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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1049-1053.doi: 10.3736/jcim20081012

• Original Experimental Research • Previous Articles     Next Articles

Effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor and microvessel count in granulation tissue in rats with chronic skin ulcers

Hua-fa Que(), Jie Xing   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-04-10 Online:2008-10-20 Published:2008-10-15

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Objective: 

To explore the effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor (VEGF) and the microvessel count (MVC)in granulation tissue in rats with chronic skin ulcers.


Methods:

A total of 90 male SD rats with back full-thickness skin lesion were used in this study. Except for the normal control group, the rats were injected with hydrocortisone for inducing chronic skin ulcers, and were randomly divided into untreated group, Sijunzi Decoction group, Siwu Decoction group and Bazhen Decoction group. The time of wound healing was observed; immunohistochemical method and image analytical method were used to test the expressions of VEGF and MVC in granulation tissue of dermal chronic ulcers in the rats.


Results:

In the untreated group, the time of wound healing was significantly longer than that in the normal group (P<0.01). The time of wound healing was obviously shortened in the three treated groups as compared with the untreated group (P<0.01, P<0.05). In the Sijunzi Decoction group, the time of wound healing was obviously shorter than those in Siwu Decoction group and Bazhen Decoction group (P<0.05, P<0.01). In the untreated group, the expression of VEGF and MVC were significantly lower than those in the normal control group (P<0.01). The expression of VEGF and MVC were obviously increased in the three treated groups as compared with those in the untreated group (P<0.01, P<0.05). In the Sijunzi Decoction group, the expression of VEGF and MVC were obviously lower than those in Siwu Decoction group and Bazhen Decoction group (P<0.05, P<0.01).


Conclusion: 

Reinforcing qi and nourishing blood recipes can promote wound healing in rats through up-regulating the expression of VEGF in granulation tissue of dermal chronic ulcers, and inducing angiogenesis.

Key words: dermal chronic ulcer, wound healing, qi-blood reinforcing, vascular endothelial growth factor, rats

CLC Number: 

  • R753.7

Table 1

Days of wound healing of rats in different groups"

Group n Days of wound healing (d) Average time of wound healing (x±s, d)
Normal control 8 15-18 16.13±1.25
Untreated 8 19-23 20.75±1.39**
Sijunzi Decoction 8 14-18 16.25±1.04△△
Siwu Decoction 8 17-21 18.63±1.41*△▲
Bazhen Decoction 8 17-19 18.13±0.83*△△▲

Table 2

Expression of VEGF and MVC in granulation tissue of rats in different groups（x±s）"

Group n VEGF (mm2) MVC
Normal control 10 21.06±3.42 14.73±2.15
Untreated 10 10.46±1.40** 5.87±1.17**
Sijunzi Decoction 10 16.27±2.20**△△ 10.03±1.99**△△
Siwu Decoction 10 13.80±2.61**△△▲ 6.97±1.56**△△▲▲
Bazhen Decoction 10 12.73±1.92*△▲ 7.60±2.09**△△▲▲
[1] Fu XB, Wang DW. Contemporary wound repair[M]. Beijing: People's Military Medical Press,1999: 229-230
付小兵, 王德文 . 现代创伤修复学[M]. 北京: 人民军医出版社,1999: 229-230
[2] Que HF, Tang HJ, Wang LY , et al. Study on wound healing mechanism of replenishing qi and dissipating stagnation therapy for chronic skin ulcers[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(3):243-246
阙华发, 唐汉钧, 王林扬 , 等. 益气化瘀法促进慢性难愈性创面修复愈合的机制研究[J]. 中西医结合学报, 2005,3(3):243-246
[3] Galeano M, Deodato B, Altavilla D , et al. Effect of recombinant adeno-associated virus vector-mediated vascu1ar endothelial growth factor gene transfer on wound healing after burn injury[J]. Crit Care Med, 2003,31(4):1017-1025
doi: 10.1097/01.CCM.0000059435.88283.C2
[4] Pierce GF, Tarpley JE, Allman RM . Tissue repair processes in healing chronic pressure ulcers treated with recombinant platelet-derived growth factor BB[J]. Am J Pathol, 1994,145(6):1399-1410
[5] Tuan TL, Nichter LS . The molecular basis of keloid and hypertrophic scar formation[J]. Mol Med Today, 1998,4(1):19-24
doi: 10.1016/S1357-4310(97)80541-2 pmid: 9494966
[6] Dvorak HK . Angiogenesis: update 2005[J]. J Thromb Haemost, 2005,3(8):1835-1842
doi: 10.1111/jth.2005.3.issue-8
[7] Xu WR, Wang L, Zhao HF , et al. Application of human vascular endothelial growth factor gene-modified engineering membrane in promoting rat wound healing[J]. Wai Ke Li Lun Yu Shi Jian, 2006,11(3):244-247
doi: 10.3969/j.issn.1007-9610.2006.03.020
许伟榕, 王莉, 赵涵芳 , 等. 人血管内皮生长因子基因工程生物膜愈合大鼠皮肤创伤的初步研究[J]. 外科理论与实践, 2006,11(3):244-247
doi: 10.3969/j.issn.1007-9610.2006.03.020
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