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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 661-664.doi: 10.3736/jcim20070612

• Original Experimental Research • Previous Articles     Next Articles

Effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing

Fu-Lun Li, Bin Li, Zhen-yi Wang, Bin Fan, Wen-bin Xu, Rong Xu   

  1. Department of Dermatology ,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine , of Traditional Chinese Medicine ,Shanghai 200437 ,China Shanghai University
  • Online:2007-11-20 Published:2007-11-15

Objective To study the effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing.Methods: Seventy-two male SD diabetic rats with full-thickness skin lesion were randomly divided into 3 groups: SJHYR 1-treated group, SJHYR 2-treated group and normal saline (NS) control group. SJHYR 1 was prepared with Shengji Recipe (SJR, a compound traditional Chinese herbal medicine for promoting granulation) and Huayu Recipe (HYR, a compound traditional Chinese herbal medicine for resolving stagnation) at a ratio of 1∶2, while SJHYR 2 was prepared with SJR and HYR at a ratio of 1∶1. Immunohistochemical method was used to assess Bax and Bcl-2 protein levels in granulation tissue.Results: SJHYR 1 could accelerate wound healing as compared with SJHYR 2 and NS (P<0.05). On the third day in experiment, Bax and Bcl-2 proteins were not found in any groups, but on the seventh and eleventh day in experiment, Bax and Bcl-2 proteins in SJHYR 1-treated group were much higher than those in the other two groups (P<0.05).Conclusion: SJR and HYR in different ratios may all have a role in regulating Bax and Bcl-2 expression in granulation tissue of diabetic rats during wound healing.

Key words: diabetes mellitus, ulcer, wound healing, Bcl-2 genes, rats

CLC Number: 

  • R587.29
[1] 肖秀丽, 王振宜, 范斌, 等 . 祛瘀生肌(内外合治) 法治疗慢性下肢皮肤溃疡50例临床观察[J]. 中国中西医结合皮肤性病学杂志, 2005,4(3):176-178
Xiao XL, Wang ZY, Fan B . et al. Clinical observation on stagnation-resolving and tissue-regenerating method in treatment of 50 cases of chronic lower-limb ulceration[J]. Zhongguo Zhong Xi Yi Jie He Pi Fu Xing Bing Xue Za Zhi, 2005,4(3):176-178
[2] 曹永清, 何春梅, 陆金根 . 温肾健脾方对大鼠慢性创面愈合的影响[J]. 中西医结合学报, 2005,3(3):220-224
Cao YQ, He CM, Lu JG . Effects of Wenshen Jianpi Recipe on chronic wound healing in rats[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(3):220-224
[3] Galkowska H, Olszewski WL, Wojewodzka U , et al. Expression of apoptosis- and cellcycle-related proteins in epidermis of venous leg and diabetic foot ulcers[J]. Surgery, 2003,134(2):213
[4] 付小兵, 孙同柱, 盛志勇 . 几种用于创伤修复研究的动物模型[J]. 中华实验外科杂志, 1999,16(5):479-480
Fu XB, Sun TZ, Sheng ZY . Some animal models for wound repair[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 1999,16(5):479-480
[5] 徐文彬, 李斌, 肖秀丽 , 等. 祛瘀生肌法对慢性下肢溃疡创面色泽及渗液量的影响[J]. 中国中西医结合外科杂志, 2006,12(2):103-105
Xu WB, Li B, Xiao XL , et al. Effects of stagnation-resolving and tissue-regenerating method on exudation of chronic lower-limb ulceration[J]. Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi, 2006,12(2):103-105
[6] 陈伟, 付小兵, 孙同柱 , 等. 皮肤溃疡伤口中Bax和Bcl-2蛋白含量的变化及其与溃疡发生的关系[J]. 现代康复, 2001,5(12):54-55
Chen W, Fu XB, Sun TZ , et al. Characteristics of Bax and Bcl-2 expression in ulcerative and normal skins and their effects on ulcer formation[J]. Xian Dai Kang Fu, 2001,5(12):54-55
[7] Sylvia CJ . The role of neutrophil apoptosis in influencing tissue repair[J]. J Wound Care, 2003,12(1):13-16
doi: 10.12968/jowc.2003.12.1.26458 pmid: 12572231
[8] Darby IA, Bisucci T, Hewitson TD , et al. Apoptosis is increased in a model of diabetes-impaired wound healing in genetically diabetic mice[J]. Int J Biochem Cell Biol, 1997,29(1):191-200
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