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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (7): 746-751.doi: 10.3736/jcim20110708

• Original Experimental Research • Previous Articles     Next Articles

Effects of Indian herbal formulation Body Revival on human platelet aggregation and myocardial ischemia in rats

Tapas Kumar Sur, Biswajit Auddy, Dipankar Bhattacharyya()   

  1. Department of Pharmacology, Institute of Postgraduate Medical Education and Research, West Bengal University of Health Sciences,Kolkata 700020, West Bengal, India
    Department of Pharmacology, Institute of Postgraduate Medical Education and Research, West Bengal University of Health Sciences, Kolkata 700020, West Bengal, India
  • Received:2011-02-15 Accepted:2011-04-06 Online:2011-07-20 Published:2011-06-15
  • Supported by:
    M/s Health Reactive (Baddi, Solan, Himachal Pradesh, India) provid the research grant.

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Objective: To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human platelet aggregation and isoproterenol (IS)-induced myocardial ischemia (MI) damage in male Wistar rats.
Methods: BR suspension 10, 20 and 30 μg was mixed with platelet-rich plasma and incubated at 37 ℃ for 30 min, respectively. Then, adenosine diphosphate (ADP, 20 mmol/L) or collagen (2 μg) was added in the mixture and the aggregation was observed against platelet-poor plasma mixed with equal volume of suspension of the same test samples. Wistar rats divided into 4 groups were used to investigate BR’s effects on IS-induced MI. Levels of serum creatinine kinase (CK), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated by standard commercial biological kits. Serum nitric oxide (NOx) was also measured. The lipid peroxides (LPO) and protein concentrations in heart tissues were measured.
Results: BR could inhibit ADP- or collagen-induced human platelet aggregation dose-dependently. Moreover, it could protect MI caused by IS in rats. BR reduced the levels of serum CK, AST, ALT and NOx dose-dependently and also lowered LPO in heart tissues in comparison with the MI control (P<0.01).
Conclusion: BR can inhibit human platelet aggregation and protect MI caused by IS in rats.

Key words: platelet aggregation inhibitors, myocardial ischemia, lipid peroxidation, nitric oxide, plant extracts, rats

"

Group n ADP-induced platelet
aggregation (%)
ADP control (ADP 20 mmol/L) 6 86.23±0.51
BR 10 μg 6 65.66±0.23**
BR 20 μg 6 51.41±0.45**
BR 30 μg 6 46.53±0.48**

"

Group n Collagen-induced platelet
aggregation (%)
Collagen control (collagen 2 μg) 6 72.10±0.49
BR 10 μg 6 59.68±0.38△△
BR 20 μg 6 48.37±0.31△△
BR 30 μg 6 44.51±0.28△△

"

Group n Serum level Heart tissue
LPO (nmol/L)
CK (U/L) AST (U/L) ALT (U/L) NOx (μmol/L)
Normal control 6 88.10±3.69 38.01±1.23 46.50±1.28 13.83±1.22 0.71±0.02
Ischemic model control (IS 85 mg/kg) 6 268.50±8.28▲▲ 90.65±2.57▲▲ 106.62±2.38▲▲ 40.83±1.01▲▲ 2.16±0.09▲▲
IS (85 mg/kg)+BR (200 mg/kg) 6 193.50±4.42□□ 62.16±1.47□□ 82.15±3.02□□ 28.82±0.60□□ 1.54±0.03□□
IS (85 mg/kg)+BR (400 mg/kg) 6 159.65±5.76□□ 50.51±1.25□□ 69.84±1.70□□ 24.31±0.88□□ 1.29±0.06□□
[1] Reddy KS . India wakes up to the threat of cardiovascular diseases[J]. J Am Coll Cardiol, 2007,50(14):1370-1372
doi: 10.1016/j.jacc.2007.04.097 pmid: 17903637
[2] Yusuf S, Hawken S, Ounpuu S, Dans T, Avezum A, Lanas F, McQueen M, Budaj A, Pais P, Varigos J, Lisheng L ; INTERHEART Study Investigators.Effect of potentially modifiable risk factors associated with myocardial infarction in 52 countries(the INTERHEART study): case-control study[J]. Lancet, 2004,364(9438):937-952
doi: 10.1016/S0140-6736(04)17018-9
[3] Teoh M, Lalondrelle S, Roughton M, Grocott-Mason R, Dubrey SW . Acute coronary syndromes and their presentation in Asian and Caucasian patients in Britain[J]. Heart, 2007,93(2):183-188
doi: 10.1136/hrt.2006.091900 pmid: 16914486
[4] Cua TK, Koh HL . Medicinal plants as potential sources of lead compounds with anti-platelet and anti-coagulant activities[J]. Mini Rev Med Chem, 2006,6(6):611-624
doi: 10.2174/138955706777435751
[5] Kaul S, Kapoor NK . Reversal of changes of lipid peroxide, xanthine oxidase and superoxide dismutase by cardio-protective drugs in isoproterenol induced myocardial necrosis in rats[J]. Indian J Exp Biol, 1989,27(7):625-627
[6] Mishra LC. Scientific basis of Ayurvedic therapies[M]. New York: CRC Press, 2004: 511-534
[7] Rajadurai M, Prince PS . Comparative effects of Aegle marmelos extract and alpha-tocopherol on serum lipids, lipid peroxides and cardiac enzyme levels in rats with isoproterenol-induced myocardial infarction[J]. Singapore Med J, 2005,46(2):78-81
[8] Parab RS, Mengi SA . Hypolipidemic activity of Acorus calamus L. in rats[J]. Fitoterapia, 2002,73(6):451-455
doi: 10.1016/S0367-326X(02)00174-0 pmid: 12385866
[9] Dwivedi S, Somani PN, Chansouria JP, Udupa KN . Cardioprotective effects of certain indigenous drugs in myocardial ischaemia in rabbits[J]. Indian J Exp Biol, 1988,26(12):969-975
pmid: 3254869
[10] Bheemasankara Rao C, Namosiva Rao T, Muralikrishna B . Flavonoids from Blumea lacera[J]. Planta Med, 1977,31(3):235-237
doi: 10.1055/s-0028-1097520 pmid: 866481
[11] Punna R, Rao Paruchuri U . Effect of maturity and processing on total, insoluble and soluble dietary fiber contents of Indian green leafy vegetables[J]. Int J Food Sci Nutr, 2004,55(7):561-567
doi: 10.1080/09637480500126418
[12] Chang LC, Chvez D, Gills JJ, Fong HHS, Pezzuto JM, Kinghorn AD. .Rubiasins A — C, new anthracene derivatives from the roots and stems of Rubia cordifolia[J]. Tetrahedron Lett, 2000,41(37):7157-7162
doi: 10.1016/S0040-4039(00)01205-3
[13] Bombale SL, Gowda PHR, Malatheshaiah NT, Bombale DL . In vitro regeneration and transformation of Muskmelon(Cucumis melo L.) with alternative AtWBC19 marker gene[J]. Int J Biotechnol Biochem, 2010,6(1):1-12
[14] Ahmad VU, Rashid MA, Abbasi MA, Rasool N, Zubair M . New salirepin derivatives from Symplocos racemosa[J]. J Asian Nat Prod Res, 2007,9(3-5):209-215
doi: 10.1080/10286020500531993 pmid: 17566912
[15] Ali AT, al-Swayeh OA, al-Humayyd MS, Mustafa AA, al-Rashed RS, al-Tuwaijiri AS . Natural honey prevents ischaemia-reperfusion-induced gastric mucosal lesions and increased vascular permeability in rats[J]. Eur J Gastroenterol Hepatol, 1997,9(11):1101-1107
doi: 10.1097/00042737-199711000-00014
[16] Guide for the care and use of laboratory animals (NIH publication No.85-23). Revised 1985.
[17] Born GV . Adenosine diphosphate as a mediator of platelet aggregation in vivo: an editorial view[J]. Circulation, 1985,72(4):741-746
doi: 10.1007/978-1-4615-9442-0_28 pmid: 3914836
[18] Sur TK, Biswas TK, Ali L, Mukherjee B . Anti-inflammatory and anti-platelet aggregation activity of human placental extract[J]. Acta Pharmacol Sin, 2003,24(2):187-192
doi: 10.1016/S0731-7085(02)00572-1 pmid: 12546729
[19] Knufman NM, van der Laarse A, Vliegen HW, Brinkman CJ . Quantification of myocardial necrosis and cardiac hypertrophy in isoproterenol-treated rats[J]. Res Commun Chem Pathol Pharmacol, 1987,57(1):15-32
doi: 10.1016/0262-1746(87)90166-1 pmid: 2959998
[20] Bhargava AS, Preus M, Khater AR, Günzel P . Effect of iloprost on serum creatine kinase and lactate dehydrogenase isoenzymes after isoprenaline-induced cardiac damage in rats[J]. Arzneimittelforschung, 1990,40(3):248-252
[21] Bhattacharyya D, Pandit S, Jana U, Sen S, Sur TK . Hepatoprotective activity of Adhatoda vasica aqueous leaf extract on D-galactosamine-induced liver damage in rats[J]. Fitoterapia, 2005,76(2):223-225
doi: 10.1016/j.fitote.2004.10.014
[22] Lyle N, Bhattacharyya D, Sur TK, Munshi S, Paul S, Chatterjee S, Gomes A . Stress modulating antioxidant effect of Nardostachys jatamansi[J]. Indian J Biochem Biophys, 2009,46(1):93-98
doi: 10.1007/s00249-008-0372-2 pmid: 19374260
[23] Buege JA, Aust SD . Microsomal lipid peroxidation[J]. Methods Enzymol, 1978,52:302-310
doi: 10.1016/S0076-6879(78)52032-6
[24] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ . Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951,193(1):265-275
[25] Kumari SS, Menon VP . Changes in levels of lipid peroxides and activities of superoxide dismutase and catalase in isoproterenol induced myocardial infarction in rats[J]. Indian J Exp Biol, 1987,25(6):419-423
[26] Bhandari U, Ansari MN, Islam F . Cardioprotective effect of aqueous extract of Embelia ribes Burm fruits against isoproterenol-induced myocardial infarction in albino rats[J]. Indian J Exp Biol, 2008,46(1):35-40
[27] Wildenthal K, Decker RS, Poole AR, Griffin EE, Dingle JT . Sequential lysosomal alterations during cardiac ischemia. Ⅰ. Biochemical and immunohistochemical changes[J]. Lab Invest, 1978,38(6):656-661
[28] Sasikumar CS, Devi CS . Effect of Abana an Ayurvedic formulation, on lipid peroxidation in experimental myocardial infarction in rats[J]. Indian J Exp Biol, 2000,38(8):827-830
[29] Manson JE, Gaziano JM, Jonas MA, Hennekens CH . Antioxidants and cardiovascular disease: a review[J]. J Am Coll Nutr, 1993,12(4):426-432
doi: 10.1080/07315724.1993.10718332 pmid: 8409105
[30] Fu Y, Wang Z, Chen WL, Moore PK, Zhu YZ . Cardioprotective effects of nitric oxide-aspirin in myocardial ischemia-reperfused rats[J]. Am J Physiol Heart Circ Physiol, 2007,293(3):H1545-H1552
doi: 10.1152/ajpheart.00064.2007
[31] Ignarro LJ, Buga GM, Wood KS, Byrns RE, Chaudhuri G . Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc Natl Acad Sci US A. 1987; 84(24):9265-9269.
[32] Lakshmi SV, Padmaja G, Kuppusamy P, Kutala VK . Oxidative stress in cardiovascular disease[J]. Indian J Biochem Biophys, 2009,46(6):421-440
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