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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 256-262.doi: 10.3736/jcim20100310

• Original Experimental Research • Previous Articles     Next Articles

Influence of Aloe polysaccharide on proliferation and hyaluronic acid and hydroxyproline secretion of human fibroblasts in vitro

Ling-ying Liua, Xiao-dong Chenb,Bo-yu Wua, Qiong Jianga   

  1. a Provincial Burns Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China
    b Burns Institute, the First Affiliated Hospital of General Hospital of the Peopled Liberation Army, Beijing 100048, China
  • Received:2009-11-05 Accepted:2010-01-17 Online:2010-03-20 Published:2010-03-15


To investigate the effect of Aloe polysaccharide on proliferation and hyaluronic acid and hydroxyproline secretion of human fibroblasts in vitro.

The fibroblasts were treated with different doses of polysaccharide (0, 25, 50, 100, 200, 400 mg/L). Subsequently, cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell cycle by flow cytometry, evaluation of the Aloe polysaccharide toxic effect by acridine orange-ethidium bromide staining, evaluation of the cell injury by lactate dehydrogenase (LDH) assay, and the collagen synthesis by 3H-proline incorporation. In addition, hyaluronic acid and hydroxyproline levels in the supernatants of cultured fibroblasts were measured by enzyme-linked immunosorbent assay.

The proliferation of fibroblasts was induced with polysaccharide in a dose-dependent manner, reaching its highest level on 5th day. Meanwhile, the percentage of cells at phase G0/G1 was decreased, while that at phases G2/M and S was increased significantly in Aloe polysaccharide-treated groups as compared with those in the control group (P<0.05). Additionally, the apoptosis of the fibroblasts showed no differences among all groups. The collagen synthesis was increased and cell injury decreased in polysaccharide-treated groups as compared with those in control group (P<0.05), while the levels of hyaluronic acid and hydroxyproline in the supernatants of fibroblasts treated with polysaccharide were significantly higher than those in the control group (P<0.05).

The Aloe polysaccharide promotes both the proliferation of fibroblasts and the production of hyaluronic acid and hydroxyproline in fibroblasts. This indicates that the Aloe polysaccharide may play an important role in the extracellular matrix remodeling during wound healing.

Key words: Aloe polysaccharides, Fibroblasts, Hyaluronic acid, Hydroxyproline

Figure 1

Effects of different concentrations of polysaccharide from Aloe on fibroblast proliferation"

Table 1

Effects of polysaccharide from Aloe on cell cycle in fibroblasts"

Group n G0/G1 (%) G2/M+S (%) P<0.01
Control group 1 000 32.3 67.7
25 mg/L Aloe polysaccharide 1 000 26.5** 73.4** vs control group
50 mg/L Aloe polysaccharide 1 000 26.1** 74.0** vs control group
100 mg/L Aloe polysaccharide 1 000 28.6** 71.3** vs control group
200 mg/L Aloe polysaccharide 1 000 22.5** 77.5** vs control group
400 mg/L Aloe polysaccharide 1 000 21.2** 78.9** vs control group

Figure 2

Effects of polysaccharide from Aloe on cell cycle in fibroblastsA: Control group; B: 25 mg/L Aloe polysaccharide group; C: 50 mg/L Aloe polysaccharide group; D: 100 mg/L Aloe polysaccharide group; E: 200 mg/L Aloe polysaccharide group; F: 400 mg/L Aloe polysaccharide group."

Figure 3

Effects of polysaccharide from Aloe on apoptosis of fibroblastsA: Control group; B: 50 mg/L Aloe polysaccharide group; C: 400 mg/L Aloe polysaccharide group."

Table 2

Effects of Aloe polysaccharide on LDH leakage rate and 3H-proline incorporation in fibroblasts ($\bar{x}±s$)"

Group n LDH leakage rate (%) 3H-proline incorporation (c/min)
Control 6 25.03±8.78 1 585.67±352.19
25 mg/L Aloe polysaccharide 6 23.77±5.33 2 608.17±670.95**
50 mg/L Aloe polysaccharide 6 20.90±5.92 2 693.00±772.40**
100 mg/L Aloe polysaccharide 6 20.83±7.15 2 773.67±796.15**
200 mg/L Aloe polysaccharide 6 19.24±4.65 2 985.83±445.12**
400 mg/L Aloe polysaccharide 6 18.49±6.39 3 664.33±836.88**

Table 3

Effects of Aloe polysaccharide on hyaluronic acid and hydroxyproline secretion of fibroblasts ($\bar{x}±s$, mg/L)"

Group n Hyaluronic acid Hydroxyproline
Control 8 349.22±267.43 9.16±1.02
25 mg/L Aloe polysaccharide 8 431.41±254.14 12.46±2.38**
50 mg/L Aloe polysaccharide 8 1 118.54±372.39** 15.31±5.15**
100 mg/L Aloe polysaccharide 8 994.08±470.80** 17.37±14.69
200 mg/L Aloe polysaccharide 8 838.99±710.89 18.36±14.88
400 mg/L Aloe polysaccharide 8 1 627.11±1 086.56** 26.90±21.60*
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