消痰散结方药物血清对人胃癌MKN-45细胞增殖和凋亡的影响

doi:10.3736/jcim20100309

Abstract

Objective

To observe the effects of serum containing Xiaotan Sanjie Decoction, a compound traditional Chinese herbal medicine, on proliferation and apoptosis of human gastric cancer cell line MKN-45.
Methods

After coculturing MKN-45 cells with low-, medium- and high-dose Xiaotan Sanjie Decoction-containing serum, inverted microscope was utilized to observe morphological changes and counting chamber was used to count the MKN-45 cells; the proliferation of MKN-45 cells was determined by cell counting kit 8; Annexin V-fluorescein isothiocyanate/propidium iodide double label method was used to detect apoptosis rate of MKN-45 cells.
Results

Xiaotan Sanjie Decoction-containing serum significantly inhibited the proliferation of MKN-45 cells. The typical morphological changes of apoptotic MKN-45 cells were observed after treating with Xiaotan Sanjie Decoction-containing serum. The cell apoptosis was also observed by flow cytometry and the apoptosis rates of medium- and high-dose Xiaotan Sanjie Decoction-containing serum groups were higher than that of low-dose group.
Conclusion

Xiaotan Sanjie Decoction-containing serum can inhibit the MKN-45 cell proliferation and induce cell apoptosis, which provides an experimental evidence for its treatment of human gastric cancer.

Key words: Compound (TCD), Gastric tumor, Serum pharmacology, Apoptosis, Proliferation

Mu-wei Gui, Pin-kang Wei, Ye Lu, Wei Guo, Zhi-feng Qin, Da-zhi Sun. Effects of Xiaotan Sanjie Decoction-containing serum on proliferation and apoptosis of human gastric cancer cells MKN-45. Journal of Chinese Integrative Medicine, 2010, 8(3): 250-255.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20100309

http://www.jcimjournal.com/EN/Y2010/V8/I3/250

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Figures/Tables 4

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References 11

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Group	n	OD (x±s)	Proliferation rate (%)
Control
24 h	5	0.110 6±0.003 6	100
48 h	5	0.118 6±0.0036	100
72 h	5	0.162 6±0.004 5	100
96 h	5	0.221 0±0.004 3	100
Low-dose
24 h	5	0.109 0±0.004 2	98.55
48 h	5	0.116 8±0.003 7	98.48
72 h	5	0.156 0±0.001 6*	95.94
96 h	5	0.209 2±0.006 1*	94.66
Medium-dose
24 h	5	0.106 4±0.004 6	96.20
48 h	5	0.112 6±0.004 0*^△	94.94
72 h	5	0.122 0±0.002 9*^△	75.03
96 h	5	0.188 4±0.006 0*^△	85.25
High-dose
24 h	5	0.105 4±0.004 6	95.30
48 h	5	0.112 4±0.004 2*^△	94.77
72 h	5	0.125 2±0.003 3*^△	77.00
96 h	5	0.189 2±0.004 4*^△	85.61

Effects of Xiaotan Sanjie Decoction-containing serum on proliferation and apoptosis of human gastric cancer cells MKN-45

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