[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (8): 767-773.doi: 10.3736/jcim20100807

• Original Experimental Research • Previous Articles     Next Articles

Effects of petroleum ether extract of Anacyclus pyrethrum DC. on sexual behavior in male rats

Sharma V,Thakur M,Chauhan NS,Dixit VK   

  1. Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya, Sagar 470003
  • Received:2010-04-07 Accepted:2010-04-26 Online:2010-08-20 Published:2010-08-15

Objective

The roots of Anacyclus pyrethrum DC. (Compositae) are employed in Ayurvedic system of medicine as “Vajikaran Rasayana” — a category of drugs for vitality and virility. They are believed to have aphrodisiac action. The present investigation was undertaken to evaluate their effects on sexual behavior in male rats.
Methods

Thirty-two male Wistar rats were divided into control group, testosterone group, low-dose (50 mg/kg) petroleum ether extract (PEE) group and high-dose (100 mg/kg) PEE group. PEE obtained from the roots of Anacyclus pyrethrum was administered orally to albino rats once daily, and 0.5 mg/kg (body weight) of testosterone was given intramuscularly twice weekly and served as positive control. The course of treatment was 28 days. The effects of PEE and testosterone on changes in body and accessory sexual organ weights, sexual behavior, penile erection and sexual performance were studied before treatment, after 15 and 28 days of treatment and 7 and 15 days after treatment.
Results

After 28 days of treatment, PEE and testosterone had a marked influence on body and accessory sexual organ weights as compared with arachis oil. The treated male rats were more receptive and oriented towards female rats and increased precopulatory activities like licking and sniffing of female anogenitals were observed. The penile erection index was significantly increased with reduction in mount latency and intromission latency period. There were four-fold increase in mount and three-fold increase in intromission frequency in treated rats reflecting improved sexual performance. The behavioral and sexual parameters were also observed after a lapse of 7 and 15 days of discontinuance of drug treatment.
Conclusion

Unlike testosterone, the PEE of Anacyclus pyrethrum shows efficacy in rats tested after the lapse of 7 and 15 days of discontinuation of treatment. This suggests that the drug has prolonged effect and capacitate the treated rats for improved sexual potential.

Key words: Anacyclus pyrethrum, Aphrodisiacs, Plant extracts, Petroleum ether extract, Sexual behavior, animal, Alkylamide, Rats

[1] Shamloul R . Natural aphrodisiacs[J]. J Sex Med, 2010,7(1 Pt 1):39-49
doi: 10.1111/j.1743-6109.2009.01521.x
[2] Sandroni P . Aphrodisiacs past and present: a historical review[J]. Clin Auton Res, 2001,11(5):303-307
doi: 10.1007/BF02332975 pmid: 11758796
[3] Acharya VYT .Charaka Samhita, with Ayurveda-Dipika commentary of Chakrapanidatta(5th ed) [M].Varanasi: Chaukhamba Sanskrit Samsthan, 2001,( 5th ed)
[4] Puri HS .Rasayana — Ayurvedic herbs for longevity and rejuvenation[M].London: Taylor & Francis, 2003
[5] Bentley R, Trimen H. Medicinal plants[M]. New Delhi: Asiatic Publishing House,2004:
[6] Mukerji B .The Indian pharmaceutical codex[M].New Delhi: Council of Scientific and Industrial Research, 1953
[7] Crombie L .Isolation and structure of an N-isobutyldienediynamide from pellitory(Anacyclus pyrethrum DC.) [J].Nature, 1954, 174: 832-833
doi: 10.1038/174832a0
[8] Bendjeddou D, Lalaoui K, Satta D . Immunostimulating activity of the hot water-soluble polysaccharide extracts of Anacyclus pyrethrum, Alpinia galanga and Citrullus colocynthis[J]. J Ethnopharmacol, 2003,88(2-3):155-160
doi: 10.1016/S0378-8741(03)00226-5
[9] Cicero AF, Bandieri E, Arletti R . Lepidium meyenii Walp. improves sexual behaviour in male rats independently from its action on spontaneous locomotor activity[J]. J Ethnopharmacol, 2001,75(2-3):225-229
doi: 10.1016/S0378-8741(01)00195-7
[10] Agmo A . Unconditioned sexual incentive motivation in the male Norway rat(Rattus norvegicus)[J]. J Comp Psychol, 2003,117(1):3-14
doi: 10.1037/0735-7036.117.1.3 pmid: 12735358
[11] Thakur M, Chauhan NS, Bhargava S, Dixit VK . A comparative study on aphrodisiac activity of some ayurvedic herbs in male albino rats[J]. Arch Sex Behav, 2009,38(6):1009-1015
doi: 10.1007/s10508-008-9444-8 pmid: 19139984
[12] Thakur M, Bhargava S, Praznik W, Loeppert R, Dixit VK . Effect of Chlorophytum Borivilianum Santapau and Fernandes on sexual dysfunction in hyperglycemic male rats[J]. Chin J Integr Med, 2009,15(6):448-453
doi: 10.1007/s11655-009-0448-6
[13] Islam MW, Tariq M, Ageel AM, al-Said MS, al-Yhya AM .Effect of Salvia haematodes on sexual behaviour of male rats[J]. J Ethnopharmacol, 1991,33(1-2):67-72
doi: 10.1016/0378-8741(91)90163-8 pmid: 1943176
[14] Benassi-Benelli A, Ferrari F, Quarantotti BP . Penile erection induced by apomorphine and N-n-propyl-norapomorphine in rats[J]. Arch Int Pharmacodyn Ther, 1979,242(2):241-247
pmid: 44457
[15] Chauhan NS, Rao ChV, Dixit VK .Effect of Curculigo orchioides rhizomes on sexual behaviour of male rats[J]. Fitoterapia, 2007,78(7-8):530-534
doi: 10.1016/j.fitote.2007.06.005 pmid: 17643866
[16] Thakur M, Dixit VK . Aphrodisiac activity of Dactylorhiza hatagirea(D. Don) Soo in male albino rats[J]. Evid Based Complement Alternat Med, 2007,4(Suppl 1):29-31
doi: 10.1093/ecam/nem111 pmid: 18227929
[17] Gulland JM, Hopton GU .Pellitorine, the pungent principle of Anacyclus pyrethrum[J].J Chem Soc, 1930: 6-11
doi: 10.1039/JR9300000006
[18] Chauhan NS, Sharma V, Dixit VK .Effect of Asteracantha longifolia seeds on the sexual behaviour of male rats[J].Nat Prod Res, 2009, 14: 1-9
doi: 10.1080/14786410802588493 pmid: 19753500
[19] Luo Q, Li Z, Huang X, Yan J, Zhang S, Cai YZ . Lycium barbarum polysaccharides: protective effects against heat-induced damage of rat testes and H2O2-induced DNA damage in mouse testicular cells and beneficial effect on sexual behavior and reproductive function of hemicastrated rats[J]. Life Sci, 2006,79(7):613-621
doi: 10.1016/j.lfs.2006.02.012
[20] Subramoniam A, Madhavachandran V, Rajasekharan S, Pushpangadan P . Aphrodisiac property of Trichopus zeylanicus extract in male mice[J]. J Ethnopharmacol, 1997,57(1):21-27
doi: 10.1016/S0378-8741(97)00040-8 pmid: 9234161
[21] Gonzales GF, Córdova A, Vega K, Chung A, Villena A, Góñez C .Effect of Lepidium meyenii(Maca), a root with aphrodisiac and fertility-enhancing properties, on serum reproductive hormone levels in adult healthy men[J]. J Endocrinol, 2003,176(1):163-168
doi: 10.1677/joe.0.1760163
[22] Zheng BL, He K, Kim CH, Rogers L, Shao Y, Huang ZY, Lu Y, Yan SJ, Qien LC, Zheng QY . Effect of a lipidic extract from Lepidium meyenii on sexual behavior in mice and rats[J]. Urology, 2000,55(4):598-602
doi: 10.1016/S0090-4295(99)00549-X pmid: 10736519
[23] Bivalacqua TJ, Liu T, Musicki B, Champion HC, Burnett AL . Endothelial nitric oxide synthase keeps erection regulatory function balance in the penis[J]. Eur Urol, 2007,51(6):1732-1740
doi: 10.1016/j.eururo.2006.10.061 pmid: 3328507
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[4] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[5] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[6] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[7] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[8] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[11] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[14] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[15] Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]