[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 56-60.doi: 10.3736/jcim20070111

• Original Experimental Research • Previous Articles     Next Articles

Inhibitory effects of 11 coumarin compounds against growth of human bladder carcinoma cell line E-J in vitro

Xiu-wei Yang, Bo Xu, Fu-xiang Ran, Rui-qing Wang, Jun Wu, Jing-rong Cui   

  1. The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100083, China
  • Online:2007-01-31 Published:2007-01-21

Objective: To screen antitumor active compounds, drug-like or leading compounds from Chinese traditional and herbal drugs.

Methods: Eleven coumarin compounds isolated from the Chinese traditional and herbal drugs were studied for their antitumor activities in vitro by determining the inhibition rates against growth of human bladder carcinoma cell line E-J.

Results: It showed that umbelliferone, scoparone, demethylfuropinarine, isopimpinellin, forbesoside, columbianadin, decursin and glycycoumarin inhibited the growth of human bladder carcinoma cell line E-J in vitro and their activities showed a concentration-effect relationship. The inhibitory effects of forbesoside, columbianadin, decursin and umbelliferone, with IC50 values of 7.50×10 -7, 2.30×10 -6, 6.00×10 -6 and 1.30×10 -6 mol/L, respectively, were stronger than those of the other tested compounds. However, xanthotoxin, esculin and sphondin did not inhibit the growth of human bladder carcinoma cell line E-J in this assay condition. 

Conclusion: These findings indicate that forbesoside, columbianadin, esculin, decursin and umbelliferone would be effective or regarded as potent drug-like or leading compounds against human bladder carcinoma.

Key words: Chinese herbal drugs, coumarins, bladder neoplasms

CLC Number: 

  • R737.11

Table 1

Name, source and contained part in plant of 11 coumarins compounds"

No. Name Source Part
1 Umbelliferone 狭叶当归Angelica anomala Root
2 Esculin 日本白蜡树Fraxinus japonica Stem bark
3 Scoparone 茵陈蒿Artemisia capillaris Whole plant
4 Demethylfuropinarine 狭叶羌活Notopterygium incisum Underground part
5 Isopimpinellin 东当归Angelica acutiloba Fruit
6 Xanthotoxin 东当归Angelica acutiloba Fruit
7 Forbesoside 狭叶羌活Notopterygium incisum Underground part
8 Columbianadin 大活Angelica dahurica Root
9 Sphondin 软毛独活Heracleum lanatum Root
10 Decursin 朝鲜当归Angelica gigas Root
11 Glycycoumarin 乌拉尔甘草Glycyrrhiza uralensis Root

Figure 1

Structures of 11 tested coumarin compounds"

Figure 2

Concentration-effect curves of tested compounds against growth of human bladder carcinoma cell line E-J ○:1; ◆:3; ■:4; ▲:5; :7; :8; □:10; ●:11."

Table 2

IC50 values of coumarin compounds against growth of human bladder carcinoma cell line E-J"

No. Compound name IC50 (mol/L)
1 Umbelliferone 1.30×10-6
3 Scoparone 1.80×10-5
4 Demethylfuropinarine 1.60×10-5
5 Isopimpinellin 4.70×10-5
7 Forbesoside 7.50×10-7
8 Columbianadin 2.30×10-6
10 Decursin 6.00×10-6
11 Glycycoumarin 1.90×10-5
[1] 杨秀伟, 严仲铠, 顾哲明 , 等. 羌活化学成分的研究. 中草药, 1993,24(10):507-511
Yang XW, Yan ZK, Gu ZM , et al. Studies on the chemical constituents of incised Notopterygium(Notopterygium incisum). Zhong Cao Yao, 1993,24(10):507-511
[2] 杨秀伟, 严仲铠, 顾哲明 , 等. 宽叶羌活化学成分的研究. 中国药学杂志, 1994,29(3):141-143
Yang XW, Yan ZK, Gu ZM , et al. Studies on the chemical constituents of forbes Notopterygium(Notopterygium forbesii). Zhongguo Yao Xue Za Zhi, 1994,29(3):141-143
[3] Lou HX, Sun LR, Yu WT , et al. Absolute configuration determination of angular dihydro-coumarins from Peucedanum praeruptorum. J Asian Nat Prod Res, 2004,6(3):177-184
doi: 10.1080/10286020310001653255
[4] 杨秀伟, 王继彦, 严仲铠 . 四种长白山产当归属药用植物化学成分的研究. 中药材, 1994,17(4):30-32
Yang XW, Wang JY, Yan ZK . Studies on the coumarin constituents of four species in genus Angelica from Changbai moutain. Zhong Yao Cai, 1994,17(4):30-32
[5] Yang XW, Hattori M, Namba T . Two new coumarins from the roots of Aegle marmelos. J Chin Pharm Sci, 1996,5(2):68-73
[6] Yang XW, Hattori M, Namba T . Studies on the anti-lipid peroxidative actions of the methanolic extract of the root of Aegle marmelos and its constituents in vivo and in vitro. J Chin Pharm Sci, 1996,5(3):132-140
[7] McCulloch P, George WD . Warfarin inhibits metastasis of Mtln3 rat mammary carcinoma without affecting primary tumour growth. Br J Cancer, 1989,59(2):179-183
[8] Fuskova A, Proksa B, Fuska J . In vitro effect of duclauxin and derivatives of coumarin on nucleic acid and protein synthesis in Ehrlich's Ascites Carcinoma cells(EAC). Pharmazie, 1977,32(5):291-293
[9] 胡昌奇, 李国雄 . 竹叶防风中的香豆素类及其抑制DNA拓扑异构酶II的作用. 天然产物研究与开发, 1992,4(1):6-10
Hu CQ, Lee KS . Coumarins from the roots of Seseli mairei and inhibition of DNA topoisomerase II activity. Tian Ran Chan Wu Yan Jiu Yu Kai Fa, 1992,4(1):6-10
[10] 杨秀伟, 徐波, 吴军 , 等. 40种香豆素类化合物对人鼻咽癌细胞株KB和人白血病细胞株HL-60细胞生长抑制活性的筛选. 中国现代中药, 2006,8(10):8-13
Yang XW, Xu B, Wu J , et al. Inhibitory effects of 40 coumarins compounds against growth of human nasopharyngeal carcinoma cell line KB and human leukemia cell line HL-60 in vitro. Zhongguo Xian Dai Zhong Yao, 2006,8(10):8-13
[11] Monks A, Scudiero D, Skehan P , et al. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst, 1991,83(11):757-756
[12] Gu ZM, Zhang DX, Yang XW , et al. Isolation of two new coumarin glycosides from Notopterygium forbesii and evaluation of a Chinese crude drug, Qiang-Huo, the underground parts of N. incisum and N. forbesii, by high-performance liquid chromatography. Chem Pharm Bull, 1990,38(9):2498-2502
[13] Sato Y, Akao T, He JX , et al. Glycycoumarin from Glycyrrhizae Radix acts as a potent antispasmodic through inhibition of phosphodiesterase 3. J Ethnopharmacol, 2006,105(3):409-414
[14] Jiang C, Lee HJ, Li GX , et al. Potent antiandrogen and androgen receptor activities of an Angelica gigas-containing herbal formulation: identification of decursin as a novel and active compound with implications for prevention and treatment of prostate cancer. Cancer Res, 2006,66(1):453-463
[15] Ahn KS, Sim WS, Kim IH . Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med, 1996,62(1):7-9
doi: 10.1055/s-2006-957785 pmid: 8720379
[16] Yim D, Singh RP, Agarwal C , et al. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res, 2005,65(3):1035-1044
[17] Kim JH, Jeong JH, Jeon ST , et al. Decursin inhibits induction of inflammatory mediators by blocking nuclear factor-kappaB activation in macrophages. Mol Pharmacol, 2006,69(6):1783-1790
[1] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[2] Lu Yu, Shui-miao Lin, Ru-qian Zhou, Wei-jun Tang, Pin-xian Huang, Ying Dong, Jian Wang, Zhi-hua Yu, Jiu-lin Chen, Li Wei, San-li Xing, Hui-juan Cao, Hong-bin Zhao. Chinese herbal medicine for patients with mild to moderate Alzheimer disease based on syndrome differentiation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(7): 766-776.
[3] Ling Xu, Qiao-ling Zhou, Jie Han, Dan-ying Zhang, Hui-juan Dong, Zai-long Cai, Chao-qin Yu, Dong-xia Zhai . Effects of Chinese herbal medicine Neiyi Recipe-medicated serum on angiopoiesis of endometriosis in the chick chorioallantoic membrane model. Journal of Chinese Integrative Medicine, 2012, 10(7): 800-806.
[4] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[5] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[6] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[7] Shui-qiu Deng, Xue-nong Ouyang, Zong-yang Yu, Xi-hu Dai, Xi Chen, Fang-zheng Fang, Wen-wu Wang, Zhi-zhen Liu. Influence of Chinese herbal medicine Feitai Capsule on completion or delay of chemotherapy in patients with stage ⅢB/Ⅳ non-small-cell lung cancer: a Randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(6): 635-640.
[8] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[9] Hui Luo, Jian-ping Liu, Qing Li. Methodological issues and suggestions for improvement in randomized controlled trials of Chinese herbal medicine for recurrent miscarriage. Journal of Chinese Integrative Medicine, 2012, 10(6): 604-614.
[10] Daniel A. Weber, Janelle M. Wheat , Geoffrey M. Currie. Cancer stem cells and the impact of Chinese herbs, isolates and other complementary medical botanicals: A review. Journal of Chinese Integrative Medicine, 2012, 10(5): 493-503.
[11] Jing Guo, Ju-yong Wang, Zhan Zheng, Qing Wang, Chang-sheng Dong . Effects of Chinese herbal medicine Feiyanning Decoction on the ratio of CD4+CD25+ regulatory T cells and expression of transcription factor Foxp3 in mice bearing Lewis lung carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(5): 584-590.
[12] Qing-lan Wang, Yan-yan Tao , Li Shen , Hong-yan Cui , Cheng-hai Liu. Chinese herbal medicine Fuzheng Huayu recipe inhibits liver fibrosis by mediating the transforming growth factor-β1/Smads signaling pathway. Journal of Chinese Integrative Medicine, 2012, 10(5): 561-568.
[13] Zhan gheng, Ju-yong Wang, Qing Wang, Ling Xu, Zhen-ye Xu. Effects of Chinese herbal medicine Feiyanning decoction on expressions of nucleosome conformation-regulating factors H3-K56, Rtt109, Asf1 and E2F1 in Lewis-bearing mice. Journal of Chinese Integrative Medicine, 2012, 10(4): 448-453.
[14] Ying-chun Miao , Jin-zhou Tian , Jing Shi , Min Mao. Effects of Chinese medicine for tonifying the kidney and resolving phlegm and blood stasis in treating patients with amnestic mild cognitive impairment: A randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(4): 390-397.
[15] Na Mu-hai, Yao Cheng-zeng, Yu Xiao-tian, Jiang Mei-xian. Effects of added therapy with Chinese herbal medicine Kanli Granule on skeletal muscle dysfunction in patients with chronic heart failure. Journal of Chinese Integrative Medicine, 2012, 10(3): 324-329.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]