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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (6): 611-618.doi: 10.3736/jcim20110606

• Original Experimental Research • Previous Articles     Next Articles

Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats

Ali Reza Ebrahimzadeh Bideskan1, Mahmoud Hosseini2,3(), Toktam Mohammadpour3, Reza Karami2,3, Mehdi Khodamoradi1, Habibollah Nemati Karimooy2, Hassan Alavi4   

  1. 1. Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
    2. Neuroscience Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
    3. Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
    4. Neuroscience Research Center and Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Received:2011-01-18 Accepted:2011-02-13 Online:2011-06-20 Published:2011-06-15
  • Contact: Hosseini Mahmoud

Objective: To investigate the effects of soy extract on pentylenetetrazol (PTZ)-induced seizures in ovariectomized (OVX) rats.
Methods: Female Wistar rats were randomly divided into 4 groups (n=15 in each group) as follows: sham-operated, OVX, low-dose soy (LDS) and high-dose soy (HDS). The rats in each group were divided into two subgroups and received daily injection of a low dose of PTZ (40 mg/kg body weight, intraperitoneally, n=7 in each subgroup) for 14 d or a single injection of a high dose of PTZ (90 mg/kg body weight, intraperitoneally, n=8 in each subgroup). The rats of LDS and HDS groups were injected with 20 and 60 mg/kg body weight of soy extract intraperitoneally, respectively, just 30 min before each PTZ injection. The rats of the sham-operated and the OVX groups received saline instead of soy extract. After treatment, the rats were placed in a plexiglas cage and their behaviors were observed for 60 min.
Results: The results of repeated injection of low dose of PTZ during 14 d showed that the seizure score of the rats of OVX group on days 3, 5, 8, 10, 11, 12, and 13 was lower than that of the sham-operated group (P<0.05 or P<0.01). However, the rats of both LDS and HDS groups had higher score compared with the OVX group on the mentioned days (P<0.05 or P<0.01). The results of a single injection of a high dose of PTZ showed a significant increase (P<0.01) in the generalized tonic-clonic seizure (GTCS), but not the minimal clonic seizure (MCS) in the OVX rats compared with the sham-operated rats. Treatment with both low and high doses of soy extract significantly decreased the GTCS and MCS latencies compared with the OVX group (P<0.01).
Conclusion: Female hormones affect seizure severity induced by PTZ, and phytoestrogens of soy mimic this effect. However, more investigations need to be done in the future.

Key words: soy, phytoestrogens, plant extracts, seizures, ovariectomy, pentylenetetrazol, rats

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