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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 244-249.doi: 10.3736/jcim20100308

• Original Experimental Research • Previous Articles     Next Articles

Effects of eye drops of Buddleja officinalis Maxim. extract on lacrimal gland cell apoptosis in castrated rats with dry eye

Qing-hua Peng,Xiao-lei Yao, Quan-long Wu, Han-yu Tan, Jing-rong Zhang   

  1. Key Department of Chinese Traditional Ophthalmology, the First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
  • Received:2009-09-04 Accepted:2010-02-05 Online:2010-03-20 Published:2010-03-15
  • Contact: Qing-hua Peng,Xiao-lei Yao E-mail:pqhz_520@163.com;yxlshh@126.com

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Objective

To explore the possible mechanism of eye drops of Buddleja officinalis extract in treating dry eye of castrated rats by analyzing the expressions of Bax and Bcl-2 proteins.
Methods

Forty-five Wistar male rats were randomly divided into sham-operated group, untreated group and eye drops of Buddleja officinalis Maxim. extract (treatment) group. The dry eye model was established with orchiectomy in the untreated group and treatment group. Rats in the treatment group were treated with eye drops of Buddleja officinalis Maxim. extract, one drop once, three times daily. Eyes of rats in the sham-operated group and untreated group were instilled with normal saline. After one-, two-, or three-month treatment, five rats in each group were scarified respectively. Then samples were taken to detect related indices. Expressions of Bax and Bcl-2 of lacrimal gland were checked by immunohistochemical method and quantity of apoptotic cells was counted.
Results

After one-, two- or three-month treatment, the quantities of expressions of Bax in acinar epithelial cells and glandular tube cells were significantly lower, and those of Bcl-2 were significantly higher in the treatment group than in the untreated group, and the quantities of apoptotic cells of the treatment group were significantly lower than those of the untreated group (P<0.01).
Conclusion

The main components of extract of Buddleja officinalis Maxim. are flavonoids, which can significantly inhibit cell apoptosis in lacrimal gland.

Key words: Orchiectomy, Xerophthalmia, Lacrimal apparatus, Apoptosis, Buddleja officinalis, Eye drops, Rats

"

Group n Bax Bcl-2 Quantity of apoptotic cells
Sham-operated
After 1-month treatment 5 0.125±0.044 0.314±0.047 11.34±4.67
After 2-month treatment 5 0.164±0.044 0.324±0.034 12.01±5.55
After 3-month treatment 5 0.146±0.038 0.363±0.094 11.87±3.22
Untreated
After 1-month treatment 5 0.641±0.134△△ 0.274±0.074 45.45±9.27△△
After 2-month treatment 5 0.761±0.084△△ 0.245±0.055 58.23±10.32△△
After 3-month treatment 5 0.796±0.036△△ 0.232±0.021 70.41±11.68*△△
Treatment
After 1-month treatment 5 0.241±0.091▲▲ 0.505±0.114▲▲ 12.77±7.91▲▲
After 2-month treatment 5 0.266±0.158▲▲ 0.495±0.024▲▲ 21.27±8.89▲▲
After 3-month treatment 5 0.344±0.073▲▲ 0.525±0.065▲▲ 31.89±6.67▲▲

Figure 1

Expression of Bax protein in lacrimal gland in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Sham-operated group after 1-month treatment; B: Sham-operated group after 2-month treatment; C: Sham-operated group after 3-month treatment; D: Untreated group after 1-month treatment; E: Untreated group after 2-month treatment; F: Untreated group after 3-month treatment; G: Treatment group after 1-month treatment; H: Treatment group after 2-month treatment; I: Treatment group after 3-month treatment."

Figure 2

Expression of Bcl-2 protein in lacrimal gland in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Sham-operated group after 1-month treatment; B: Sham-operated group after 2-month treatment; C: Sham-operated group after 3-month treatment; D: Untreated group after 1-month treatment; E: Untreated group after 2-month treatment; F: Untreated group after 3-month treatment; G: Treatment group after 1-month treatment; H: Treatment group after 2-month treatment; I: Treatment group after 3-month treatment."

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