肾阳虚大鼠模型的水通道蛋白1改变

doi:10.3736/jcim20080513

Abstract

Objective: To explore the material foundation of kidney-yang-deficiency syndrome.
Methods: Two kinds of rat models of deficiency of kidney-yang were induced by adenine intragastric administration (model Ⅰ) and hydrocortisone intramuscular injection (model Ⅱ). One hundred rats were randomly divided into normal control group, model Ⅰ group, low-dose model Ⅱ group, medium-dose model Ⅱ group and high-dose model Ⅱ group. After model establishment, contents of serum creatinine (SCr), blood urea nitrogen (BUN), and urine creatinine (UCr) were detected by automatic biochemistry analyzer; freezing point method was used for 24-hour urinary osmotic pressure (Uosm) testing and kaolinite method was used to detect the content of urinary 17-hydroxycorticosteroid (17-OHCS). Expression of aquaporin-1 in renal tissue was observed by using real time reverse transcription polymerase chain reaction.
Results: The rats of model groups had the characteristics of kidney-yang deficiency syndrome. The contents of SCr and BUN were significantly higher in model Ⅰgroup than in normal control group and three model Ⅱ groups (P<0.05), and UCr and Uosm were significantly lower (P<0.05). The level of urinary 17-OHCS and expression of aquaporin-1 in renal tissue were decreased in the model Ⅰ group (P<0.05) as compared with the normal control group. Compared with the normal control group, the content of SCr was increased and urinary 17-OHCS was decreased in the medium-dose model Ⅱ group, but the expression of aquaporin-1 was increased in three model Ⅱ groups with a dose-dependent manner.
Conclusion: Aquaporin-1 may be one of the material foundations of kidney-yang-deficiency syndrome.

Key words: deficiency of kidney-yang, aquaporin-1, rats

CLC Number:

R223.1+1

Yi Li, Li-qun He. Change of aquaporin-1 in rat models of kidney-yang-deficiency syndrome. Journal of Chinese Integrative Medicine, 2008, 6(5): 498-501.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20080513

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References 8

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Group	n	SCr (μmol/L)	BUN (mmol/L)
Normal control	20	29.25±2.77	6.17±0.31
Model Ⅰ	20	160.01±2.04*	40.10±0.44*
Low-dose model Ⅱ	20	34.06±1.04^△	8.21±0.71^△
Medium-dose model Ⅱ	20	34.80±4.63*^△	7.32±0.46^△
High-dose model Ⅱ	20	32.47±2.03^△	7.49±0.84^△

Group	n	UCr (μmol/L)	Uosm [mOsm/(kg·H2O)]	Urinary 17-OHCS (μmol/L)
Normal control	20	3 875.75±629.55	2 111.05±540.50	9.34±2.50
Model Ⅰ	20	46.23±59.61*	534.03±56.87*	5.07±2.04*
Low-dose model Ⅱ	20	3 770.80±2 721.40^△	1 936.30±196.81^△	8.77±6.00^△
Medium-dose model Ⅱ	20	3 383.45±2 410.63^△	1 940.25±473.45^△	6.22±4.78*
High-dose model Ⅱ	20	2 632.50±2 439.05^△	1 991.60±509.34^△	7.61±4.80^△

Group	n	AQP-1
Normal control	5	0.09±0.03
Model Ⅰ	5	0.05±0.04*
Low-dose model Ⅱ	5	0.13±0.09*^△
Medium-dose model Ⅱ	5	0.35±0.43*^△
High-dose model Ⅱ	5	2.48±2.05*^△

Change of aquaporin-1 in rat models of kidney-yang-deficiency syndrome

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