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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 493-497.doi: 10.3736/jcim20080512

• Original Experimental Research • Previous Articles     Next Articles

Dilingdan Decoction prevents renal interstitial fibrosis in a rat model of unilateral ureteral obstruction

Hong-di Huan(), Jing-hong Zhang, Shi-rong Zheng, Wei-wei Meng, Jun-li Zhang   

  1. Department of Nephrology, the 85th Hospital of Peoples Liberation Army, Shanghai 200052, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15
  • Contact: HUAN Hong-di E-mail:huanpp@hotmail.com

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Objective: To investigate the preventive and therapeutic effects and the possible mechanisms of Dilingdan Decoction (DLDD), a compound Chinese herbal medicine, on rats with renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction (UUO).
Methods: Sixty SD rats were randomly divided into sham-operated group, untreated group, enalapril-treated group and DLDD-treated group. Blood urea nitrogen (BUN), serum creatinine (SCr), urine protein quantization in 24 hours and pathological changes of the obstructed kidney were observed. The expressions of transforming growth factor-β1 (TGF-β1), alpha-smooth muscle actin (α-SMA), fibronectin (FN) and laminin (LN) were detected by immunohistochemical method and colored-multimedia pathological image analysis system.
Results: Massive inflammatory infiltrates and collagen expression in renal interstitial in the untreated group were observed on the 7th day. Compared with the sham-operated group, percentages of area of TGF-β1, α-SMA, FN and LN expressions in the untreated group were markedly increased (P<0.05), while the percentage of area of interstitial fibrosis was decreased in the DLDD-treated group as compared with the untreated group (P<0.05). On the 14th day, the percentages of area of TGF-β1, α-SMA, FN and LN expressions were declined in two treated groups as compared with the untreated group (P<0.05), but had no statistical difference in biochemical indicators, including BUN, SCr and 24-hour urinary protein. On the 21st day, the level of SCr and the percentage of area of TGF-β1 expression in the DLDD-treated group were lower than those of the enalapril-treated group (P<0.05).
Conclusion: DLDD can reduce the excretion of urinary protein and the degree of interstitial fibrosis, and significantly inhibit the expressions of TGF-β1, α-SMA, FN and LN. DLDD is superior to enalapril in protecting renal function after long-time application in rats.

Key words: Dilingdan Decoction, renal interstitial fibrosis, transforming growth factor-betal, alpha-smooth muscle actin, fibronectin, laminin, rats

CLC Number: 

  • R692.6

Table1

Results of BUN, SCr, and urine protein quantization in 24 hours in different groups （$\bar{x}$±s）"

Group n BUN (mmol/L) SCr (mmol/L) 24 h urine protein (mg/24 h)
Sham-operated
Day 7 6 4.15±2.61 21.83±7.60 5.79±0.98
Untreated
Day 7 6 4.57±0.57 24.33±2.73 5.81±2.30
Day 14 6 5.48±0.20 41.33±5.47 12.74±3.55
Day 21 6 6.18±0.72 68.00±17.11 12.64±4.77
Enalapril-treated
Day 7 6 5.12±0.30 31.83±11.25 4.45±1.09
Day 14 6 5.58±0.44 55.50±26.85 11.02±3.48
Day 21 6 10.90±1.00 49.00±31.48△ 6.06±1.53△
DLDD-treated
Day 7 6 4.62±0.42 32.33±16.05 5.37±2.41
Day 14 6 6.30±1.15 50.67±4.97 9.52±7.96
Day 21 6 9.73±1.45 32.83±9.26△▲ 6.06±3.20△

Table 2

Percentages of interstitial fibrosis area in rats in different groups （$\bar{x}$±s, %）"

Group n Fibrosis area
Day 7 Day 14 Day 21
Sham-operated 6 0.66±0.49
Untreated 6 4.18±2.46* 3.06±1.03 4.87±0.71
Enalapril-treated 6 3.07±0.37* 1.46±0.61△ 2.26±1.47△
DLDD-treated 6 2.38±0.70*△ 2.34±0.79 1.85±0.45△▲

Table 3

Percentages of area of TGF-β1 and α-SMA expressions in renal interstitium in different groups （$\bar{x}$±s, %）"

Group n TGF-β1 α-SMA
Day 7 Day 14 Day 21 Day 7 Day 14 Day 21
Sham-operated 6 4.82±1.61 1.64±0.61
Untreated 6 11.09±2.70* 9.73±0.70 9.76±2.26 9.00±1.69 9.85±1.72 12.46±2.49
Enalapril-treated 6 8.08±1.88 5.37±2.77△ 3.35±1.52△ 5.63±1.67 7.31±1.78△ 7.41±1.72△
DLDD-treated 6 8.49±1.99 6.18±1.80△ 3.21±1.38△▲ 5.79±1.93 5.11±1.54△▲ 7.05±1.82△

Table 4

Percentages of area of FN and LN expressions in renal interstitium in different groups （$\bar{x}$±s, %）"

Group n FN LN
Day 7 Day 14 Day 21 Day 7 Day 14 Day 21
Sham-operated 6 4.82±1.02 2.13±0.81
Untreated 6 11.99±2.00* 13.16±1.99 14.46±0.73 10.20±2.23* 12.87±2.15 13.78±2.76
Enalapril-treated 6 8.09±1.88 6.30±1.02△ 8.04±2.13△ 7.19±1.19 8.91±2.85△ 9.78±2.01△
DLDD-treated 6 6.99±2.66 6.93±1.75△ 7.62±1.97△ 7.20±1.23 8.84±2.56△ 9.03±2.81△
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