Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (10): 958-962.doi: 10.3736/jcim20091009

• Original Experimental Research • Previous Articles     Next Articles

Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization

Jian-ye Yuana,b,Jian-qun Xieb,Da-zheng Wua,Yu Zhengb,Xiang-xue Panc,Xiao-yan Feib,Hai-zhen Xub   

  1. a Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China
    c Department of Gastroenterology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China
  • Received:2009-05-31 Accepted:2009-08-20 Online:2009-10-20 Published:2009-10-15
  • Contact: Jian-qun Xie E-mail:xiejq@gmail.com

Objective

To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.
Methods

By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca2+ of internal calcium store were assessed respectively.
Results

Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca 2+of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase. Conclusion: Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca 2+, which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca 2+from calcium store.

Key words: Tongxie Yaofang, Colon, Smooth muscle, Calcium mobilization, Rats

Figure 1

Inhibitory effects of Tongxie Yaofangon contraction of muscle strips induced by KCl Data were represented as $\bar{x}$±s. n=6. **P<0.01, vs control."

Figure 2

Inhibitory effects of Tongxie Yaofang on contraction of muscle strips induced by exhausting Ca2+ of internal calcium store Data were represented as $\bar{x}$±s. n=6. **P<0.01, vs control."

Table 1

Effects of Tongxie Yaofang on two phases of the contraction induced by Ach ($\bar{x}$±s, %)"

Group n Percent tension of the first contractile phase Percent tension of the second contractile phase
Krebs 6 48.81±5.85 111.28±5.74
300 μg/mL Tongxie Yaofang 6 50.49±7.15 101.76±4.31
1 000 μg/mL Tongxie Yaofang 6 56.41±6.18 91.60±3.63
3 000 μg/mL Tongxie Yaofang 6 47.12±4.97 61.47±7.38**
[1] Chu GW . Textual research on Tongxie Yaofang[J]. Zhong Yi Yao Xue Kan, 2006,24(11):2036-2037
楚更五 . 痛泻要方考释[J]. 中医药学刊, 2006,24(11):2036-2037
[2] Fang P . Clinical effect of Jiaweitongxie prescription in treating irritable bowel syndrome[J]. Zhejiang Zhong Xi Yi Jie He Za Zhi, 2008,18(8):609-670
doi: 10.3969/j.issn.1005-4561.2008.08.004
方萍 . 加味痛泻要方治疗肠易激综合征疗效观察[J]. 浙江中西医结合杂志, 2008,18(8):609-670
doi: 10.3969/j.issn.1005-4561.2008.08.004
[3] Wu YN, Huang DH . Clinical observation of Tongxie Yaofang in treatment of patients with diarrhea-type irritable bowel syndrome[J]. Liaoning Zhong Yi Yao Da Xue Xue Bao, 2008,10(6):94-95
doi: 10.3969/j.issn.1673-842X.2008.06.054
吴耀南, 黄墩煌 . 痛泻要方加味治疗腹泻型肠易激综合征临床观察[J]. 辽宁中医药大学学报, 2008,10(6):94-95
doi: 10.3969/j.issn.1673-842X.2008.06.054
[4] Wang BB, Chu GW, Zheng M . Effect of Tongxie Yaofang and acetylcholine on the smooth muscle isolated from the small intestine in rabbit[J]. Zhejiang Zhong Yi Za Zhi, 2007,42(4):235-237
doi: 10.3969/j.issn.0411-8421.2007.04.038
王蓓蓓, 楚更五, 郑梅 . 痛泻要方与乙酰胆碱对家兔离体小肠平滑肌作用的研究[J]. 浙江中医杂志, 2007,42(4):235-237
doi: 10.3969/j.issn.0411-8421.2007.04.038
[5] Chu GW, Zhang JY, Rao GX, Li P, Yang Y, Liang L, Nie J . Effects of the water extracts and its remnant components of Tongxieyaofang decoction on the motility of intestinal muscle in rats[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2007,15(3):153-155
doi: 10.3969/j.issn.1671-038X.2007.03.005
楚更五, 张建英, 饶高雄, 李平, 杨云, 梁玲, 聂坚 . 痛泻要方水提取物及其萃取成分对大鼠体外肠肌的作用[J]. 中国中西医结合消化杂志, 2007,15(3):153-155
doi: 10.3969/j.issn.1671-038X.2007.03.005
[6] Myers BS, Martin JS, Dempsey DT, Parkman HP, Thomas RM, Ryan JP . Acute experimental colitis decreases colonic circular smooth muscle contractility in rats[J]. Am J Physiol, 1997,273(4 Pt 1):G928-G936
doi: 10.1152/ajpcell.1997.273.3.C928
[7] Dai Y, Liu JX, Li JX, Xu YF . Effect of pinaverium bromide on stress-induced colonic smooth muscle contractility disorder in rats[J]. World J Gastroenterol, 2003,9(3):557-561
doi: 10.3748/wjg.v9.i3.557
[8] Ke DP, Zhou H, Li ZW, Kong DH, Ma R . Capacitative Ca2+ entry mediates contraction in rat distal colon smooth muscle[J]. Zhongguo Yao Li Xue Tong Bao, 2006,22(3):344-348
柯道平, 周华, 李忠稳, 孔德虎, 马嵘 . 库容性Ca2+内流介导大鼠远端结肠平滑肌收缩[J]. 中国药理学通报, 2006,22(3):344-348
[9] Yang BF. Pharmacology on ion channel[M]. Beijing: People's Medical Publishing House,2005:155, 161, 172
杨宝峰 . 离子通道药理学[M]. 北京: 人民卫生出版社,2005:155, 161, 172
[10] Kinoshita K, Sato K, Hori M, Ozaki H, Karaki H . Decrease in activity of smooth muscle L-type Ca2+ channels and its reversal by NF-kappaB inhibitors in Crohn's colitis model[J]. Am J Physiol Gastrointest Liver Physiol, 2003,285(3):G483-G493
doi: 10.1152/ajpgi.00038.2003
[11] McCarron JG, Craig JW, Bradley KN, Muir TC . Agonist-induced phasic and tonic responses in smooth muscle are mediated by InsP3[J]. J Cell Sci, 2002,115(Pt 10):2207-2218
[12] Yu ZP, Li XF, Li WJ, He XS, Lin Q . Effects and mechanism of extracting G2 from Gastrodia Elata Blume on relaxation of isolated ileum smooth muscle[J]. Yunnan Zhong Yi Xue Yuan Xue Bao, 2005,28(3):15-18
淤泽溥, 李秀芳, 李文军, 何晓山, 林青 . 天麻提取物对回肠平滑肌的舒张作用及其机制研究[J]. 云南中医学院学报, 2005,28(3):15-18
[13] Zhang WW, Li Y, Wang XQ, Tian F, Cao H, Wang MW, Sun QS . Effects of magnolol and honokiol derived from traditional Chinese herbal remedies on gastrointestinal movement[J]. World J Gastroenterol, 2005,11(28):4414-4418
doi: 10.3748/wjg.v11.i28.4414
[14] Karaki H, Ozaki H, Hori M, Mitsui-Saito M, Amano K, Harada K, Miyamoto S, Nakazawa H, Won KJ, Sato K . Calcium movements, distribution and functions in smooth muscle[J]. Pharmacol Rev, 1997,49(2):157-230
[15] Yuan JY, Xie JQ . Inhibitory effect of extract from Shuganyin on contractility of rats' colonic smooth muscles[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2007,21(1):50-53
doi: 10.3969/j.issn.1008-861X.2007.01.018
袁建业, 谢建群 . 疏肝饮提取物对大鼠结肠平滑肌收缩的抑制作用[J]. 上海中医药大学学报, 2007,21(1):50-53
doi: 10.3969/j.issn.1008-861X.2007.01.018
[1] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[2] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[3] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[4] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[5] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[6] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[7] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[8] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[9] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[10] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[11] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[12] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[13] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[14] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
[15] Manjusha Choudhary​, Vipin Kumar​, Surender Singh​. Gastric antisecretory and cytoprotective effects of hydroalcoholic extracts of Plumeria alba Linn. leaves in rats. Journal of Integrative Medicine, 2014, 12(1): 42-51.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[4] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[5] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[8] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[9] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[10] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052