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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 275-279.doi: 10.3736/jcim20100313

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huanglian Jiedu Decoction on blood lipid metabolism and its related gene expressions in rats with hyperlipidemia

 Jin Jina, Yang Zhanga, Wen-xiang Hub, Zheng-yao Zhanga, Nan-nan Xub, Qiu-li Zhoua   

  1. a Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun 130022, Jilin Province, China
    b Department of Chemistry, Capital Normal University, Beijing 100037, China
  • Received:2009-11-05 Accepted:2010-01-06 Online:2010-03-20 Published:2010-03-15
  • Contact: Qiu-li Zhou E-mail:qiulizhoujl@yahoo.com.cn

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Objective

To observe the effects of Huanglian Jiedu Decoction (HJD), a compound traditional Chinese herbal medicine, on lipid metabolism and its related gene expressions in rats with hyperlipidemia.
Methods

Fifty SD rats were randomly divided into normal control group, untreated group, Lipitor (atorvastatin) group, and low- and high-dose HJD groups. Except the normal control group, rats in the other groups were fed with high-fat diet to induce hyperlipidemia. Then the rats were administered with corresponding drugs for 8 weeks. After treatment, the serum levels of total cholesterol (TC), triacylglycerol (TAG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were assayed. The activities of lipoprotein lipase (LPL) and hepatic lipase (HL) in liver tissues were measured. Low-density lipoprotein receptor (LDLR) and peroxisome proliferator-activated receptor γ (PPARγ) mRNA expressions in liver tissues were determined by reverse transcription-polymerase chain reaction.
Results

Compared with the normal control group, the levels of serum TC, TAG and LDL-C in the untreated group were increased and the level of serum HDL-C was reduced. The activities of LPL and HL and expressions of LDLR and PPARγ mRNAs in the untreated group were lower than those in the normal control group. After treatment, high-dose HJD significantly improved hyperlipemia by decreasing TC, TAG and LDL-C and increasing HDL-C. The activities of LPL and HL and expression levels of LDLR and PPARγ mRNAs in liver tissues were also markedly enhanced in the high-dose HJD group as compared with those in the untreated group.
Conclusion

HJD can activate the activity of lipid metabolism enzyme, and enhance the expressions of LDLR and PPARγ mRNAs to modulate the lipid metabolic disorders in rats with hyperlipidemia.

Key words: Compound (TCD), Hyperlipidemia, Low-density lipoprotein receptor, Peroxisome proliferator- activated receptor y, Rats

Table 1

Levels of blood TC, TAG, HDL-C and LDL-C of rats in different groups ($\bar{x}±s$, mmol/L)"

Group n TC TAG LDL-C HDL-C
Normal control 8 1.68±0.48 1.27±0.66 0.51±0.18 1.08±0.18
Untreated 10 4.55±0.45** 3.71±0.15** 1.81±0.19** 0.57±0.13**
Lipitor 10 2.83±0.37△ 2.82±0.35△ 0.75±0.07△△ 0.85±0.14△△
Low-dose HJD 10 3.92±0.57 3.09±0.23 1.34±0.41 0.67±0.25△
High-dose HJD 10 3.14±0.48△ 2.62±0.54△ 0.71±0.26△△ 0.73±0.08△△

Table 2

Activities of LPL and HL in liver tissues of rats in different groups ($\bar{x}±s$, U/mg prot)"

Group n LPL HL
Normal control 8 8.23±0.35 4.25±0.38
Untreated 10 4.93±0.48** 2.67±0.32**
Lipitor 10 7.19±0.32△△ 7.23±0.24△△
Low-dose HJD 10 5.89±0.41 5.98±0.37△△
High-dose HJD 10 6.34±0.46△ 6.24±0.47△△

Figure 1

Expression levels of LDLR and PPARγ mRNAs in liver tissues of rats in different groups1: Normal control group; 2: Untreated group; 3: Lipitor group; 4: Low-dose HJD group; 5: High-dose HJD group; M: Marker."

Table 3

Expression levels of LDLR and PPARγ mRNAs in liver tissues of rats in different groups ($\bar{x}±s$)"

Group n LDLR/β-actin PPARγ/β-actin
Normal control 8 0.86±0.22 1.21±0.13
Untreated 10 0.61±0.14** 1.07±0.15**
Lipitor 10 0.79±0.18△ 1.47±0.20△△
Low-dose HJD 10 0.78±0.24△ 1.27±0.17△
High-dose HJD 10 0.90±0.12△△ 2.10±0.27△△
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