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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (6): 638-642.doi: 10.3736/jcim20110610

• Original Experimental Research • Previous Articles     Next Articles

Effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS

Bao Tan1,2, Hai-lian Shi1,2,3,4, Guang Ji1,2, Jian-qun Xie1,2()   

  1. 1．Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    3. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    4. Shanghai Key Laboratory of Complex Prescription, Shanghai 201203, China
  • Received:2011-01-10 Accepted:2011-01-25 Online:2011-06-20 Published:2011-06-15
  • Contact: Xie Jian-qun E-mail:xiejq01@gmail.com

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Objective: To investigate the effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS cells.
Methods: The inhibitory effects of taraxerol and taraxeryl acetate at different concentrations on AGS cell growth were measured by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and the concentrations of taraxerol and taraxeryl acetate to be used in following experiments were decided. Then, cell cycle analysis was performed by FACScan flow cytometry after culture with taraxerol or taraxeryl acetate. Annexin V-fluorescein isothiocyanate/propidium iodide staining was used to measure cell apoptosis.
Results: Taraxerol significantly inhibited AGS cell proliferation in a dose- and time-dependent manner. Taraxerol arrested the AGS cells at G2/M stage. 110 μmol/L taraxerol elevated the population of AGS cells arrested in G2/M phase compared with solvent (P<0.05). Taraxerol also promoted early cell apoptosis in AGS cells. 110 μmol/L taraxerol increased the early cell apoptosis rate from 4.45% to 10.29%, which was 1.31 times higher than that of the untreated cells. However, taraxeryl acetate had a lower inhibitory effect than taraxerol, and it showed a tendency of G2/M arrest and apoptosis promotion but with no statistical significance (P>0.05).
Conclusion: Taraxerol has inhibitory effects on AGS cell growth through inducing G2/M arrest and promotion of cell apoptosis. Taraxeryl acetate has less effect on cell cycle arrest and apoptosis of AGS cells than taraxerol.

Key words: Taraxacum mongolicum , drugs,Chinese herbal, gastric cancer, apoptosis, cell cycle

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Figure 1

Effects of taraxerol and taraxeryl acetate on cell apoptosis of AGS cells UL: left upper quadrant; UR: right upper quadrant; LL: left lower quadrant; LR: right lower quadrant."

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