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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (10): 958-962.doi: 10.3736/jcim20091009

• Original Experimental Research • Previous Articles     Next Articles

Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization

Jian-ye Yuana,b,Jian-qun Xieb,Da-zheng Wua,Yu Zhengb,Xiang-xue Panc,Xiao-yan Feib,Hai-zhen Xub   

  1. a Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China
    c Department of Gastroenterology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
  • Received:2009-05-31 Accepted:2009-08-20 Online:2009-10-20 Published:2009-10-15
  • Contact: Jian-qun Xie E-mail:xiejq@gmail.com

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Objective

To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.
Methods

By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca2+ of internal calcium store were assessed respectively.
Results

Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca 2+of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase. Conclusion: Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca 2+, which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca 2+from calcium store.

Key words: Tongxie Yaofang, Colon, Smooth muscle, Calcium mobilization, Rats

Figure 1

Inhibitory effects of Tongxie Yaofangon contraction of muscle strips induced by KCl Data were represented as $\bar{x}$±s. n=6. **P<0.01, vs control."

Figure 2

Inhibitory effects of Tongxie Yaofang on contraction of muscle strips induced by exhausting Ca2+ of internal calcium store Data were represented as $\bar{x}$±s. n=6. **P<0.01, vs control."

Table 1

Effects of Tongxie Yaofang on two phases of the contraction induced by Ach ($\bar{x}$±s, %)"

Group n Percent tension of the first contractile phase Percent tension of the second contractile phase
Krebs 6 48.81±5.85 111.28±5.74
300 μg/mL Tongxie Yaofang 6 50.49±7.15 101.76±4.31
1 000 μg/mL Tongxie Yaofang 6 56.41±6.18 91.60±3.63
3 000 μg/mL Tongxie Yaofang 6 47.12±4.97 61.47±7.38**
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