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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 250-255.doi: 10.3736/jcim20100309

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xiaotan Sanjie Decoction-containing serum on proliferation and apoptosis of human gastric cancer cells MKN-45

Mu-wei Gui, I Pin-kang Wei,Ye Lu, Wei Guo, Zhi-feng Qin, Da-zhi Sun   

  1. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Received:2009-10-29 Accepted:2009-01-10 Online:2010-03-20 Published:2010-03-15
  • Contact: Pin-kang Wei E-mail:czzyk@smmu.edu.cn

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Objective

To observe the effects of serum containing Xiaotan Sanjie Decoction, a compound traditional Chinese herbal medicine, on proliferation and apoptosis of human gastric cancer cell line MKN-45.
Methods

After coculturing MKN-45 cells with low-, medium- and high-dose Xiaotan Sanjie Decoction-containing serum, inverted microscope was utilized to observe morphological changes and counting chamber was used to count the MKN-45 cells; the proliferation of MKN-45 cells was determined by cell counting kit 8; Annexin V-fluorescein isothiocyanate/propidium iodide double label method was used to detect apoptosis rate of MKN-45 cells.
Results

Xiaotan Sanjie Decoction-containing serum significantly inhibited the proliferation of MKN-45 cells. The typical morphological changes of apoptotic MKN-45 cells were observed after treating with Xiaotan Sanjie Decoction-containing serum. The cell apoptosis was also observed by flow cytometry and the apoptosis rates of medium- and high-dose Xiaotan Sanjie Decoction-containing serum groups were higher than that of low-dose group.
Conclusion

Xiaotan Sanjie Decoction-containing serum can inhibit the MKN-45 cell proliferation and induce cell apoptosis, which provides an experimental evidence for its treatment of human gastric cancer.

Key words: Compound (TCD), Gastric tumor, Serum pharmacology, Apoptosis, Proliferation

Table 1

Effects of Xiaotan Sanjie Decoction-containing serum on proliferation of MKN-45 cell line"

Group n OD (x±s) Proliferation rate (%)
Control
24 h 5 0.110 6±0.003 6 100
48 h 5 0.118 6±0.0036 100
72 h 5 0.162 6±0.004 5 100
96 h 5 0.221 0±0.004 3 100
Low-dose
24 h 5 0.109 0±0.004 2 98.55
48 h 5 0.116 8±0.003 7 98.48
72 h 5 0.156 0±0.001 6* 95.94
96 h 5 0.209 2±0.006 1* 94.66
Medium-dose
24 h 5 0.106 4±0.004 6 96.20
48 h 5 0.112 6±0.004 0*△ 94.94
72 h 5 0.122 0±0.002 9*△ 75.03
96 h 5 0.188 4±0.006 0*△ 85.25
High-dose
24 h 5 0.105 4±0.004 6 95.30
48 h 5 0.112 4±0.004 2*△ 94.77
72 h 5 0.125 2±0.003 3*△ 77.00
96 h 5 0.189 2±0.004 4*△ 85.61

Figure 1

Effects of Xiaotan Sanjie Decoction-containing serum on growth of MKN-45 cells"

Figure 2

Effects of Xiaotan Sanjie Decoction-containing serum on morphological changes of MKN-45 cells (Inverted microscopy, ×100)A: Normal cells; B: Control group; C: Low-dose group; D: Medium-dose group; E: High-dose group."

Figure 3

Effects of Xiaotan Sanjie Decoction-containing serum on apoptosis of MKN-45 cells"

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