantitative grade in epithelial tissue in different groups"

Group n Grade of ER expression ER expression rate (%)
+ ++ +++
Normal control 19 14 3 2 0 26.3
Untreated 16 5 2 4 5 68.8*
UDCA 18 6 2 6 4 66.7*
SQC 18 12 3 2 1 33.3△▲

Figure 1

ER expression in the epithelial tissue of gallbladder of guinea pigs tested by immunohistochemical method (Light microscopy, ×400) A: ER expression (–); B: ER expression (+); C: ER expression (++); D: ER expression (+++)."

Table 2

PR expression rate and semi-quantitative grade in epithelial tissue in different groups"

Group n Grade of PR expression PR expression rate (%)
+ ++ +++
Normal control 19 16 2 1 0 15.8
Untreated 16 6 2 3 5 62.5*
UDCA 18 7 2 4 5 61.1*
SQC 18 11 6 1 0 38.9△▲

Figure 2

PR expression in the epithelial tissue of gallbladder of guinea pigs tested by immunohistochemical method (Light microscopy, ×400) A: PR expression (–); B: PR expression (+); C: PR expression (++); D: PR expression (+++)."

[1] Volzke H, Baumeister SE, Alte D , et al. Independent risk factors for gallstone formation in a region with high cholelithiasis prevalence[J]. Digestion, 2005,71(2):97-105
doi: 10.1159/000084525
[2] Cirillo DJ, Wallace RB, Rodabough RJ , et al. Effect of estrogen therapy on gallbladder disease[J]. JAMA, 2005,293(3):330-339
doi: 10.1001/jama.293.3.330
[3] Zhu PT, Zhu SM. Practice of traditional Chinese medicine for cholic diseases[M]. Beijing: People's Medical Publishing House,1999: 272
朱培庭, 朱世敏 . 实用中医胆病学[M]. 北京: 人民卫生出版社,1999: 272
[4] Reiner G, Neumeister B, Spona J , et al. Immunocytochemical localization of estrogen and progesterone receptor and prognosis in human primary breast cancer[J]. Cancer Res, 1990,50(21):7057-7061
[5] Sun BJ, Cui NQ, Zhang XB , et al. Clinical analysis of 18 079 cases of chololithiasis: a single institution's study[J]. Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi, 2006,12(4):324-327
doi: 10.3969/j.issn.1007-6948.2006.04.003
孙百军, 崔乃强, 张西波 , 等. 18 079例胆石病患者临床分析[J]. 中国中西医结合外科杂志, 2006,12(4):324-327
doi: 10.3969/j.issn.1007-6948.2006.04.003
[6] Diehl AK . Epidemiology and natural history of gallstone disease[J]. Gastroenterol Clin North Am, 1991,20(1):1-19
doi: 10.1055/s-2008-1040469 pmid: 2022415
[7] Wang HH, Afdhal NH, Wang DQ . Estrogen receptor alpha, but not beta, plays a major role in 17beta-estradiol-induced murine cholesterol gallstones[J]. Gastroenterology, 2004,127(1):239-249
doi: 10.1053/j.gastro.2004.03.059
[8] Wang HH, Afdhal NH, Wang DQ . Overexpression of estrogen receptor alpha increases hepatic cholesterogenesis, leading to biliary hypersecretion in mice[J]. J Lipid Res, 2006,47(4):778-786
doi: 10.1194/jlr.M500454-JLR200
[9] Dhiman RK, Sarkar PK, Sharma A , et al. Alterations in gallbladder emptying and bile retention in the absence of changes in bile lithogenicity in postmenopausal women on hormone replacement therapy[J]. Dig Dis Sci, 2004,49(8):1335-1341
doi: 10.1023/B:DDAS.0000037831.77490.06
[10] Kline LW, Karpinski E . Progesterone inhibits gallbladder motility through multiple signaling pathways[J]. Steroids, 2005,70(9):673-679
doi: 10.1016/j.steroids.2005.03.011 pmid: 15916787
[11] Gilloteaux J, Karkare S, Ko W , et al. Female sex steroid induced epithelial changes in the gallbladder of the ovariectomized Syrian hamster[J]. Tissue Cell, 1992,24(6):869-878
doi: 10.1016/0040-8166(92)90022-Y
[12] Zhu PT, Zhang JZ, Cao ZP , et al. Summary of the treatment of 274 patients with cholelithiasis and chronic biliary tract infection[J]. Shanghai Zhong Yi Yao Za Zhi, 1986,20(9):15-17
朱培庭, 张静喆, 曹中平 , 等. 治疗慢性胆道感染、胆石病274例的总结[J]. 上海中医药杂志, 1986,20(9):15-17
[13] Gu HG, Zhang JZ, Zhang XL , et al. Effects of Shengqing Capsules on expression of nuclear factor-κB protein in liver cells of guinea pigs with pigment gallstone[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(5):518-521
顾宏刚, 张静喆, 章学林 , 等. 升清胶囊对胆色素结石豚鼠肝细胞核因子-κB蛋白表达的影响[J]. 中西医结合学报, 2006,4(5):518-521
[14] Fang BJ, Zhu PT, Zhang JZ , et al. Inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow on cholesterol gallstone formation in guinea pigs[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(1):56-59
方邦江, 朱培庭, 张静喆 , 等. 疏肝利胆中药防止胆固醇结石形成的实验研究[J]. 中西医结合学报, 2006,4(1):56-59
[15] Zhu PT, Zhang JZ, Zhang XL , et al. Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(3):207-210
朱培庭, 张静喆, 章学林 , 等. 升清胶囊对豚鼠胆石病相关基因的影响[J]. 中西医结合学报, 2005,3(3):207-210
[1] Jun Shi, Ye Lu, Xuan Zhang, Xiao-wei Wang, Min Ye, Jian-peng Jiao, Bei Pei, Pin-kang Wei. Relationship between expressions of estrogen and progesterone receptors and syndrome types of gastric carcinoma. Journal of Chinese Integrative Medicine, 2010, 8(7): 629-635.
[2] Jian-ye Yuan , De-gao Zhang, Xiao Yu, Da-zheng Wu. Effects of extracts and active components of Rhizoma Coptidis on contraction of circular smooth muscle isolated from guinea pig gastric antrum. Journal of Chinese Integrative Medicine, 2009, 7(9): 831-835.
[3] Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.
[4] Zheng-gao Xie, Xing-wei Wu, Chao-rong Zhuang, Fang Chen, Zheng Wang, Ya-kun Wang, Xin Hua. Protective effects of Ginkgo biloba extract on morphology and function of retinal ganglion cells after optic nerve transection in guinea pigs. Journal of Chinese Integrative Medicine, 2009, 7(10): 940-945.
[5] Xue-lin Zhang, Xiao-qiang Liang, Hong-gang Gu, En-wei Ma, Tian-bi Lin, Xun Sun, Jing-zhe Zhang. Effects of Shengqing Capsule on biochemical parameters in mice with cholesterol gallstone. Journal of Chinese Integrative Medicine, 2008, 6(10): 1045-1048.
[6] Ping Shen, Bang-jiang Fang, Pei-ting Zhu, Jing-zhe Zhang, Xin-jun Pei. Effect of traditional Chinese herbs for nourishing the liver on intracellular free calcium level in gallbladder cells of guinea pigs with gallstones. Journal of Chinese Integrative Medicine, 2007, 5(2): 179-182.
[7] Hong-gang Gu, Jing-zhe Zhang, Xue-lin Zhang, Jian-wen Liu, Ting Li, Pei-ting Zhu. Effects of Shengqing Capsules on expression of nuclear factor-κB protein in liver cells of guinea pigs with pigment gallstone. Journal of Chinese Integrative Medicine, 2006, 4(5): 518-521.
[8] Shu-fang Shi, Chao-qin Yu​. Progress in research on phytoestrogens and their effect targets. Journal of Chinese Integrative Medicine, 2005, 3(5): 408-410.
[9] Pei-ting Zhu, Jing-zhe Zhang, Xue-lin Zhang, Zuo-biao Yuan, Hong-ying Zhang, Ju Gao. Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs. Journal of Chinese Integrative Medicine, 2005, 3(3): 207-210.
[10] Gui-lin Ouyang, Qing-ming Yang, Lian-fu Deng, Fu-ping Xu, Ya-ping Zhu. Study on estrogen receptor of adult human osteoblasts. Journal of Chinese Integrative Medicine, 2003, 1(2): 132-134.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[5] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[6] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[7] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[8] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[9] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[10] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97