[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (9): 1049-1055.doi: 10.3736/jcim20120914

Previous Articles     Next Articles

Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats

Lawrence A. Olatunji1(),Taofeek O. Usman1, Joseph O. Adebayo2, Victoria A. Olatunji3   

  1. 1. Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin 240001, Nigeria
    2. Department of Biochemistry, Faculty of Science, University of Ilorin, Ilorin 240001, Nigeria
    3. Department of Ophthalmology, University of Ilorin Teaching Hospital, Ilorin 240001, Nigeria
  • Received:2012-02-17 Accepted:2012-03-31 Online:2012-09-20 Published:2018-09-15
  • Contact: A. Olatunji Lawrence E-mail:tunjilaw@unilorin.edu.ng

RichHTML

3

PDF (PC)

145

Like

Objective: To investigate the effects of oral administration of aqueous extract of Hibiscus sabdariffa on renal Na +-K +-ATPase and Ca 2+-Mg 2+-ATPase activities in rats.

Methods: The 25 and 50 mg/(kg·d) of aqueous extracts of H. sabdariffa were respectively given to rats in the experimental groups for 28 d, and rats in the control group received an appropriate volume of distilled water as vehicle. Na +-K +-ATPase and Ca 2+-Mg 2+-ATPase activities in the kidney were assayed by spectrophotometric method.

Results: Administrations of 25 and 50 mg/(kg·d) of aqueous extract of H. sabdariffa significantly decreased the Ca 2+-Mg 2+-ATPase activity in the kidney of rats (P<0.05). However, the renal Na +-K +-ATPase activity of the experimental rats was not affected by either dose of the extract. And the plasma Na +, K + and Ca 2+ levels of the experimental rats had no significant changes. Administration of either dose of the extract did not result in any significant changes in body and kidney weights, the concentrations of plasma albumin and total protein, and alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase activities. However, concentrations of creatinine and urea were significantly reduced by 50 mg/kg of the extract (P<0.05).

Conclusion: The present study indicates that oral administration of aqueous extract of H. sabdariffa may preserve the renal function despite a decreased renal Ca 2+-Mg 2+-ATPase activity. KEYWORD: Hibiscus; Ca 2+-Mg 2+-ATPase; sodium-potassium-exchanging ATPase; kidney function tests; rats

Key words: Hibiscus, Ca2+-Mg2+-ATPase, sodium-potassium-exchanging ATPase, kidney function tests, rats

"

Group n Body weight (g) Ratio of kidney weight to body weight
Control 6 178.2±12.2 0.61±0.05
Extract of Hibiscus sabdariffa (25 mg/kg) 6 181.3±9.7 0.64±0.02
Extract of Hibiscus sabdariffa (50 mg/kg) 6 180.8±11.4 0.65±0.04

"

Group n ALP AST ALT
Control 6 208.5±15.5 87.5±10.1 10.4±1.4
Extract of Hibiscus sabdariffa (25 mg/kg) 6 212.6±13.1 101.3±13.0 13.1±4.1
Extract of Hibiscus sabdariffa (50 mg/kg) 6 221.4±20.2 95.2±11.4 12.2±2.4

Figure 1

Effects of aqueous extract of Hibiscus sabdariffa on Ca2+ -Mg2+-ATPase activity in rat kidneyData are presented as mean ± standard error of mean, n=6; *P<0.05, vs control group. "

Figure 2

Effects of aqueous extract of Hibiscus sabdariffa on Na+ -K+-ATPase activity in rat kidneyData are presented as mean ± standard error of mean, n=6. "

"

Group n Sodium Potassium Calcium
Control 6 142.2±1.2 5.0±0.1 2.4±0.1
Extract of Hibiscus sabdariffa (25 mg/kg) 6
 140.0±1.1
 5.3±0.2
 2.1±0.2
Extract of Hibiscus sabdariffa (50 mg/kg) 6
 143.4±1.1
 5.2±0.2
 2.2±0.1

"

Group n Plasma total protein (g/L) Albumin (g/L) Creatinine (mg/L) Urea (mg/L)
Control 6 6.48±0.06 3.65±0.07 6.05±0.20 0.47±0.01
Extract of Hibiscus sabdariffa (25 mg/kg) 6 6.78±0.11 3.83±0.12 5.95±0.38 0.46±0.03
Extract of Hibiscus sabdariffa (50 mg/kg) 6 6.62±0.06 3.64±0.06 4.89±0.12* 0.37±0.03*
[1] Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D . Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study[J]. Lancet, 1993,342(8878):1007-1011
doi: 10.1016/0140-6736(93)92876-U
[2] Hertog MG, Kromhout D, Aravanis C, Blackburn H, Buzina R, Fidanza F, Giampaoli S, Jansen A, Menotti A, Nedeljkovic S, Pekkarinen M, Simic BS, Toshima H, Feskens EJM, Hollman PCH, Katan MB . Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study[J]. Arch Intern Med, 1995,155(4):381-386
doi: 10.1001/archinte.1995.00430040053006
[3] Kromhout D, Bloemberg BP, Feskens EJ, Hertog MG, Menotti A, Blackburn H . Alcohol, fish, fibre and antioxidant vitamins intake do not explain population differences in coronary heart disease mortality[J]. Int J Epidemiol, 1996,25(4):753-759
doi: 10.1093/ije/25.4.753
[4] Adegunloye BJ, Omoniyi JO, Owolabi OA, Ajagbonna OP, Sofola OA, Coker HA . Mechanisms of the blood pressure lowering effect of the calyx extract of Hibiscus sabdariffa in rats. Afr[J] Med Med Sci. 1996; 25(3):235-238.
[5] Haji Faraji M, Haji Tarkhani A . The effect of sour tea (Hibiscus sabdariffa) on essential hypertension.J Ethnopharmacol. 1999; 65(3):231-236.
doi: 10.1016/S0378-8741(98)00157-3
[6] Onyenekwe PC, Ajani EO, Ameh DA, Gamaniel KS . Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in Wistar rats. Cell Biochem Funct. 1999; 17(3):199-206.
doi: 10.1002/(ISSN)1099-0844
[7] Ali MB, Salih WM, Mohamed AH, Homeida AM . Investigation of the antispasmodic potential of Hibiscus sabdariffa calyces.[J] Ethnopharmacol. 1991; 31(2):249-257.
doi: 10.1016/0378-8741(91)90009-3
[8] Odigie IP, Ettarh RR, Adigun SA . Chronic administration of aqueous extract of Hibiscus sabdariffa attenuates hypertension and reverses cardiac hypertrophy in 2K-1C hypertensive rats.[J] Ethnopharmacol. 2003; 86(2-3):181-185.
doi: 10.1016/S0378-8741(03)00078-3
[9] Ojeda D, Jiménez-Ferrer E, Zamilpa A, Herrera-Arellano A, Tortoriello J, Alvarez L . Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa. J Ethnopharmacol. 2010; 127(1):7-10.
doi: 10.1016/j.jep.2009.09.059
[10] Hibiscus sabdariffa L. calyces[J]. J Ethnopharmacol, 2007,109(3):388-393
doi: 10.1016/j.jep.2006.08.005
[11] Herrera-Arellano A, Flores-Romero S , Chávez-Soto MA, Tortoriello J. Effectiveness and tolerability of a standardized extract from Hibiscus sabdariffa in patients with mild to moderate hypertension: a controlled and randomized clinical trial. Phytomedicine. 2004; 11(5):375-382.
doi: 10.1016/j.phymed.2004.04.001
[12] Hibiscus sabdariffa on patients with hypertension. A randomized, double-blind, lisinopril-controlled clinical trial[J]. Planta Med, 2007,73(1):6-12
[13] Jonadet M, Bastide J, Bastide P, Boyer B, Carnat AP, Lamaison JL. In vitro enzyme inhibitory and in vivo cardioprotective activities of hibiscus ( Hibiscus sabdariffa L.). J Pharm Belg, 1990,45(2):120-124French with abstract in English
[14] Mozaffari-Khosravi H, Jalali-Khanabadi BA, Afkhami-Ardekani M, Fatehi F, Noori-Shadkam M . The effects of sour tea (Hibiscus sabdariffa) on hypertension in patients with type Ⅱ diabetes. J Hum Hypertens. 2009; 23(1):48-54.
doi: 10.1038/jhh.2008.100
[15] Wang SC, Lee SF, Wang CJ, Lee CH, Lee WC, Lee HJ . Aqueous extract from Hibiscus sabdariffa Linnaeus ameliorate diabetic nephropathy via regulating oxidative status and Akt/Bad/14-3-3γ in an experimental animal model. Evid Based Complement Alternat Med. 2011; 2011:938126.
[16] Hibiscus sabdariffa L . against oxidative stress in rat primary hepatocytes[J]. Food Chem Toxicol, 1997,35(12):1159-1164
doi: 10.1016/S0278-6915(97)85468-3
[17] Salleh MN, Runnie I, Roach PD, Mohamed S, Abeywardena MY . Inhibition of low-density lipoprotein oxidation and up-regulation of low-density lipoprotein receptor in HepG2 cells by tropical plant extracts[J]. J Agric Food Chem, 2002,50(13):3693-3697
doi: 10.1021/jf011593f
[18] Tee P, Yusof S, Mohamed S .) Antioxidative properties of roselle {Hibiscus sabdariffa L. ) in linoleic acid model system[J]. Nutr Food Sci, 2002,32(1):17-20
doi: 10.1108/00346650210413951
[19] Tsai PJ,McIntosh J,Pearce P,Camden B,Jordan BR. .) Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L. ) extract.[J]. Food Res Int, 2002,35(4):351-356
doi: 10.1016/S0963-9969(01)00129-6
[20] el-Saadany SS, Sitohy MZ, Labib SM, el-Massry RA . Biochemical dynamics and hypocholesterolemic action of Hibiscus sabdariffa (Karkade). Nahrung. 1991; 35(6):567-576.
[21] el-Saadany SS, Sitohy MZ, Labib SM, el-Massry RA . Biochemical dynamics and hypocholesterolemic action of Hibiscus sabdariffa (Karkade)[J]. Nutr Food Sci, 2002,32(5):190-196
doi: 10.1108/00346650210445758
[22] Chen CC, Hsu JD, Wang SF, Chiang HC, Yang MY, Kao ES, Ho YC, Wang CJ . Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits.[J] Agric Food Chem. 2003; 51(18):5472-5477.
doi: 10.1021/jf030065w
[23] Olatunji LA, Adebayo J, Oguntoye OB, Olatunde N, Olatunji VA, Soladoye A . Effects of aqueous extracts of petals of red and green Hibiscus sabdariffa on plasma lipid and hematological variables in rats. Pharm Bio. 2005; 43(5):471-474.
doi: 10.1080/13880200590963934
[24] Adegunloye BJ, Omoniyi JO, Owolabi OA, Ajagbonna OP, Sofola OA, Coker HAB . Petals extract of Hibiscus sabdariffa elicits relaxation of rat aorta by stabilizing the membrane and stimulating Na+-K+-ATPase. Nig[J] Physiol Sci. 1993; 9:74-79.
[25] Olatunji LA, Adebayo JO, Adesokan AA, Olatunji VA, Soladoye AO . Chronic administration of aqueous extract of Hibiscus sabdariffa enhances Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of rat heart. Pharm Biol. 2006; 44(3):213-216
doi: 10.1080/13880200600686129
[26] Horisberger JD, Lemas V, Kraehenbühl JP, Rossier BC . Structure-function relationship of Na,K-ATPase[J]. Annu Rev Physiol, 1991, 53565-584
[27] Wilson PD, Sherwood AC, Palla K, Du J, Watson R, Norman JT . Reversed polarity of Na+-K+-ATPase: mislocation to apical plasma membranes in polycystic kidney disease epithelia[J]. Am J Physiol, 1991,260(3 Pt 2):F420-F430
[28] Zhang Y, Norian JM, Magyar CE, Holstein-Rathlou NH, Mircheff AK , McDonough AA .In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition[J]. Am J Physiol, 1999,276(5 Pt 2):F711-F719
doi: 10.1152/ajpcell.1999.276.3.C711
[29] M’Buyamba-kabanga J, Mbala M, Lijnen P . Alteration of cation metabolism: a prototype etiological model of hypertension[J]. Hypertens Res, 1994,17(3):149-155
doi: 10.1291/hypres.17.149
[30] Blaustein MP . Endogenous ouabain: role in the pathogenesis of hypertension[J]. Kidney Int, 1996,49(6):1748-1753
doi: 10.1038/ki.1996.260
[31] Hussain T, Lokhandwala MF . Renal dopamine receptors and hypertension[J]. Exp Biol Med (Maywood), 2003,228(2):134-142
doi: 10.1177/153537020322800202
[32] Wilson PD . Epithelial cell polarity and disease[J]. Am J Physiol, 1997,272(4 Pt 2):F434-F442
[33] Wilson PD, Devuyst O, Li X, Gatti L, Falkenstein D, Robinson S, Fambrough D, Burrow CR . Apical plasma membrane mispolarization of NaK-ATPase in polycystic kidney disease epithelia is associated with aberrant expression of the β2 isoform[J]. Am J Pathol, 2000,156(1):253-268
doi: 10.1016/S0002-9440(10)64726-8
[34] Steenbergen C, Murphy E, Watts JA, London RE . Correlation between cytosolic free calcium, contracture, ATP, and irreversible ischemic injury in perfused rat heart[J]. Circ Res, 1990,66(1):135-146
doi: 10.1161/01.RES.66.1.135
[35] Adebayo JO, Yakubu MT, Egwim EC, Owoyele VB, Enaibe BU . Effect of ethanolic extract of Khaya senegalensis on some biochemical parameters of rat kidney[J]. J Ethnopharmacol, 2003,88(1):69-72
doi: 10.1016/S0378-8741(03)00193-4
[36] Okolie NP, Uanseoje OS . Enhancement of vascular contractility and inhibition of Na+-K+-ATPase by chronic cyanide intoxication in rabbits[J]. Afr J Sci Technol, 2000, 148-51
[37] Kingsley GR . Procedure for serum protein determinations with a triphosphate biuret reagent[J]. Stand Methods Clin Chem, 1972,7199
[38] Bartholomew RJ, Delaney AM . Sulphonophthaleins as specific reagents for albumin: determination of albumin in serum[J]. Proc Aust Assoc Clin Biochem, 1966, 1214-218
[39] Kirdpon S , Nakorn SN, Kirdpon W . Changes in urinary chemical composition in healthy volunteers after consuming roselle (^Hibiscus sabdariffa Linn. ) juice[J]. J Med Assoc Thai, 1994,77(6):314-321
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[3] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[4] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[5] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[6] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[7] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[8] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[9] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[10] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[11] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[12] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[13] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[14] Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.
[15] Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]