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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 409-413.doi: 10.3736/jcim20080416

• Original Experimental Research • Previous Articles     Next Articles

Duplication of an animal model of myocardial ischemia with blood stasis syndrome in mini-swines

Wen-yu Xu, Wei Wang(), Shu-zhen Guo, Tao Liu, Lei Liu, Yong-xin Yu   

  1. Department of Pharmacology, Basic Medical College, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2007-06-07 Online:2008-04-20 Published:2008-04-15
  • Contact: WANG Wei E-mail:wangwei26960@126.com

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Objective: To establish an animal model of myocardial ischemia with blood stasis syndrome in mini-swines.
Methods: An animal model of myocardial ischemia was established in mini-swines by oppressing the coronary artery through the expansion of inner layer of Ameroid constrictor and the Ameroid constrictor was implanted into the distal end of the initial part of the first branch of interventricular septum of ramus descendens anterior arteriae coronariae sinistrae. Dynamic observation of behavior changes, general health status and changes of hemorheological parameters in the mini-swines were made after operation.
Results: The coronary angiography showed that the stenosis rate in ischemic group was more than 75% four weeks after operation. Compared with before operation and sham-operated group, there were great changes of behavior, general health status, tongue color and hemorheological parameters in ischemic group (P<0.05).
Conclusion: The animal model of myocardial ischemia with blood stasis syndrome in mini-swines was established successfully 4 weeks after operation. The pathological process in the animal model is similar to that in the patients with chronic myocardial ischemia. So this model can be adopted in the research of myocardial ischemia with blood stasis syndrome.

Key words: myocardial ischemia, blood stasis, models, animal, minipigs

CLC Number: 

  • R542.2

Table 1

Ratios of red, blue and green of the tongue color before and after operation in pigs ($\bar{x}$±s)"

Group n Ratio of red Ratio of blue Ratio of green
Sham-operated
Before operation 10 0.44±0.03 0.26±0.03 0.30±0.02
2 weeks after operation 10 0.47±0.08 0.27±0.01 0.33±0.06
3 weeks after operation 10 0.48±0.04 0.26±0.02 0.26±0.02
4 weeks after operation 10 0.46±0.03 0.27±0.04 0.29±0.05
Ischemic
Before operation 9 0.52±0.09 0.24±0.01 0.24±0.08
2 weeks after operation 9 0.49±0.01 0.24±0.03 0.27±0.03
3 weeks after operation 9 0.42±0.04** 0.30±0.01**△ 0.29±0.04*
4 weeks after operation 9 0.43±0.02** 0.30±0.01** 0.27±0.02

Figure 1

Coronary angiography 4 weeks after operation in ischemic group A: Left coronary angiography; B: Right coronary angiography."

Figure 2

Coronary angiography 4 weeks after operation in sham-operated group A: Left coronary angiography; B: Right coronary angiography."

Table 2

Whole blood viscosity before and after operation ($\bar{x}$±s, mPa·s）"

Group n Whole blood viscosity
High (150/s) Middle (38/s) Low (10/s)
Sham-operated
Before operation 10 3.14±0.57 4.63±0.80 8.30±1.49
1 week after operation 10 4.23±0.43 6.47±0.50 10.93±0.63**
2 weeks after operation 10 3.97±0.74 5.63±0.75 9.62±0.65
3 weeks after operation 10 3.95±0.40 5.56±0.39 9.42±0.48
4 weeks after operation 10 4.06±0.41 5.89±0.55 10.31±1.09
Ischemic
Before operation 9 3.31±0.41 4.83±0.57 8.64±1.12
1 week after operation 9 4.37±0.36** 6.23±0.45** 10.68±0.89**
2 weeks after operation 9 4.03±0.63** 5.76±0.72** 9.91±0.93*
3 weeks after operation 9 4.42±0.54** 5.96±0.43** 9.96±0.38*
4 weeks after operation 9 3.82±0.65** 5.55±0.84** 9.75±1.46**

Table 3

Plasma viscosity and measured pressure plot before and after operation ($\bar{x}$±s)"

Group n Plasma viscosity (mPa·s) Measured pressure plot (%)
Sham-operated group
Before operation 10 1.54±0.39 42.33±4.41
1 week after operation 10 2.11±0.99 44.63±5.11
2 weeks after operation 10 1.87±0.10 42.00±2.65
3 weeks after operation 10 1.74±0.07 41.25±2.75
4 weeks after operation 10 2.01±0.28 41.33±2.31
Ischemic
Before operation 9 1.78±0.43 40.37±4.24
1 week after operation 9 2.29±0.54 44.50±2.08
2 weeks after operation 9 2.12±0.90 48.40±5.90**
3 weeks after operation 9 1.73±0.16 47.20±3.11**△
4 weeks after operation 9 1.82±0.34 43.88±5.56**

Table 4

Erythrocyte aggregation index, aggregation index integral area, deformation index and deformation index integral area before and after operation ($\bar{x}$±s）"

Group n Aggregation
index (%)		 Aggregation index
integral area		 Deformation
index (%)		 Deformation index
integral area
Sham-operated
Before operation 10 2.28±0.44 457.67±86.57 0.37±0.05 186.42±28.47
1 week after operation 10 2.69±0.47 519.44±53.48 0.32±0.06 173.07±14.23
2 weeks after operation 10 2.61±0.08 523.67±24.83 0.33±0.01 178.67±6.81
3 weeks after operation 10 2.70±0.21 519.00±64.59 0.35±0.05 186.00±34.24
4 weeks after operation 10 2.67±0.30 511.60±67.17 0.38±0.05 198.40±31.30
Ischemic
Before operation 9 2.33±0.45 460.52±88.59 0.38±0.06 195.85±31.16
1 week after operation 9 2.71±0.38 529.25±63.72 0.32±0.10 163.50±60.19
2 weeks after operation 9 2.45±0.19 468.40±45.51 0.36±0.06 170.80±41.51
3 weeks after operation 9 2.68±0.17 526.20±49.96 0.31±0.04* 154.80±30.11*
4 weeks after operation 9 2.50±0.37 492.08±72.34 0.38±0.04 187.60±31.10
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