11种香豆素类化合物对人膀胱癌细胞系E-J细胞株生长抑制活性的筛选

doi:10.3736/jcim20070111

Abstract

Objective: To screen antitumor active compounds, drug-like or leading compounds from Chinese traditional and herbal drugs.

Methods: Eleven coumarin compounds isolated from the Chinese traditional and herbal drugs were studied for their antitumor activities in vitro by determining the inhibition rates against growth of human bladder carcinoma cell line E-J.

Results: It showed that umbelliferone, scoparone, demethylfuropinarine, isopimpinellin, forbesoside, columbianadin, decursin and glycycoumarin inhibited the growth of human bladder carcinoma cell line E-J in vitro and their activities showed a concentration-effect relationship. The inhibitory effects of forbesoside, columbianadin, decursin and umbelliferone, with IC50 values of 7.50×10 ^-7, 2.30×10 ^-6, 6.00×10 ^-6 and 1.30×10 ^-6 mol/L, respectively, were stronger than those of the other tested compounds. However, xanthotoxin, esculin and sphondin did not inhibit the growth of human bladder carcinoma cell line E-J in this assay condition.

Conclusion: These findings indicate that forbesoside, columbianadin, esculin, decursin and umbelliferone would be effective or regarded as potent drug-like or leading compounds against human bladder carcinoma.

Key words: Chinese herbal drugs, coumarins, bladder neoplasms

CLC Number:

R737.11

Xiu-wei Yang, Bo Xu, Fu-xiang Ran, Rui-qing Wang, Jun Wu, Jing-rong Cui. Inhibitory effects of 11 coumarin compounds against growth of human bladder carcinoma cell line E-J in vitro. Journal of Chinese Integrative Medicine, 2007, 5(1): 56-60.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070111

http://www.jcimjournal.com/EN/Y2007/V5/I1/56

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References 17

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No.	Name	Source	Part
1	Umbelliferone	狭叶当归Angelica anomala	Root
2	Esculin	日本白蜡树Fraxinus japonica	Stem bark
3	Scoparone	茵陈蒿Artemisia capillaris	Whole plant
4	Demethylfuropinarine	狭叶羌活Notopterygium incisum	Underground part
5	Isopimpinellin	东当归Angelica acutiloba	Fruit
6	Xanthotoxin	东当归Angelica acutiloba	Fruit
7	Forbesoside	狭叶羌活Notopterygium incisum	Underground part
8	Columbianadin	大活Angelica dahurica	Root
9	Sphondin	软毛独活Heracleum lanatum	Root
10	Decursin	朝鲜当归Angelica gigas	Root
11	Glycycoumarin	乌拉尔甘草Glycyrrhiza uralensis	Root

No.	Compound name	IC₅₀ (mol/L)
1	Umbelliferone	1.30×10-6
3	Scoparone	1.80×10-5
4	Demethylfuropinarine	1.60×10-5
5	Isopimpinellin	4.70×10-5
7	Forbesoside	7.50×10-7
8	Columbianadin	2.30×10-6
10	Decursin	6.00×10-6
11	Glycycoumarin	1.90×10-5

Inhibitory effects of 11 coumarin compounds against growth of human bladder carcinoma cell line E-J in vitro

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