黄连解毒汤对高脂血症大鼠血脂代谢及其相关基因表达的影响

doi:10.3736/jcim20100313

Abstract

Objective

To observe the effects of Huanglian Jiedu Decoction (HJD), a compound traditional Chinese herbal medicine, on lipid metabolism and its related gene expressions in rats with hyperlipidemia.
Methods

Fifty SD rats were randomly divided into normal control group, untreated group, Lipitor (atorvastatin) group, and low- and high-dose HJD groups. Except the normal control group, rats in the other groups were fed with high-fat diet to induce hyperlipidemia. Then the rats were administered with corresponding drugs for 8 weeks. After treatment, the serum levels of total cholesterol (TC), triacylglycerol (TAG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were assayed. The activities of lipoprotein lipase (LPL) and hepatic lipase (HL) in liver tissues were measured. Low-density lipoprotein receptor (LDLR) and peroxisome proliferator-activated receptor γ (PPARγ) mRNA expressions in liver tissues were determined by reverse transcription-polymerase chain reaction.
Results

Compared with the normal control group, the levels of serum TC, TAG and LDL-C in the untreated group were increased and the level of serum HDL-C was reduced. The activities of LPL and HL and expressions of LDLR and PPARγ mRNAs in the untreated group were lower than those in the normal control group. After treatment, high-dose HJD significantly improved hyperlipemia by decreasing TC, TAG and LDL-C and increasing HDL-C. The activities of LPL and HL and expression levels of LDLR and PPARγ mRNAs in liver tissues were also markedly enhanced in the high-dose HJD group as compared with those in the untreated group.
Conclusion

HJD can activate the activity of lipid metabolism enzyme, and enhance the expressions of LDLR and PPARγ mRNAs to modulate the lipid metabolic disorders in rats with hyperlipidemia.

Key words: Compound (TCD), Hyperlipidemia, Low-density lipoprotein receptor, Peroxisome proliferator- activated receptor y, Rats

Jin Jin, Yang Zhang, Wen-xiang Hu, Zheng-yao Zhang, Nan-nan Xu, Qiu-li Zhou. Effects of Huanglian Jiedu Decoction on blood lipid metabolism and its related gene expressions in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2010, 8(3): 275-279.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20100313

http://www.jcimjournal.com/EN/Y2010/V8/I3/275

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References 10

[1]	Jin D, Lu FE, Chen G, Sun H, Lu XH . Effects of Huanglian Jiedu Decoction on phosphatidylinositol-3-kinase expression in target tissues of type 2 diabetic rats[J]. J Chin Integr Med, 2007,5(5):541-545
	金丹, 陆付耳, 陈广, 孙焕, 陆小红 . 黄连解毒汤对2型糖尿病大鼠靶组织磷脂酰肌醇3激酶的影响[J]. 中西医结合学报, 2007,5(5):541-545
[2]	Leng SH, Lu FE, Tu QN, Xu LJ, Yang MW, Wang KF . Effects of Huanglian Jiedu Decoction on blood glucose and lipids metabolisms in type 2 diabetic rats[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2003,9(4):4388-4389
	冷三华, 陆付耳, 屠庆年, 徐丽君, 杨明炜, 王开富 . 黄连解毒汤对2型糖尿病大鼠血糖和血脂代谢的影响[J]. 中国中医基础医学杂志, 2003,9(4):4388-4389
[3]	Ding LB, Lu FE, Ye AL, Xu LJ, Wang KF . Effects of Huanglian Jiedu Decoction on leptin and resistin in insulin resistive rats[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2006,26(3):232-235
	丁来标, 陆付耳, 叶爱丽, 徐丽君, 王开富 . 黄连解毒汤对胰岛素抵抗大鼠瘦素和抵抗素的影响[J]. 中国中西医结合杂志, 2006,26(3):232-235
[4]	Chira EC, McMillen TS, Wang S, Haw A 3rd, O’Brien KD, Wight TN, Chait A .Tesaglitazar, a dual peroxisome proliferator-activated receptor alpha/gamma agonist, reduces atherosclerosis in female low density lipoprotein receptor deficient mice[J]. Atherosclerosis, 2007,195(1):100-109 doi: 10.1016/j.atherosclerosis.2006.12.012
[5]	Chen KX . Comprehension on treatment based on typing of hyperlipidemia[J]. Shi Zhen Guo Yi Guo Yao, 2002,12(13):750-751
	陈康新 . 高脂血症的分型论治体会[J]. 时珍国医国药, 2002,12(13):750-751
[6]	Zhang Y, Xie ML, Zhu LJ, Gu ZL . Therapeutic effect of osthole on hyperlipidemic fatty liver in rats[J]. Acta Pharmacol Sin, 2007,28(3):398-403 doi: 10.1111/aphs.2007.28.issue-3
[7]	Stulnig TM, Klocker H, Harwood HJ Jr, Jürgens G, Schönitzer D, Jarosch E, Huber LA, Amberger A, Wick G .In vivo LDL receptor and HMG-CoA reductase regulation in human lymphocytes and its alterations during aging[J]. Arterioscler Thromb Vasc Biol, 1995,15(7):872-878 doi: 10.1161/01.ATV.15.7.872
[8]	Kong W, Wei J, Abidi P, Lin M, Inaba S, Li C, Wang Y, Wang Z, Si S, Pan H, Wang S, Wu J, Wang Y, Li Z, Liu J, Jiang JD . Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins[J]. Nat Med, 2004,10(12):1344-1351 doi: 10.1038/nm1135
[9]	Hammarstedt A, Andersson CX, Rotter Sopasakis V, Smith U . The effect of PPARgamma ligands on the adipose tissue in insulin resistance[J]. Prostaglandins Leukot Essent Fatty Acids, 2005,73(1):65-75 doi: 10.1016/j.plefa.2005.04.008
[10]	Huang C, Zhang Y, Gong Z, Sheng X, Li Z, Zhang W, Qin Y . Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway[J]. Biochem Biophys Res Commun, 2006,348(2):571-578 doi: 10.1016/j.bbrc.2006.07.095

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Group	n	TC	TAG	LDL-C	HDL-C
Normal control	8	1.68±0.48	1.27±0.66	0.51±0.18	1.08±0.18
Untreated	10	4.55±0.45**	3.71±0.15**	1.81±0.19**	0.57±0.13**
Lipitor	10	2.83±0.37^△	2.82±0.35^△	0.75±0.07^△△	0.85±0.14^△△
Low-dose HJD	10	3.92±0.57	3.09±0.23	1.34±0.41	0.67±0.25^△
High-dose HJD	10	3.14±0.48^△	2.62±0.54^△	0.71±0.26^△△	0.73±0.08^△△

Group	n	LPL	HL
Normal control	8	8.23±0.35	4.25±0.38
Untreated	10	4.93±0.48**	2.67±0.32**
Lipitor	10	7.19±0.32^△△	7.23±0.24^△△
Low-dose HJD	10	5.89±0.41	5.98±0.37^△△
High-dose HJD	10	6.34±0.46^△	6.24±0.47^△△

Group	n	LDLR/β-actin	PPARγ/β-actin
Normal control	8	0.86±0.22	1.21±0.13
Untreated	10	0.61±0.14**	1.07±0.15**
Lipitor	10	0.79±0.18^△	1.47±0.20^△△
Low-dose HJD	10	0.78±0.24^△	1.27±0.17^△
High-dose HJD	10	0.90±0.12^△△	2.10±0.27^△△

Effects of Huanglian Jiedu Decoction on blood lipid metabolism and its related gene expressions in rats with hyperlipidemia

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