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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 322-327.doi: 10.3736/jcim20070318

• Original Experimental Research • Previous Articles     Next Articles

Ganoderma spores may regulate the levels of mitochondria-related molecular substances in hippocampus of young rats birthed by rats with gestational hypertension

Jin-jun Cheng, Yuan-shan Zeng, Yi Xiong, Wei Zhang, Sui-jun Chen, Zhi-qiang Zhong   

  1. Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, China
  • Online:2007-05-31 Published:2007-05-15

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Objective: To investigate the effects of Ganoderma spores on mitochondria-related molecular substances in hippocampus of young rats birthed by rats with gestational hypertension.Methods: Nitric oxide synthase (NOS) inhibitor Nw-nitro-L-arginine methylester (L-NAME) was intraperitoneally injected into pregnant rats to induce gestational hypertension, and Ganoderma spores were administered orally. The effects of Ganoderma spores on levels of mitochondria-related molecular substances in hippocampus of young rats birthed by the rats with gestational hypertension were evaluated with immunoradiometric assay of cAMP, RT-PCR analysis of related genes, and detection of enzyme activity.Result: In hippocampus of the new-born rats birthed by rats with gestational hypertension, the cAMP level, mitochondrial DNA (mtDNA) level and adenosine triphosphatase (ATPase) activity were decreased, and the expression level of peroxisome proliferator activated receptor gamma coactivator 1 alpha (pgc1α) was unchanged compared to the normal control group. The cAMP level, mtDNA level, ATPase activity and pgc1α expression level in hippocampus of 30-day post-natal rats were lower than those of the rats in normal control group. After oral administration of Ganoderma spores, the cAMP and mtDNA levels in hippocampus of the new-born rats and 30-day post-natal rats recovered almost to the levels of normal control rats, and the ATPase activity and pgc1α expression level were also increased significantly.Conclusion: Ganoderma spores may regulate the levels of mitochondria-related molecular substances in hippocampus of young rats birthed by rats with gestational hypertension.

Key words: rat, Ganoderma spores, gestational hypertension, hippocampus, mitochondria, mitochondrial DNA

CLC Number: 

  • R651.21

Table 1

cAMP level in hippocampus of P0 and P30 young rats （ヌ±S）"

Group of pregnant rats n cAMP level of young rats
P0 P30
Normal control 3 2.57±0.27** 1.73±0.40*
L-NAME+distilled water 3 1.07±0.44 1.17±0.27
L-NAME+arginine 3 1.30±0.39 1.75±0.13*
L-NAME+GS 3 2.93±0.16** 1.80±0.42*
GS+L-NAME+GS 3 2.98±0.55** 1.83±0.60*

Figure 1

Expression levels of pgclα and tfam determined by RT-PCRn: Normal control group, P0; m: L-NAME+distilled water group, P0; a: L-NAME+arginine group, P0; g: L-NAME+GS group, P0; p: GS +L-NAME+GS group, P0; N: Normal control group, P30; M: L-NAME+distilled water group, P30; A: L-NAME+arginine group, P30; G: L-NAME+GS group, P30; P: GS+L-NAME+GS group, P30; L: 100 bp DNA marker. n=3. P0: new-born rats; P30: 30-day post-natal rats."

Table 2

Expression levels of mitochondrial biogenesis related genes in hippocampus of P0（ヌ±S）"

Group of pregnant rats n tfam expression of young rats pgclα expression of young rats
P0 P30 P0 P30
Normal control 3 1.20±0.12△△ 0.92±0.12△△ 1.87±0.19 2.77±0.24△△
L-NAME+distilled water 0.58±0.21 0.46±0.02 1.14±0.05 0.97±0.10
L-NAME+arginine 0.99±0.01△ 0.89±0.13 0.82±0.18* 1.19±0.44**
L-NAME+GS 1.18±0.21△△ 0.73±0.11△△ 2.86±0.32*△△ 1.93±0.19△△
GS+L-NAME+GS 0.86±0.04 0.87±0.02△△ 1.51±0.21 1.88±0.58△△

Figure 2

COX3 and COX4 gene amplification through PCRL: 100 bp DNA marker; n: Normal control group, P0; m: L-NAME+distilled water group, P0; a: L-NAME+arginine group, P0; g: L-NAME+GS group, P0; p: GS+L-NAME+GS group, P0; N: Normal control group, P30; M: L-NAME+distilled water group, P30; A: L-NAME+arginine group, P30; G: L-NAME+GS group, P30; P: GS+L-NAME+GS group, P30. n=3. P0: new-born rats; P30: 30-day post-natal rats."

Table 4

Activities of metabolism related enzymes in hippocampus of P0 young rats （ヌ±S）"

Group of pregnant rats n Enzyme activity of P0 young rats
LDH (U/g prot) PK(U/g prot) GS (U/mg prot) Lactate (mmol/g prot) ATPase [μmol pi/(mg prot·h)]
Normal control 3 7 916.6±233.2△ 153.2±8.3△△ 170.3±5.9△△ 0.56±0.08 1.57±0.09△△
L-NAME+distilled water 3 9 141.9±611.4 229.6±9.6 106.8±9.3 0.73±0.06 0.54±0.22
L-NAME+arginine 3 8 708.9±380.1 231.2±20.3** 107.7±14.9** 0.73±0.08 0.70±0.12**
L-NAME+GS 3 6 851.7±658.6△△ 178.2±21.8△ 141.7±8.3*△△ 0.66±0.05 1.13±0.08*△△
GS+L-NAME+GS 3 6 913.7±187.1△△ 175.5±19.8△ 148.8±7.1△△ 0.56±0.05△ 1.06±0.19*△

Table 5

Activities of metabolism related enzymes in hippocampus of P30 young rats （ヌ±S）"

Group of pregnant rats n Enzyme activity of P30 young rats
LDH (U/g prot) PK (U/g prot) GS (U/mg prot) Lactate (mmol/g prot) ATPase [(μmol pi/(mg prot·h)]
Normal control 6 347.9±202.6 73.3±17.6 87.5±5.6△ 0.48±0.05 3.25±0.07△△
L-NAME+distilled water 5 317.7±303.1 67.5±15.5 61.5±8.6 0.62±0.04 2.51±0.37
L-NAME+arginine 5 573.1±162.9 68.5±6.8 71.8±3.4 0.73±0.05** 2.70±0.19*
L-NAME+GS 6 692.4±439.7△ 56.7±7.8 95.9±4.7△△ 0.39±0.04△ 3.17±0.11△△
GS+L-NAME+GS 6 805.6±224.4△ 49.5±6.2 88.5±6.5△△ 0.46±0.03△ 3.24±0.10△△
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