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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 250-255.doi: 10.3736/jcim20100309

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xiaotan Sanjie Decoction-containing serum on proliferation and apoptosis of human gastric cancer cells MKN-45

Mu-wei Gui, I Pin-kang Wei,Ye Lu, Wei Guo, Zhi-feng Qin, Da-zhi Sun   

  1. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Received:2009-10-29 Accepted:2009-01-10 Online:2010-03-20 Published:2010-03-15
  • Contact: Pin-kang Wei E-mail:czzyk@smmu.edu.cn

Objective

To observe the effects of serum containing Xiaotan Sanjie Decoction, a compound traditional Chinese herbal medicine, on proliferation and apoptosis of human gastric cancer cell line MKN-45.
Methods

After coculturing MKN-45 cells with low-, medium- and high-dose Xiaotan Sanjie Decoction-containing serum, inverted microscope was utilized to observe morphological changes and counting chamber was used to count the MKN-45 cells; the proliferation of MKN-45 cells was determined by cell counting kit 8; Annexin V-fluorescein isothiocyanate/propidium iodide double label method was used to detect apoptosis rate of MKN-45 cells.
Results

Xiaotan Sanjie Decoction-containing serum significantly inhibited the proliferation of MKN-45 cells. The typical morphological changes of apoptotic MKN-45 cells were observed after treating with Xiaotan Sanjie Decoction-containing serum. The cell apoptosis was also observed by flow cytometry and the apoptosis rates of medium- and high-dose Xiaotan Sanjie Decoction-containing serum groups were higher than that of low-dose group.
Conclusion

Xiaotan Sanjie Decoction-containing serum can inhibit the MKN-45 cell proliferation and induce cell apoptosis, which provides an experimental evidence for its treatment of human gastric cancer.

Key words: Compound (TCD), Gastric tumor, Serum pharmacology, Apoptosis, Proliferation

Table 1

Effects of Xiaotan Sanjie Decoction-containing serum on proliferation of MKN-45 cell line"

Group n OD (x±s) Proliferation rate (%)
Control
24 h 5 0.110 6±0.003 6 100
48 h 5 0.118 6±0.0036 100
72 h 5 0.162 6±0.004 5 100
96 h 5 0.221 0±0.004 3 100
Low-dose
24 h 5 0.109 0±0.004 2 98.55
48 h 5 0.116 8±0.003 7 98.48
72 h 5 0.156 0±0.001 6* 95.94
96 h 5 0.209 2±0.006 1* 94.66
Medium-dose
24 h 5 0.106 4±0.004 6 96.20
48 h 5 0.112 6±0.004 0* 94.94
72 h 5 0.122 0±0.002 9* 75.03
96 h 5 0.188 4±0.006 0* 85.25
High-dose
24 h 5 0.105 4±0.004 6 95.30
48 h 5 0.112 4±0.004 2* 94.77
72 h 5 0.125 2±0.003 3* 77.00
96 h 5 0.189 2±0.004 4* 85.61

Figure 1

Effects of Xiaotan Sanjie Decoction-containing serum on growth of MKN-45 cells"

Figure 2

Effects of Xiaotan Sanjie Decoction-containing serum on morphological changes of MKN-45 cells (Inverted microscopy, ×100)A: Normal cells; B: Control group; C: Low-dose group; D: Medium-dose group; E: High-dose group."

Figure 3

Effects of Xiaotan Sanjie Decoction-containing serum on apoptosis of MKN-45 cells"

[1] Update of Chinese version of NCCN gastric cancer clinical practice guidelines[J]. Henan Yi Xue Yan Jiu, 2008,17(2):186
NCCN胃癌临床实践指南(中国版)更新[J]. 河南医学研究, 2008,17(2):186
[2] Yang HP, Zhang CQ . Research status quo and progress in prognosis of gastric cancer[J]. Chongqing Yi Xue, 2008,37(8):879-881
杨海平, 张才全 . 影响胃癌预后因素的研究现状和进展[J]. 重庆医学, 2008,37(8):879-881
[3] Lu DL, Wei PK . Xiaotan Sanjie Recipe in treatment of advanced gastric cancer: a report of 15 cases[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2007,15(6):401-402
吕东来, 魏品康 . 消痰散结方治疗中晚期胃癌15例[J]. 中国中西医结合消化杂志, 2007,15(6):401-402
[4] Su XM, Wei PK, Xu L, Chen YL . Effects of Xiaotan Sanjie Recipe in inhibiting growth and metastasis of gastric carcinoma in nude mice[J]. Chengdu Zhong Yi Yao Da Xue Xue Bao, 2003,26(4):20-22
苏晓妹, 魏品康, 许玲, 陈亚琳 . 消痰散结方抑制胃癌生长和转移的实验研究[J]. 成都中医药大学学报, 2003,26(4):20-22
[5] Yang YF, Li JL . Apoptosis-related genes and human tumor[J]. Sheng Ming De Hua Xue, 2004,24(3):270-272
杨银峰, 李家林 . 细胞凋亡相关基因与人类肿瘤[J]. 生命的化学, 2004,24(3):270-272
[6] Li XY, Wei PK . Observation of curative effect on late gastric cancer treated by Jinlongshe oral liquid[J]. Hubei Zhong Yi Za Zhi, 2001,23(11):3-4
李相勇, 魏品康 . 金龙蛇口服液治疗晚期胃癌的疗效观察[J]. 湖北中医杂志, 2001,23(11):3-4
[7] Li GL, Gui SQ, Zhu DH, Chen SH, Ye Y . Effects of Pinellia extraction only or combined with cisplatin on growth of HeLa cells of cervical cancer[J]. Fu Dan Xue Bao Yi Xue Ban, 2007,34(6):869-872
李桂玲, 归绥琪, 朱德厚, 陈松华, 叶阳 . 掌叶半夏有效提取物单独或与顺铂联合对体外培养宫颈癌HeLa细胞的作用[J]. 复旦学报(医学版), 2007,34(6):869-872
[8] Liang L, Wei ZR . Research progress in Rhizoma Arisaematis[J]. Zhongguo Yi Yao Ji Shu Jing Ji Yu Guan Li, 2008,2(6):73-78
梁蕾, 魏征人 . 天南星的研究进展[J]. 中国医药技术经济与管理, 2008,2(6):73-78
[9] Yang ZH, Yin JY, Wei ZR, Yan WQ . Effects of Arisaema extracts on apoptosis of human hepatoma SMMC-7721 cells and the mechanism[J]. Zhongguo Lao Nian Xue Za Zhi, 2007,27(2):142-144
杨宗辉, 尹建元, 魏征人, 颜炜群 . 天南星提取物诱导人肝癌SMMC-7721细胞凋亡及其机制的实验研究[J]. 中国老年学杂志, 2007,27(2):142-144
[10] Massagué J . G1 cell-cycle control and cancer[J]. Nature, 2004,432(7015):298-306
doi: 10.1038/nature03094
[11] Ji YQ, Xie M, Zhang LX . Research progress of morphological changes of cell apoptosis and the related molecular regulation[J]. Wei Sheng Wu Xue Mian Yi Xue Jin Zhan, 2003,31(3):47-50
纪玉强, 谢明, 张亮轩 . 细胞凋亡的形态学变化过程及相关的分子调节研究进展[J]. 微生物学免疫学进展, 2003,31(3):47-50
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