[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (2): 209-215.doi: 10.3736/jcim20110215

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Yinsiwei compound on spatial learning and memory ability and the ultrastructure of hippocampal neurons in a rat model of sporadic Alzheimer disease

 Yong-chang Diwu1,2(), Jin-zhou Tian1, Shi Jing1   

  1. 1. First Clinical Medical College,Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China
    2. Clinical Medical College of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China
  • Received:2010-10-17 Accepted:2010-11-08 Online:2011-02-20 Published:2011-02-15
  • Contact: Diwu Yong-chang E-mail:ycdw07@yahoo.com.cn

RichHTML

1

PDF (PC)

71

Like

Objective: To study the effects of Chinese herbal medicine Yinsiwei compound (YSW) on spatial learning and memory ability in rats with sporadic Alzheimer disease (SAD) and the ultrastructural basis of the hippocampal neurons.
Methods: A rat model of SAD was established by intracerebroventricular injection of streptozotocin. The rats were divided into six groups: sham-operation group, model group, donepezil control group, and YSW low, medium and high dose groups. Drug interventions were started on the 21st day after modeling and each treatment group was given the corresponding drugs by gavage for two months. Meanwhile, the model group and the sham-operation group were given the same volume of distilled water by gavage once a day for two months. The Morris water maze was adopted to test spatial learning and memory ability of the rats. The place navigation test and the spatial probe test were conducted. The escape latency, total swimming distance and swimming time in the target quadrant of the rats were recorded. Also, the hippocampus tissues of rats were taken out and the ultrastructure of hippocampus neurons were observed by an electron microscope.
Results: In the place navigation test, compared with the model group, the mean escape latency and the total swimming distance of the donepezil group and the YSW low, medium and high dose groups were significantly shortened (P<0.05 or P<0.01). In the space probe test, the swimming time of each treatment group in the target quadrant was significantly longer than that of the model group (P<0.05 or P<0.01). For most of the test period, the donepezil group had no significant change compared with the YSW low, medium and high dose groups, respectively. The ultrastructure of the hippocampus neurons under the electron microscope also confirmed the efficacy of the drug treatment.
Conclusion: Chinese herbal medicine YSW compound can improve spatial learning and memory impairment of rats with SAD. The ultrastructural basis may be that it can protect the microtubule structures of hippocampal neurons and prevent nerve axons from being damaged.

Key words: Alzheimer disease, drugs,Chinese herbal, maze learning, learning disorders, memory disorders, hippocampus, animal experimentation, rats

"

Figure 1

Comparison of average escape latency in the place navigation test of rats in each group YL: Yinsiwei compound of low dose; YM: Yinsiwei compound of medium dose; YH: Yinsiwei compound of high dose."

"

Figure 2

Comparison of swimming distance in the place navigation test of rats in each group YL: Yinsiwei compound of low dose; YM: Yinsiwei compound of medium dose; YH: Yinsiwei compound of high dose."

"

Figure 3

Microtubule structure of the hippocampal neuronal axons of rats in each group A: Sham-operation group (Electron microscopy, ×60 000); B: Model group (Electron microscopy, ×60 000); C: Donepezil group (Electron microscopy, ×50 000); D: Yinsiwei high dose group (Electron microscopy, ×50 000); E: Yinsiwei medium dose group (Electron microscopy, ×60 000); F: Yinsiwei low dose group (Electron microscopy, ×50 000)."

[1] Lobo A, Launer LJ, Fratiglioni L, Andersen K, Di Carlo A, Breteler MM, Copeland JR, Dartigues JF, Jagger C, Martinez-Lage J, Soininen H , Hofman A; the Neurologic Diseases in the Elderly Research Group.Prevalence of dementia and major subtypes in Europe: A collaborative study of population-based cohorts[J]. Neurology, 2000,54(11 Suppl 5):S4-S9
doi: 10.1212/WNL.54.1.4 pmid: 10636116
[2] Moceri VM, Kukull WA , Emanual I, van Belle G , Starr JR, Schellenberg GD, McCormick WC, Bowen JD, Teri L, Larson EB.Using census data and birth certificates to reconstruct the early-life socioeconomic environment and the relation to the development of Alzheimer’s disease[J]. Epidemiology, 2001,12(4):383-389
doi: 10.1097/00001648-200107000-00007
[3] Sharma M, Gupta YK . Intracerebroventricular injection of streptozotocin in rats produces both oxidative stress in the brain and cognitive impairment[J]. Life Sci, 2001,68(9):1021-1029
doi: 10.1016/S0024-3205(00)01005-5
[4] Tiwari V, Kuhad A, Bishnoi M, Chopra K . Chronic treatment with tocotrienol, an isoform of vitamin E, prevents intracerebroventricular streptozotocin-induced cognitive impairment and oxidative-nitrosative stress in rats[J]. Pharmacol Biochem Behav, 2009,93(2):183-189
doi: 10.1016/j.pbb.2009.05.009
[5] Hoyer S, Lannert H . Long-term effects of corticosterone on behavior, oxidative and energy metabolism of parietotemporal cerebral cortex and hippocampus of rats: comparison to intracerebroventricular streptozotocin[J]. J Neural Transm, 2008,115(9):1241-1249
doi: 10.1007/s00702-008-0079-7
[6] Ishrat T, Parveen K, Khan MM, Khuwaja G, Khan MB, Yousuf S, Ahmad A, Shrivastav P, Islam F . Selenium prevents cognitive decline and oxidative damage in rat model of streptozotocin-induced experimental dementia of Alzheimer’s type[J]. Brain Res, 2009,1281:117-127
doi: 10.1016/j.brainres.2009.04.010
[7] Paxinos G, Waston C. Translated by Zhuge QC.The rat brain in stereotaxic coordinates(3rd ed)[M]. Beijing: People’s Medical Publishing House, 2005
Paxinos G, Waston C. 诸葛启钏, 译.大鼠脑立体定向图谱(第3版)[M]. 北京: 人民卫生出版社, 2005
[8] Li AP, Zhao H, Li S, Piao H, Sun CK . Species differences of muridae in the learning and memory behavior in morris water maze[J]. Zhongguo Xing Wei Yi Xue Ke Xue, 2005,14(1):29-31
doi: 10.3760/cma.j.issn.1674-6554.2005.01.011
李爱萍, 赵慧, 李韶, 朴花, 孙长凯 . 不同鼠种在Morris水迷宫学习记忆行为中的种属差异[J]. 中国行为医学科学, 2005,14(1):29-31
doi: 10.3760/cma.j.issn.1674-6554.2005.01.011
[9] Riley KP, Snowdon DA, Markesbery WR . Alzheimer’s neurofibrillary pathology and the spectrum of cognitive function: findings from the Nun Study[J]. Ann Neurol, 2002,51(5):567-577
doi: 10.1002/(ISSN)1531-8249
[10] Gong CX, Liu F, Grundke-Iqbal I, Iqbal K . Post-translational modifications of tau protein in Alzheimer’s disease[J]. J Neural Transm, 2005,112(6):813-838
doi: 10.1007/s00702-004-0221-0
[11] Blokland A, Jolles J . Spatial learning deficit and reduced hippocampal ChAT activity in rats after an ICV injection of streptozotocin[J]. Pharmacol Biochem Behav, 1993,44(2):491-494
doi: 10.1016/0091-3057(93)90497-H
[12] de la Monte SM, Wands JR . Review of insulin and insulin-like growth factor expression, signaling, and malfunction in the central nervous system: relevance to Alzheimer’s disease[J]. J Alzheimers Dis, 2005,7(1):45-61
doi: 10.3233/JAD-2005-7106
[13] Sonkusare S, Srinivasan K, Kaul C, Ramarao P . Effect of donepezil and lercanidipine on memory impairment induced by intracerebroventricular streptozotocin in rats[J]. Life Sci, 2005,77(1):1-14
doi: 10.1016/j.lfs.2004.10.036
[14] Morris RG, Garrud P, Rawlins JN , O’Keefe J .Place navigation impaired in rats with hippocampal lesions[J]. Nature, 1982,297(5868):681-683
doi: 10.1038/297681a0 pmid: 7088155
[15] Zhang JT . Nootropic mechanisms of ginsenoside Rg1 — influence on neuronal plasticity and neurogenesis[J]. Yao Xue Xue Bao, 2005,40(5):385-388
doi: 10.3321/j.issn:0513-4870.2005.05.001
张均田 . 人参皂苷Rg1的促智作用机制——对神经可塑性和神经发生的影响[J]. 药学学报, 2005,40(5):385-388
doi: 10.3321/j.issn:0513-4870.2005.05.001
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[4] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[5] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[6] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[7] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[8] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[11] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[12] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[14] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[15] Ling Xu, Qiao-ling Zhou, Jie Han, Dan-ying Zhang, Hui-juan Dong, Zai-long Cai, Chao-qin Yu, Dong-xia Zhai . Effects of Chinese herbal medicine Neiyi Recipe-medicated serum on angiopoiesis of endometriosis in the chick chorioallantoic membrane model. Journal of Chinese Integrative Medicine, 2012, 10(7): 800-806.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]