[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 437-441.doi: 10.3736/jcim20070415

• Original Experimental Research • Previous Articles     Next Articles

Effects of warming spleen and stomach for dispersing phlegm recipe on expression of interleukin-8 in S180 tumor tissue in mice

Hai-zhen Dang, Pin-kang Wei, Xiao-feng Zhang, Zhan-wen Liu   

  1. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Online:2007-07-31 Published:2007-05-15

RichHTML

0

PDF (PC)

96

Like

Objective: To observe the effects of warming spleen and stomach for dispersing phlegm recipe (Wenzhong Xiaotan Recipe, WZXTR), a compound traditional Chinese herbal medicine, on expression of interleukin-8 (IL-8) in S180 tumor tissue in mice, and to explore the effectiveness and its mechanism.Methods: Fifty mice were randomly divided into five groups: untreated group, cold water-treated group, cold water and WZXTR-treated group, cold water and dispersing phlegm and eliminating stagnation recipe (Xiaotan Sanjie Recipe, XTSJR, another compound traditional Chinese herbal medicine) treated group, cold water and tegafur-treated group. Mice in the latter 4 groups were fed 0-4 ℃ cold distilled water 10 ml/(kg·d) one week before tumor inoculation for 3 weeks. Mice in the 5 groups were inoculated neoplastic cells which were diluted 2×107/ml with normal saline in the right armpit at the second week. Forty-eight hours later, the mice were given drugs intragastrically (WZXTR, XTSJR and tegafur, respectively). The mice were continually intervened by cold water in the morning and given drugs in the afternoon. Mice in the cold water-treated group were given normal saline, and nothing was given in untreated group. Morphology appearance and changes of rectal temperature were observed, and the tumor weight and volume were measured and inhibitory rate was calculated 22 days after tumor inoculation. IL-8 was detected by avidin-biotin system-enzyme-labeled immunosorbent assay (ABC-ELISA) and real-time reverse polymerase chain reaction (real-time PCR).Result: The content of IL-8 in tumor tissue of the untreated group was significantly higher than that of the other groups (P＜0.01), and that of the cold water-treated group was higher than that of the cold water and WZXTR-treated group (P＜0.01) and the cold water and XTSJR-treated group (P＜0.05), respectively. The gray scale value of cDNA amplification strap was descent more evidently in both the cold water and WZXTR-treated group and the cold water and XTSJR-treated group as compared with that of the cold water-treated group and the untreated group (P＜0.05).Conclusion: WZXTR can restrain tumor growth, and the mechanism may be that it can degrade the expression of IL-8 in S180 tumor.

Key words: tumor, interleukin-8, mice, traditional Chinese medicine

CLC Number: 

  • 1977(2007)04-0437

Table 1

Tumor weight, volume and inhibitory rate of transplanted tumor"

Groupn Tumor weight (x±s, g)Tumor volume (x±s, cm3)Inhibitory rate (%)
Untreated102.41±0.452.67±0.17
Cold water-treated102.63±0.283.38±0.31
Cold water and XTSJR-treated101.02±0.02**△△1.08±0.02**△△61.18
Cold water and WZXTR-treated100.97±0.03**△△1.12±0.04**△△63.23
Cold water and tegafur-treated101.19±0.03**△△1.12±0.02**△△54.71

Table 2

Changes of IL-8 in tumor tissue of transplanted tumor (ヌ±S, pg/mg)"

Groupn IL-8
Untreated1029.94±3.52
Cold water-treated1019.24±8.12**▲▲
Cold water and XTSJR-treated1011.37±6.61**△
Cold water and WZXTR-treated109.35±5.51**
Cold water and tegafur-treated1018.63±3.81**▲▲

Figure 1

Expression of IL-8 mRNA in S180 tumor tissue1: Cold water and tegafur-treated group; 2: Untreated group; 3: Cold water and WZXTR-treated group; 4: Cold water and XTSJR-treated group; 5: Cold water-treated group."

Table 3

Positive expression of gene fragment of IL-8（ヌ±S）"

Groupn Positive expression of
gene fragment of IL-8
Untreated100.713±0.045
Cold water-treated100.622±0.060
Cold water and XTSJR-treated100.354±0.042*△
Cold water and WZXTR-treated100.242±0.024*△
Cold water and tegafur-treated100.532±0.077*△
[1] 陈艳芬, 陈蔚文, 李茹柳.寒、热型胃黏膜损伤模型的对比和应用研究[J].广东药学院学报,2005,21(3):290-291, 294
Chen YF, Chen WW, Li RL.Comparison study of cold or hot gastric mucosa lesion animal models[J].Guangdong Yao Xue Yuan Xue Bao,2005,21(3):290-291, 294
[2] 党海珍, 魏品康, 张霄峰.冰盐水对苯并(a)芘诱发小鼠前胃组织癌变过程的影响[J].第二军医大学学报,2006,27(11):1266-1267
doi: 10.3321/j.issn:0258-879X.2006.11.030
Dang HZ, Wei PK, Zhang XF.Influence of ice brine on the process of B(a) P inducing anterior stomach neoplasms in mice.[J].Di Er Jun Yi Da Xue Xue Bao,2006,27(11):1266-1267
doi: 10.3321/j.issn:0258-879X.2006.11.030
[3] Kitadai Y, Haruma K, Mukaida M, et al.Regulation of disease progression genes in human gastric carcimoma cells by interleukin-8[J].Clin Cancer Res,2000,7(6):2735-2740
[4] 郭晓临, 李莉, 袁媛.白介素-8在胃癌的表达及其与cag AHp感染的相关性研究[J].中华肿瘤杂志,2003,25(2):180-181
Guo XL, Li L, Yuan Y.Expression of IL-8 in gastric cancer and its correlation with infection of cag AHp[J].Zhonghua Zhong Liu Za Zhi,2003,25(2):180-181
[5] Luppi F, Longo AM, de Boer WI, et al.Interleukin-8 stimulates cell proliferation in non-small cell lung cancer through epidermal growth factor receptor transactivation[J].Lung Cancer,2007,56(1):25-33
[6] Maulik G, Shrikhande A, Kijima T, et al.Role of the hepatocyte growth factor receptor, c-Met, in oncogenesis and potential for therapeutic inhibition[J].Cytokine Growth Factor Rev,2002,13((1):41-59
[7] 陈亚琳, 魏品康, 许玲, 等.消痰散结方对胃癌组织中P21ras及P185蛋白表达的影响[J].山东中医药大学学报,2003,27(5):374-376
Chen YL, Wei PK, Xu L, et al.Effect of Xiaotan Sanjie recipe on expression of P21ras and P185 in gastric carcinoma[J].Shandong Zhong Yi Yao Da Xue Xue Bao,2003,27(5):374-376
[1] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
[2] Yong Zhang, Xiao-wen Sun, Jian-hua Xu, Hai Lu, Zhong-ze Fan, Jue Sun, Xiao-xiao Zhang. Effects of medicated serum prepared with Chinese herbal medicine Changweiqing on pharmacokinetics of oxaliplatin in colon cancer cells. Journal of Chinese Integrative Medicine, 2012, 10(8): 901-910.
[3] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[4] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[5] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[6] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[7] Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
[8] Shui-qiu Deng, Xue-nong Ouyang, Zong-yang Yu, Xi-hu Dai, Xi Chen, Fang-zheng Fang, Wen-wu Wang, Zhi-zhen Liu. Influence of Chinese herbal medicine Feitai Capsule on completion or delay of chemotherapy in patients with stage ⅢB/Ⅳ non-small-cell lung cancer: a Randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(6): 635-640.
[9] Yogesh Bendale, Vineeta Bendale, Saili Paul, Soumya Sundar Bhattacharyya. Green synthesis, characterization and anticancer potential of platinum nanoparticles Bioplatin. Journal of Chinese Integrative Medicine, 2012, 10(6): 681-689.
[10] Daniel A. Weber, Janelle M. Wheat , Geoffrey M. Currie. Cancer stem cells and the impact of Chinese herbs, isolates and other complementary medical botanicals: A review. Journal of Chinese Integrative Medicine, 2012, 10(5): 493-503.
[11] Surendra Kr. Sharma, Naveen Goyal. Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice. Journal of Chinese Integrative Medicine, 2012, 10(5): 555-560.
[12] Jing Guo, Ju-yong Wang, Zhan Zheng, Qing Wang, Chang-sheng Dong . Effects of Chinese herbal medicine Feiyanning Decoction on the ratio of CD4+CD25+ regulatory T cells and expression of transcription factor Foxp3 in mice bearing Lewis lung carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(5): 584-590.
[13] Qing-bo Lang, Dong-xia Zhai , Feng Huang, Jian-guo Chen, Yong-hui Zhang, Qun Liu, Xiao-feng Zhai , Bai Li, Chang-quan Ling. Investigation on traditional Chinese medicine syndrome distribution of 4 618 hepatitis B virus infection subjects in Qidong of Jiangsu Province, China. Journal of Chinese Integrative Medicine, 2012, 10(5): 525-531.
[14] Gang Guo, Jian-hua Xu, Jian-hong Han, Fang Liang, Yong Zhang, Qiang Zhang, Jue Sun, Zhong-ze Fan . Chinese herbal decoction Shiquan Dabu Tang inhibits tumor growth and angiogenesis of metastasis after primary tumor surgical removal in mice. Journal of Chinese Integrative Medicine, 2012, 10(4): 436-447.
[15] Meenakshi Sundaram, Satyajit Patra, Gunasingham Maniarasu. Antitumor activity of ethanol extract of Gracilaria edulis (Gmelin) Silva on Ehrlich ascites carcinoma-bearing mice. Journal of Chinese Integrative Medicine, 2012, 10(4): 430-435.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]