Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 56-60.doi: 10.3736/jcim20070111

• Original Experimental Research • Previous Articles     Next Articles

Inhibitory effects of 11 coumarin compounds against growth of human bladder carcinoma cell line E-J in vitro

Xiu-wei Yang, Bo Xu, Fu-xiang Ran, Rui-qing Wang, Jun Wu, Jing-rong Cui   

  1. The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100083, China
  • Online:2007-01-31 Published:2007-01-21

Objective: To screen antitumor active compounds, drug-like or leading compounds from Chinese traditional and herbal drugs.

Methods: Eleven coumarin compounds isolated from the Chinese traditional and herbal drugs were studied for their antitumor activities in vitro by determining the inhibition rates against growth of human bladder carcinoma cell line E-J.

Results: It showed that umbelliferone, scoparone, demethylfuropinarine, isopimpinellin, forbesoside, columbianadin, decursin and glycycoumarin inhibited the growth of human bladder carcinoma cell line E-J in vitro and their activities showed a concentration-effect relationship. The inhibitory effects of forbesoside, columbianadin, decursin and umbelliferone, with IC50 values of 7.50×10 -7, 2.30×10 -6, 6.00×10 -6 and 1.30×10 -6 mol/L, respectively, were stronger than those of the other tested compounds. However, xanthotoxin, esculin and sphondin did not inhibit the growth of human bladder carcinoma cell line E-J in this assay condition. 

Conclusion: These findings indicate that forbesoside, columbianadin, esculin, decursin and umbelliferone would be effective or regarded as potent drug-like or leading compounds against human bladder carcinoma.

Key words: Chinese herbal drugs, coumarins, bladder neoplasms

CLC Number: 

  • R737.11

Table 1

Name, source and contained part in plant of 11 coumarins compounds"

No. Name Source Part
1 Umbelliferone 狭叶当归Angelica anomala Root
2 Esculin 日本白蜡树Fraxinus japonica Stem bark
3 Scoparone 茵陈蒿Artemisia capillaris Whole plant
4 Demethylfuropinarine 狭叶羌活Notopterygium incisum Underground part
5 Isopimpinellin 东当归Angelica acutiloba Fruit
6 Xanthotoxin 东当归Angelica acutiloba Fruit
7 Forbesoside 狭叶羌活Notopterygium incisum Underground part
8 Columbianadin 大活Angelica dahurica Root
9 Sphondin 软毛独活Heracleum lanatum Root
10 Decursin 朝鲜当归Angelica gigas Root
11 Glycycoumarin 乌拉尔甘草Glycyrrhiza uralensis Root

Figure 1

Structures of 11 tested coumarin compounds"

Figure 2

Concentration-effect curves of tested compounds against growth of human bladder carcinoma cell line E-J ○:1; ◆:3; ■:4; ▲:5; :7; :8; □:10; ●:11."

Table 2

IC50 values of coumarin compounds against growth of human bladder carcinoma cell line E-J"

No. Compound name IC50 (mol/L)
1 Umbelliferone 1.30×10-6
3 Scoparone 1.80×10-5
4 Demethylfuropinarine 1.60×10-5
5 Isopimpinellin 4.70×10-5
7 Forbesoside 7.50×10-7
8 Columbianadin 2.30×10-6
10 Decursin 6.00×10-6
11 Glycycoumarin 1.90×10-5
[1] 杨秀伟, 严仲铠, 顾哲明 , 等. 羌活化学成分的研究. 中草药, 1993,24(10):507-511
Yang XW, Yan ZK, Gu ZM , et al. Studies on the chemical constituents of incised Notopterygium(Notopterygium incisum). Zhong Cao Yao, 1993,24(10):507-511
[2] 杨秀伟, 严仲铠, 顾哲明 , 等. 宽叶羌活化学成分的研究. 中国药学杂志, 1994,29(3):141-143
Yang XW, Yan ZK, Gu ZM , et al. Studies on the chemical constituents of forbes Notopterygium(Notopterygium forbesii). Zhongguo Yao Xue Za Zhi, 1994,29(3):141-143
[3] Lou HX, Sun LR, Yu WT , et al. Absolute configuration determination of angular dihydro-coumarins from Peucedanum praeruptorum. J Asian Nat Prod Res, 2004,6(3):177-184
doi: 10.1080/10286020310001653255
[4] 杨秀伟, 王继彦, 严仲铠 . 四种长白山产当归属药用植物化学成分的研究. 中药材, 1994,17(4):30-32
Yang XW, Wang JY, Yan ZK . Studies on the coumarin constituents of four species in genus Angelica from Changbai moutain. Zhong Yao Cai, 1994,17(4):30-32
[5] Yang XW, Hattori M, Namba T . Two new coumarins from the roots of Aegle marmelos. J Chin Pharm Sci, 1996,5(2):68-73
[6] Yang XW, Hattori M, Namba T . Studies on the anti-lipid peroxidative actions of the methanolic extract of the root of Aegle marmelos and its constituents in vivo and in vitro. J Chin Pharm Sci, 1996,5(3):132-140
[7] McCulloch P, George WD . Warfarin inhibits metastasis of Mtln3 rat mammary carcinoma without affecting primary tumour growth. Br J Cancer, 1989,59(2):179-183
[8] Fuskova A, Proksa B, Fuska J . In vitro effect of duclauxin and derivatives of coumarin on nucleic acid and protein synthesis in Ehrlich's Ascites Carcinoma cells(EAC). Pharmazie, 1977,32(5):291-293
[9] 胡昌奇, 李国雄 . 竹叶防风中的香豆素类及其抑制DNA拓扑异构酶II的作用. 天然产物研究与开发, 1992,4(1):6-10
Hu CQ, Lee KS . Coumarins from the roots of Seseli mairei and inhibition of DNA topoisomerase II activity. Tian Ran Chan Wu Yan Jiu Yu Kai Fa, 1992,4(1):6-10
[10] 杨秀伟, 徐波, 吴军 , 等. 40种香豆素类化合物对人鼻咽癌细胞株KB和人白血病细胞株HL-60细胞生长抑制活性的筛选. 中国现代中药, 2006,8(10):8-13
Yang XW, Xu B, Wu J , et al. Inhibitory effects of 40 coumarins compounds against growth of human nasopharyngeal carcinoma cell line KB and human leukemia cell line HL-60 in vitro. Zhongguo Xian Dai Zhong Yao, 2006,8(10):8-13
[11] Monks A, Scudiero D, Skehan P , et al. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst, 1991,83(11):757-756
[12] Gu ZM, Zhang DX, Yang XW , et al. Isolation of two new coumarin glycosides from Notopterygium forbesii and evaluation of a Chinese crude drug, Qiang-Huo, the underground parts of N. incisum and N. forbesii, by high-performance liquid chromatography. Chem Pharm Bull, 1990,38(9):2498-2502
[13] Sato Y, Akao T, He JX , et al. Glycycoumarin from Glycyrrhizae Radix acts as a potent antispasmodic through inhibition of phosphodiesterase 3. J Ethnopharmacol, 2006,105(3):409-414
[14] Jiang C, Lee HJ, Li GX , et al. Potent antiandrogen and androgen receptor activities of an Angelica gigas-containing herbal formulation: identification of decursin as a novel and active compound with implications for prevention and treatment of prostate cancer. Cancer Res, 2006,66(1):453-463
[15] Ahn KS, Sim WS, Kim IH . Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med, 1996,62(1):7-9
doi: 10.1055/s-2006-957785 pmid: 8720379
[16] Yim D, Singh RP, Agarwal C , et al. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res, 2005,65(3):1035-1044
[17] Kim JH, Jeong JH, Jeon ST , et al. Decursin inhibits induction of inflammatory mediators by blocking nuclear factor-kappaB activation in macrophages. Mol Pharmacol, 2006,69(6):1783-1790
[1] Pragya Sharma, Sonali Batra, Ashwani Kumar, Anupam Sharma. In vivo antianxiety and antidepressant activity of Murraya paniculata leaf extracts. Journal of Integrative Medicine, 2017, 15(4): 320-325.
[2] Ming-hui Yang, Min Li, Yong-qi Dou, Yi Liu, Xiao-dong Luo, Jian-zong Chen, Heng-jun Shi. Effects of Bushen Huoxue Granule on motor function in patients with Parkinson’s disease: A multicenter, randomized, double-blind and placebo-controlled trial. Journal of Chinese Integrative Medicine, 2010, 8(3): 231-237.
[3] Ya-bing Zhou, Jian-er Yu, Jie Wu, Li Bai, Li-li Huo, Xin-guang Zhang, Li-qing Li . Effects of Chinese herbal medicine Bushen Gubiao Recipe on toll-like receptor 4 and CD4 +CD25 +foxp3 +regulatory T cells in mice with recurrent respiratory tract infections. Journal of Chinese Integrative Medicine, 2010, 8(11): 1053-1059.
[4] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052.
[5] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047.
[6] Wen-jun Wang, Da-jin Li, Jun Li, Wen-jiang Zhou. An in vitro study on neuroprotective effects of serum containing Gengnianchun decoction and its main monomers against amyloid beta protein-induced cellular toxicity. Journal of Chinese Integrative Medicine, 2010, 8(1): 67-73.
[7] Li Yan, Xian-chuan Chen, Jian Guo, Li-li Qi, Yi-ming Qian, Xiong-gen Zhou. Prevention of hospital-acquired pneumonia with Yupingfeng Powder in patients with acute cerebral vascular diseases: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2010, 8(1): 25-29.
[8] Xin-yue Zhi . Traditional Chinese medicine diagnosis and treatment of type 2 diabetes in Tianjin urban population. Journal of Chinese Integrative Medicine, 2009, 7(9): 823-826.
[9] Deng-hao Liu, Zheng Wang, Zhang-yan Qin. Clinical effects of Shugan Jianpi Recipe on postadolescent acne in women. Journal of Chinese Integrative Medicine, 2009, 7(7): 671-673.
[10] Xin-jun Liu, Shi-bing Su. Study of combination methods for formula composition of Chinese herbal medicines and their components. Journal of Chinese Integrative Medicine, 2009, 7(7): 601-606.
[11] Xian-jun Tong, Xiao-yu Xiang, Zun-hua Cheng, Li Chen, Xian-chuan Chen, Jing-xian Chen. Color Doppler ultrasound evaluation of a Chinese herbal formula in treating lower limb arteriosclerosis: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2009, 7(6): 518-521.
[12] Ai-ping Lu, Xue-wen Liu, Xiao-rong Ding. Methodology of pharmacodynamic evaluation on Chinese herbal medicine based on syndrome differentiation. Journal of Chinese Integrative Medicine, 2009, 7(6): 501-504.
[13] Jia Guo , Rui-ming Zhang, Zong-wen Huang, Lei Wang, Song-shan Liu, Jun-cheng Diao, Si-yuan Hu, Bing-hui Lin. Yunchang Capsule in treatment of functional constipation: A randomized, double-blinded controlled, multicenter trial. Journal of Chinese Integrative Medicine, 2009, 7(12): 1123-1129.
[14] Wen Cao, Ai-guang Zhao . Prescription rules of Chinese herbal medicines in treatment of gastric cancer. Journal of Chinese Integrative Medicine, 2009, 7(1): 1-8.
[15] Chung-wah Cheng, Zhao-xiang Bian, You-ping Li, David Moher, Tai-xiang Wu, Simon Dagenais, Jing Li, Ting-qian Li. Transparently reporting adverse effects of traditional Chinese medicine interventions in randomized controlled trials. Journal of Chinese Integrative Medicine, 2008, 6(9): 881-886.
Full text



[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022