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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (4): 400-400.doi: 10.3736/jcim20090411

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Effects of Caulis Sargentodoxae Granule on expressions of vascular endothelial growth factor and its receptor-2 in rats with endometriosis

 Yang Caoa,Ting-ting Zhangb,Shu-wu Xiec,Yan Zhuc,Lan-di Shua,Zhi-li Songa,Lin Caob,De-ying Daia   

  1. a Department of Gynecology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
    b Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    c Department of Reproductive Pharmacology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
  • Received:2008-09-21 Accepted:2008-12-02 Online:2009-04-20 Published:2009-04-15

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Objective

To investigate the effects of Caulis Sargentodoxae Granule (CSG), a compound traditional Chinese herbal medicine for treating endometriosis, on expressions of vascular endothelial growth factor (VEGF) and its receptor-2 fetal liver kinase-1 (Flk-1) in rats with endometriosis.
Methods

A rat model of endometriosis was established by autotransplant of endometria. Forty-eight endometriosis rats were randomly divided into 6 groups: untreated group, castrate group, mifepristone group (5 mg/kg) and low- (20 g/kg), medium- (40 g/kg) and high-dose (80 g/kg) CSG group. After 21-day consecutive treatment, the expressions of VEGF and its receptor Flk-1 in ectopic endometria were detected by immunohistochemical method, and the volume of the ectopic endometria was also measured by using electronic digital calipers.
Results

Compared with the untreated group, the endometrial volumes in the treated groups were significantly decreased (P<0.05) except for the low-dose CSG group. Histopathologic observation showed that the epithelial layer of ectopic endometrium was less and the thickness of epithelial layer was thinner than those in the untreated group (P<0.01), but the low-dose CSG group had few changes. The VEGF and its receptor Flk-1 expressed abundantly in the cytoplasm of the glandular epithelium and vascular endothelial cell in all the groups. Compared with the untreated group, the expressions of VEGF and Flk-1 of ectopic endometrium in the CSG-treated groups, the mifepristone group and the castrate group were significantly decreased (P<0.05, P<0.01).
Conclusion

CSG can treat the endometriosis in rats, and its action may be associated with decreasing the expressions of VEGF and its receptor Flk-1 in ectopic endometrium.

Key words: Caul is Sargentodoxae Granules, Endometriosis, Vascular endothelial growth factor, Vascular endothelial growth factor receptor-2, Rats

Table 1

Effects of CSG on the volumes of ectopic endometria in rats[Median (Q1, Q3)]"

Group n Volume of ectopic endometrium (mm3) Inhibition rate (%)
Before treated After treated
Untreated 8 71.958 (52.504, 89.750) 89.012 (49.598, 235.497) –21.582(–310.009, 20.914)
Castrate 8 73.215 (43.896, 130.233) 1.769 (0.001, 23.977)* 99.211 (65.506, 99.996)*
Mifepristone 8 105.096 (84.601, 115.745) 36.340 (25.642, 48.712)* 60.791 (40.695, 67.034)*
Low-dose CSG 8 51.703 (44.215, 66.761) 33.242 (24.758, 53.559) 49.471 (–40.899, 69.698)
Medium-dose CSG 8 65.582 (40.893, 86.315) 24.104 (17.723, 36.332)* 54.407 (20.225, 75.781)*
High-dose CSG 8 64.529 (54.367, 109.411) 18.439 (8.967, 36.464)* 75.759 (56.734, 85.050)*

Table 2

Effects of CSG on the thickness of ectopic endometria in rats (x±s, μm)"

Group n Thickness of ectopic endometrium
Untreated 8 317.44±63.38
Castrate 8 112.39±19.09**
Mifepristone 8 182.50±23.06**
Low-dose CSG 8 272.50±62.78
Medium-dose CSG 8 200.46±32.89**
High-dose CSG 8 128.20±38.39**

Figure 1

Ectopic endometrium thickness observed by light microscope (HE staining, ×100) A: Untreated group; B: Castrate group; C: Mifepristone group; D: Low-dose CSG group; E: Medium-dose CSG group; F: High-dose CSG group. The arrows point to the glandular epithelial cell of ectopic endometrium tissue."

Table 3

Effects of CSG on the VEGF and Flk-1 expressions in ectopic endometrium in rats (x±s)"

Group n VEGF Flk-1
Untreated 8 90.81±11.45 98.78±8.56
Castrate 8 112.08±4.10** 124.22±4.20**
Mifepristone 8 106.67±1.37* 123.97±15.44**
Low-dose CSG 8 97.69±4.48 105.94±8.19
Medium-dose CSG 8 102.64±2.80* 115.00±12.28*
High-dose CSG 8 109.06±2.73** 122.86±21.22*

Figure 2

Expression of VEGF in the ectopic endometrium observed by light microscope (SABC staining, ×400) A: Untreated group; B: Castrate group; C: Mifepristone group; D: Low-dose CSG group; E: Medium-dose CSG group; F: High-dose CSG group. The arrows point to the expression of VEGF in ectopic endometrium tissue."

Figure 3

Expression of Flk-1 in the ectopic endometrium observed by light microscope (SABC staining, ×400) A: Untreated group; B: Castrate group; C: Mifepristone group; D: Low-dose CSG group; E: Medium-dose CSG group; F: High-dose CSG group. The arrows point to the expression of Flk-1 in ectopic endometrium tissue."

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