大豆提取物抗戊四唑所致卵巢切除大鼠痉挛的作用

doi:10.3736/jcim20110606

Abstract

Objective: To investigate the effects of soy extract on pentylenetetrazol (PTZ)-induced seizures in ovariectomized (OVX) rats.
Methods: Female Wistar rats were randomly divided into 4 groups (n=15 in each group) as follows: sham-operated, OVX, low-dose soy (LDS) and high-dose soy (HDS). The rats in each group were divided into two subgroups and received daily injection of a low dose of PTZ (40 mg/kg body weight, intraperitoneally, n=7 in each subgroup) for 14 d or a single injection of a high dose of PTZ (90 mg/kg body weight, intraperitoneally, n=8 in each subgroup). The rats of LDS and HDS groups were injected with 20 and 60 mg/kg body weight of soy extract intraperitoneally, respectively, just 30 min before each PTZ injection. The rats of the sham-operated and the OVX groups received saline instead of soy extract. After treatment, the rats were placed in a plexiglas cage and their behaviors were observed for 60 min.
Results: The results of repeated injection of low dose of PTZ during 14 d showed that the seizure score of the rats of OVX group on days 3, 5, 8, 10, 11, 12, and 13 was lower than that of the sham-operated group (P<0.05 or P<0.01). However, the rats of both LDS and HDS groups had higher score compared with the OVX group on the mentioned days (P<0.05 or P<0.01). The results of a single injection of a high dose of PTZ showed a significant increase (P<0.01) in the generalized tonic-clonic seizure (GTCS), but not the minimal clonic seizure (MCS) in the OVX rats compared with the sham-operated rats. Treatment with both low and high doses of soy extract significantly decreased the GTCS and MCS latencies compared with the OVX group (P<0.01).
Conclusion: Female hormones affect seizure severity induced by PTZ, and phytoestrogens of soy mimic this effect. However, more investigations need to be done in the future.

Key words: soy, phytoestrogens, plant extracts, seizures, ovariectomy, pentylenetetrazol, rats

Ali Reza Ebrahimzadeh Bideskan, Mahmoud Hosseini, Toktam Mohammadpour, Reza Karami, Mehdi Khodamoradi, Habibollah Nemati Karimooy, Hassan Alavi. Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats. Journal of Chinese Integrative Medicine, 2011, 9(6): 611-618.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20110606

http://www.jcimjournal.com/EN/Y2011/V9/I6/611

[1]	Wyllie E . The treatment of epilepsy: principles and practice[M]. Philadelphia: Lippincott Williams and Wilkins, 2001,( 3rd ed):131-138
[2]	Fisher RS, van Emde Boas W, Blume W, Elger C, Genton P, Lee P, Engel J Jr . Epileptic seizures and epilepsy: definitions proposed by the International League Against Epilepsy(ILAE) and the International Bureau for Epilepsy(IBE)[J]. Epilepsia, 2005,46(4):470-472 doi: 10.1111/epi.2005.46.issue-4
[3]	Zahn CA, Morrell MJ, Collins SD, Labiner DM, Yerby MS . Management issues for women with epilepsy: a review of the literature[J]. Neurology, 1998,51(4):949-956 doi: 10.1212/WNL.51.4.949
[4]	Bauer J . Interactions between hormones and epilepsy in female patients[J]. Epilepsia, 2001,42(Suppl 3):20-22 doi: 10.1046/j.1528-1157.2001.042suppl.3020.x pmid: 11520317
[5]	Frye CA . Role of androgens in epilepsy[J]. Expert Rev Neurother, 2006,6(7):1061-1075 doi: 10.1586/14737175.6.7.1061 pmid: 16831119
[6]	Harden CL . Sexuality in men and women with epilepsy[J]. CNS Spectr, 2006,11(8Suppl 9):13-18 doi: 10.1017/S1092852900026717 pmid: 16871133
[7]	Herzog AG . Progesterone therapy in women with epilepsy: a 3-year follow-up[J]. Neurology, 1999,52(9):1917-1918 doi: 10.1212/WNL.52.9.1917-a pmid: 10371551
[8]	Hoffman GE, Moore N, Fiskum G, Murphy AZ . Ovarian steroid modulation of seizure severity and hippocampal cell death after kainic acid treatment[J]. Exp Neurol, 2003,182(1):124-134 doi: 10.1016/S0014-4886(03)00104-3
[9]	Velískov J . Estrogens and epilepsy: why are we so excited?[J]. Neuroscientist, 2007,13(1):77-88 doi: 10.1177/1073858406295827 pmid: 17229977
[10]	Edwards HE, Burnham WM, Mendonca A, Bowlby DA , MacLusky NJ.Steroid hormones affect limbic afterdischarge thresholds and kindling rates in adult female rats[J]. Brain Res, 1999,838(1-2):136-150 doi: 10.1016/S0006-8993(99)01619-4
[11]	Verrotti A, Latini G, Manco R, De Simone M, Chiarelli F . Influence of sex hormones on brain excitability and epilepsy[J]. J Endocrinol Invest, 2007,30(9):797-803 doi: 10.1007/BF03350821 pmid: 17993775
[12]	Yamano Y, Sakane S, Takamatsu J, Ohsawa N . Estrogen supplementation for bone dematuration in young epileptic man treated with anticonvulsant therapy: a case report[J]. Endocr J, 1999,46(2):301-307 doi: 10.1507/endocrj.46.301
[13]	Kotz K, Alexander JL, Dennerstein L . Estrogen and androgen hormone therapy and well-being in surgically postmenopausal women[J]. J Womens Health (Larchmt), 2006,15(8):898-908 doi: 10.1089/jwh.2006.15.898
[14]	Rapkin AJ, Winer SA . Drospirenone: a novel progestin[J]. Expert Opin Pharmacother, 2007,8(7):989-999 doi: 10.1517/14656566.8.7.989
[15]	Beral V ; Million Women Study Collaborators.Breast cancer and hormone-replacement therapy in the Million Women Study[J]. Lancet, 2003,362(9382):419-427 doi: 10.1016/S0140-6736(03)14065-2
[16]	Grady D, Gebretsadik T, Kerlikowske K, Ernster V, Petitti D . Hormone replacement therapy and endometrial cancer risk: a meta-analysis[J]. Obstet Gynecol, 1995,85(2):304-313 doi: 10.1016/0029-7844(94)00383-O pmid: 7824251
[17]	Hammond CB . Women’s concerns with hormone replacement therapy — compliance issues[J]. Fertil Steril, 1994,62(6 Suppl 2):157S-160S
[18]	Brown NM, Setchell KD . Animal models impacted by phytoestrogens in commercial chow: implications for pathways influenced by hormones[J]. Lab invest, 2001,81(5):735-747 doi: 10.1038/labinvest.3780282
[19]	Kurzer MS, Xu X . Dietary phytoestrogens[J]. Annu Rev Nutr, 1997,17:353-381 doi: 10.1146/annurev.nutr.17.1.353
[20]	Miksicek RJ . Estrogenic flavonoids: structural requirements for biological activity[J]. Proc Soc Exp Biol Med, 1995,208(1):44-50 doi: 10.3181/00379727-208-43830
[21]	Adlercreutz H, Mazur W . Phyto-oestrogens and Western diseases[J]. Ann Med, 1997,29(2):95-120 doi: 10.3109/07853899709113696 pmid: 9187225
[22]	File SE, Jarrett N, Fluck E, Duffy R, Casey K, Wiseman H . Eating soya improves human memory[J]. Psychopharmacology (Berl), 2001,157(4):430-436 doi: 10.1007/s002130100845 pmid: 11605103
[23]	Duffy R, Wiseman H, File SE . Improved cognitive function in postmenopausal women after 12 weeks of consumption of a soya extract containing isoflavones[J]. Pharmacol Biochem Behav, 2003,75(3):721-729 doi: 10.1016/S0091-3057(03)00116-3
[24]	Linford NJ, Dorsa DM . 17β-Estradiol and the phytoestrogen genistein attenuate neuronal apoptosis induced by the endoplasmic reticulum calcium-ATPase inhibitor thapsigargin[J]. Steroids, 2002,67(13-14):1029-1040 doi: 10.1016/S0039-128X(02)00062-4
[25]	Pan Y, Anthony M, Clarkson TB . Effect of estradiol and soy phytoestrogens on choline acetyltransferase and nerve growth factor mRNAs in the frontal cortex and hippocampus of female rats[J]. Proc Soc Exp Biol Med, 1999,221(2):118-125 doi: 10.3181/00379727-221-44393
[26]	Ferguson SA, Flynn KM, Delclos KB, Newbold RR, Gough BJ . Effects of lifelong dietary exposure to genistein or nonylphenol on amphetamine-stimulated striatal dopamine release in male and female rats[J]. Neurotoxicol Teratol, 2002,24(1):37-45 doi: 10.1016/S0892-0362(01)00193-3
[27]	Balduzzi R, Cupello A, Robello M . Modulation of the expression of GABAA receptors in rat cerebellar granule cells by protein tyrosine kinases and protein kinase C[J]. Biochim Biophys Acta, 2002,1564(1):263-270 doi: 10.1016/S0005-2736(02)00460-1
[28]	Zhu L, Jiang ZL, Krnjevi K, Wang FS, Ye JH . Genistein directly blocks glycine receptors of rat neurons freshly isolated from the ventral tegmental area[J]. Neuropharmacology, 2003,45(2):270-280 doi: 10.1016/S0028-3908(03)00151-5
[29]	Hosseini M, Sharifi MR, Alaei H, Shafei MN, Karimooy HA . Effects of angiotensin Ⅱ and captopril on rewarding properties of morphine[J]. Indian J Exp Biol, 2007,45(9):770-777 pmid: 17907742
[30]	Saffarzadeh F, Eslamizade MJ, Nemati Karimooy HA, Hadjzadeh MA, Khazaei M, Hosseini M . The effect of L-arginine on Morris water maze tasks of ovariectomized rats[J]. Acta Physiol Hung, 2010,97(2):216-223 doi: 10.1556/APhysiol.97.2010.2.8 pmid: 20511131
[31]	Racine RJ . Modification of seizure activity by electrical stimulation: cortical areas[J]. Electroencephalogr Clin Neurophysiol, 1975,38(1):1-12 doi: 10.1016/0013-4694(75)90204-7
[32]	Hosseini M, Sadeghnia HR, Salehabadi S, Alavi H, Gorji A . The effect of L-arginine and L-NAME on pentylenetetrazole induced seizures in ovariectomized rats, an in vivo study[J]. Seizure, 2009,18(10):695-698 doi: 10.1016/j.seizure.2009.09.008
[33]	Arai Y, Sekine Y, Murakami S . Estrogen and apoptosis in the developing sexually dimorphic preoptic area in female rats[J]. Neurosci Res, 1996,25(4):403-407 doi: 10.1016/0168-0102(96)01070-X pmid: 8866522
[34]	Miranda RC, Sohrabji F, Toran-Allerand D . Interactions of estrogen with the neurotrophins and their receptors during neural development[J]. Horm Behav, 1994,28(4):367-375 doi: 10.1006/hbeh.1994.1033 pmid: 7729805
[35]	Brinton RD . Impact of estrogen therapy on Alzheimer’s disease: a fork in the road?[J]. CNS drugs, 2004,18(7):405-422 doi: 10.2165/00023210-200418070-00001 pmid: 15139797
[36]	Dluzen DE, McDermott JL . Gender differences in neurotoxicity of the nigrostriatal dopaminergic system: implications for Parkinson’s disease[J]. J Gend Specif Med, 2000,3(6):36-42
[37]	Scharfman HE, MacLusky NJ .The influence of gonadal hormones on neuronal excitability, seizures, and epilepsy in the female[J]. Epilepsia, 2006,47(9):1423-1440 doi: 10.1111/j.1528-1167.2006.00672.x pmid: 16981857
[38]	Foldvary-Schaefer N, Falcone T . Catamenial epilepsy: pathophysiology, diagnosis, and management[J]. Neurology, 2003,61(6 Suppl 2):S2-S15 doi: 10.1212/WNL.61.6_suppl_2.S2 pmid: 14504304
[39]	Paoletti AM, Floris S, Mannias M, Orrù M, Crippa D, Orlandi R, Del Zompo M, Melis GB . Evidence that cyproterone acetate improves psychological symptoms and enhances the activity of the dopaminergic system in postmenopause[J]. J Clin Endocrinol Metab, 2001,86(2):608-612 doi: 10.1210/jcem.86.2.7179
[40]	Pisani G, Facioni L, Fiorani F, Pisani G . Psychosexual problems in menopause[J]. Minerva Ginecol, 1998,50(3):77-81 pmid: 9595919
[41]	Abbasi F, Krumholz A, Kittner SJ, Langenberg P . Effects of menopause on seizures in women with epilepsy. Epilepsia[J]. Epilepsia, 1999,40(2):205-210 doi: 10.1111/j.1528-1157.1999.tb02076.x pmid: 9952268
[42]	Gholipour T, Jabbarzadeh A, Riazi K, Rasouli A, Nezami BG, Sharifzadeh M, Dehpour AR . Role of nitric oxide in the anticonvulsive effect of progesterone[J]. Epilepsy Behav, 2008,13(4):579-584 doi: 10.1016/j.yebeh.2008.07.011 pmid: 18703162
[43]	Penovich PE, Helmers S . Catamenial epilepsy[J]. Int Rev Neurobiol, 2008,83:79-90 doi: 10.1016/S0074-7742(08)00004-4
[44]	Pereira M Jr, Soares JM Jr, Valente SG, Oliveira PB, Cavalheiro EA, Amado D, Baracat EC . Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats[J]. Maturitas, 2009,62(2):190-196 doi: 10.1016/j.maturitas.2008.10.014 pmid: 19111409
[45]	Velískov J . The role of estrogens in seizures and epilepsy: the bad guys or the good guys?[J]. Neuroscience, 2006,138(3):837-844 doi: 10.1016/j.neuroscience.2005.07.005 pmid: 16310960
[46]	Velískov J, Velísek L, Galanopoulou AS, Sperber EF . Neuroprotective effects of estrogens on hippocampal cells in adult female rats after status epilepticus[J]. Epilepsia, 2000,41(Suppl 6):S30-S35 doi: 10.1111/epi.2000.41.issue-s5
[47]	Galanopoulou AS, Alm EM, Velískov J . Estradiol reduces seizure-induced hippocampal injury in ovariectomized female but not in male rats[J]. Neurosci Lett, 2003,342(3):201-205 doi: 10.1016/S0304-3940(03)00282-9
[48]	Valente SG, Marques RH, Baracat EC, Cavalheiro EA, Naffah-Mazzacoratti MG, Amado D . Effect of hormonal replacement therapy in the hippocampus of ovariectomized epileptic female rats using the pilocarpine experimental model[J]. Epilepsy Res, 2008,82(1):46-56 doi: 10.1016/j.eplepsyres.2008.07.003
[49]	Scharfman HE, Goodman JH, Rigoulot MA, Berger RE, Walling SG, Mercurio TC, Stormes K, Maclusky NJ . Seizure susceptibility in intact and ovariectomized female rats treated with the convulsant pilocarpine[J]. Exp Neurol, 2005,196(1):73-86 doi: 10.1016/j.expneurol.2005.07.007
[50]	Czlonkowska AI, Krzascik P, Sienkiewicz-Jarosz H, Siemiatkowski M, Szyndler J, Bidziński A, Plaznik A . The effects of neurosteroids on picrotoxin-, bicuculline- and NMDA-induced seizures, and a hypnotic effect of ethanol[J]. Pharmacol Biochem Behav, 2000,67(2):345-353 doi: 10.1016/S0091-3057(00)00369-5
[51]	Kokate TG, Cohen AL, Karp E, Rogawski MA . Neuroactive steroids protect against pilocarpine- and kainic acid-induced limbic seizures and status epilepticus in mice[J]. Neuropharmacology, 1996,35(8):1049-1056 doi: 10.1016/S0028-3908(96)00021-4
[52]	Maguire JL, Stell BM, Rafizadeh M, Mody I . Ovarian cycle-linked changes in GABA_A receptors mediating tonic inhibition alter seizure susceptibility and anxiety[J]. Nat Neurosci, 2005,8(6):797-804 doi: 10.1038/nn1469
[53]	Riazi K, Honar H, Homayoun H, Rashidi N, Dehghani M, Sadeghipour H, Gaskari SA, Dehpour AR . Sex and estrus cycle differences in the modulatory effects of morphine on seizure susceptibility in mice[J]. Epilepsia, 2004,45(9):1035-1042 doi: 10.1111/epi.2004.45.issue-9
[54]	Murphy DD, Cole NB, Greenberger V, Segal M . Estradiol increases dendritic spine density by reducing GABA neurotransmission in hippocampal neurons[J]. J Neurosci, 1998,18(7):2550-2559 doi: 10.1523/JNEUROSCI.18-07-02550.1998
[55]	Wallis CJ, Luttge WG . Influence of estrogen and progesterone on glutamic acid decarboxylase activity in discrete regions of rat brain[J]. J Neurochem, 1980,34(3):609-613 doi: 10.1111/jnc.1980.34.issue-3
[56]	Scambia G, Mango D, Signorile PG, Anselmi Angeli RA, Palena C, Gallo D, Bombardelli E, Morazzoni P, Riva A, Mancuso S . Clinical effects of a standardized soy extract in postmenopausal women: a pilot study[J]. Menopause, 2000,7(2):105-111 doi: 10.1097/00042192-200007020-00006 pmid: 10746892
[57]	North American Menopause Society . Treatment of menopause-associated vasomotor symptoms: position statement of The North American Menopause Society[J]. Menopause, 2004,11(1):11-33 doi: 10.1097/01.GME.0000108177.85442.71
[58]	Kritz-Silverstein D, Von Mühlen D, Barrett-Connor E, Bressel MA . Isoflavones and cognitive function in older women: the SOy and Postmenopausal Health In Aging(SOPHIA) Study[J]. Menopause, 2003,10(3):196-202 doi: 10.1097/00042192-200310030-00004
[59]	Pan Y, Anthony M, Watson S, Clarkson TB . Soy phytoestrogens improve radial arm maze performance in ovariectomized retired breeder rats and do not attenuate benefits of 17β-estradiol treatment[J]. Menopause, 2000,7(4):230-235 doi: 10.1097/00042192-200007040-00004
[60]	Sarkaki A, Amani R, Badavi M, Moghaddam AZ, Aligholi H, Safahani M, Haghighizadeh MH . Pre-treatment effect of different doses of soy isoflavones on spatial learning and memory in an ovariectomized animal model of Alzheimer’s disease[J]. Pak J Biol Sci, 2008,11(8):1114-1119 doi: 10.3923/pjbs.2008.1114.1119
[61]	Sarkaki A, Badavi M, Aligholi H, Moghaddam AZ . Preventive effects of soy meal(+/–isoflavone) on spatial cognitive deficiency and body weight in an ovariectomized animal model of Parkinson’s disease[J]. Pak J Biol Sci, 2009,12(20):1338-1345 doi: 10.3923/pjbs.2009.1338.1345
[62]	Azcoitia I, Moreno A, Carrero P, Palacios S, Garcia-Segura LM . Neuroprotective effects of soy phytoestrogens in the rat brain[J]. Gynecol Endocrinol, 2006,22(2):63-69 doi: 10.1080/09513590500519161 pmid: 16603429
[63]	Schreihofer DA, Do KD, Schreihofer AM . High-soy diet decreases infarct size after permanent middle cerebral artery occlusion in female rats[J]. Am J Physiol Regul Integr Comp Physiol, 2005,289(1):R103-R108 doi: 10.1152/ajpregu.00642.2004
[64]	Liu YQ, Xin TR, Liang JJ, Wang WM, Zhang YY . Memory performance, brain excitatory amino acid and acetylcholinesterase activity of chronically aluminum exposed mice in response to soy isoflavones treatment[J]. Phytother Res, 2010,24(10):1451-1456 doi: 10.1002/ptr.v24:10
[65]	Garcia-Segura LM, Azcoitia I, DonCarlos LL, .Neuroprotection by estradiol[J]. Prog Neurobiol, 2001,63(1):29-60 doi: 10.1016/S0301-0082(00)00025-3
[66]	McEwen BS . Invited review: Estrogens effects on the brain: multiple sites and molecular mechanisms[J]. J Appl physiol, 2001,91(6):2785-2801 doi: 10.1080/10407780590945579 pmid: 11717247
[67]	Molteni A, Brizio-Molteni L, Persky V . In vitro hormonal effects of soybean isoflavones[J]. J Nutr, 1995,125(3 Suppl):751S-756S doi: 10.1002/1098-108X(199503)17:2<205::AID-EAT2260170216>3.0.CO;2-B pmid: 7884561
[68]	Sonee M, Sum T, Wang C, Mukherjee SK . The soy isoflavone, genistein, protects human cortical neuronal cells from oxidative stress[J]. Neurotoxicology, 2004,25(5):885-891 doi: 10.1016/j.neuro.2003.11.001 pmid: 15288519
[69]	Pan Y, Anthony M, Clarkson TB . Evidence for up-regulation of brain-derived neurotrophic factor mRNA by soy phytoestrogens in the frontal cortex of retired breeder female rats[J]. Neurosci Lett, 1999,261(1-2):17-20 doi: 10.1016/S0304-3940(98)00994-X
[70]	Zhao L, Chen Q, Diaz Brinton R . Neuroprotective and neurotrophic efficacy of phytoestrogens in cultured hippocampal neurons[J]. Exp Biol Med (Maywood), 2002,227(7):509-519 doi: 10.1046/j.1365-2362.2002.01043.x pmid: 12094016
[71]	Kuiper GG, Lemmen JG, Carlsson B, Corton JC, Safe SH, van der Saag PT, van der Burg B, Gustafsson JA, .Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β[J]. Endocrinology, 1998,139(10):4252-4263 doi: 10.1210/endo.139.10.6216 pmid: 9751507
[72]	Huang RQ, Dillon GH . Direct inhibition of glycine receptors by genistein, a tyrosine kinase inhibitor[J]. Neuropharmacology, 2000,39(11):2195-2204 doi: 10.1016/S0028-3908(00)00046-0 pmid: 10963763
[73]	Lovekamp-Swan T, Glendenning ML, Schreihofer DA .A high soy diet enhances neurotropin receptor and Bcl-Ⅹ_L gene expression in the brains of ovariectomized female rats[J].Brain Res, 2007, 1159: 54-66
[74]	Pan M, Li Z, Yeung V, Xu RJ . Dietary supplementation of soy germ phytoestrogens or estradiol improves spatial memory performance and increases gene expression of BDNF, TrkB receptor and synaptic factors in ovariectomized rats[J]. Nutr Metab (Lond), 2010,7:75 doi: 10.1186/1743-7075-7-75
[75]	Sohrabji F, Miranda RC, Toran-Allerand CD . Identification of a putative estrogen response element in the gene encoding brain-derived neurotrophic factor[J]. Proc Natl Acad Sci U S A, 1995,92(24):11110-11114 doi: 10.1073/pnas.92.24.11110
[76]	Singh M, Meyer EM, Simpkins JW . The effect of ovariectomy and estradiol replacement on brain-derived neurotrophic factor messenger ribonucleic acid expression in cortical and hippocampal brain regions of female Sprague-Dawley rats[J]. Endocrinology, 1995,136(5):2320-2324 doi: 10.1210/endo.136.5.7720680
[77]	Scharfman HE, Maclusky NJ . Similarities between actions of estrogen and BDNF in the hippocampus: coincidence or clue?[J]. Trends Neurosci, 2005,28(2):79-85 doi: 10.1016/j.tins.2004.12.005 pmid: 15667930

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Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats

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[8]	Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9]	Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10]	Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[11]	Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12]	C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13]	Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[14]	Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[15]	Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.