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Journal of Integrative Medicine ›› 2016, Vol. 14 ›› Issue (5): 400-408.doi: 10.1016/S2095-4964(16)60271-6

• Research Article • Previous Articles    

Aphrodisiac activity of aqueous extract of Anthonotha macrophylla P. Beauv. leaves in female Wistar rats

Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye   

  1. Phytomedicine, Toxicology and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, University of Ilorin, Ilorin 24003, Nigeria
  • Received:2016-04-28 Accepted:2016-05-30 Online:2016-09-15 Published:2016-09-15
  • Contact: Prof. Musa Toyin Yakubu; E-mail: tomuyak@gmail.com

Objective

This study investigated the aphrodisiac activity of aqueous extract of Anthonotha macrophylla leaves (AEAML) in female Wistar rats.

Methods

Thirty female rats were assigned into five groups of six animals each, such that rats in groups A, B, C, D and E received orally 1 mL of distilled water only, 5.7 mg/kg body weight (BW) of Exus Ginseng (a polyherbal formulation), 25, 50 and 100 mg/kg BW of AEAML, respectively. After 1 h of treatment, female sexual behaviour parameters were monitored for 30 min. Luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E) and testosterone were also determined using standard methods.

Results

Six secondary metabolites were detected in AEAML with alkaloids (13.00 mg/L) being the highest. AEAML at 25 and 50 mg/kg BW significantly (P<0.05) increased the darting frequency, hopping frequency, lordosis frequency, anogenital grooming, genital grooming and licking behaviour, whereas the darting latency, hopping latency and lordosis latency decreased (P<0.05) in a manner comparable with Exus Ginseng. The 25 and 50 mg/kg BW of AEAML increased (P<0.05) the serum concentrations of FSH, LH and E, like Exus Ginseng, whereas testosterone content was not altered (P>0.05). The 100 mg/kg BW of AEAML did not alter (P>0.05) the sexual behaviour parameters, serum FSH, LH and E contents, whereas testosterone content increased significantly. The ovarian histology of the animals treated with Exus Ginseng, AEAML at 25 and 50 mg/kg BW revealed developing and ruptured follicles with numerous corpora lutea in the stroma, whereas the 100 mg/kg BW of AEAML produced fewer follicles and corpora lutea.

Conclusion

The enhanced proceptive, receptive and orientation components of the female sexual behaviour by the 25 and 50 mg/kg BW of AEAML confers sexual invigorating potential on the plant. This study thus justifies the folkloric claim of the plant as a female aphrodisiac.

Key words: Plant extracts, Anthonotha macrophylla, Leguminosae, Aphrodisiacs, Estradiol, Sexual behaviour

1 González M, Viáfara G, Caba F, Molina T, Ortiz C.Libido and orgasm in middle-aged woman.Maturitas. 2006; 53(1): 1-10.
2 Walton B, Thorton T.Female sexual dysfunction.Curr Womens Health Rep. 2003; 3(4): 319-326.
3 Modelska K, Milián M.Treatment of female sexual dysfunction in postmenopausal women: what is the evidence? Rev Gynaecol Pract. 2004; 4(2): 121-132.
4 Fajewonyomi BA, Orji EO, Adeyemo AO.Sexual dysfunction among female of reproductive age in a hospital setting in Nigeria.J Health Popul Nutr. 2007; 25(1): 101-106.
5 Goldstein I.Female sexual arousal disorder: new insights.Int J Impot Res. 2000; 12(Suppl 4): S152-S157.
6 Bermen JR, Adhiara SP, Goldstein I.Anatomy and physiology of female sexual function and dysfunction: classification, evaluation and treatment options.Eur Urol. 2000; 38(1): 20-29.
7 Lawal IO, Uzokwe V, Igboanugo ABI, Adio AF, Awosan EA, Nwogwugwu JO, Faloye B, Olatunji BP, Adesoga AA.Ethnomedicinal information on collation and identification of some medicinal plants in Research Institutes of South-west Nigeria. Afr J Pharm Pharmacol.2010; 4(1): 1-7.
8 Burkill HM.The useful plants of west tropical Africa. Kew: Royal Botanical Garden. 2004.
9 Keay RWJ, Onochie CFA, Stanfield DP.Trees of Nigeria. Lagos: Nigerian National Press Limited. 1964.
10 Guédé NZ, N’guessan K, Dibié TE, Gvellier P.Ethnopharmacological study of plants used to treat malaria, in traditional medicine by Bete populations of Issia (Côte d’Ivorie). J Pharm Sci Res.2010; 2(4): 216-227.
11 Durunna CS.Aspect of biochemical composition ofAnthonotha macrophylla seeds. Animal Prod Res Adv. 2006; 2(4): 217-220.
12 Ugoeze KC, Ehianeta T, Alaribe C, Anyakora C.Analysis and identification of oils from seed extract ofAnthonotha macrophylla using gas chromatography-mass spectrometry (GC-MS). Afr J Biotechnol. 2014; 13(22): 2260-2264.
13 Durunna CS, Nwabueze UN, Afulike CE, Ezeokeke CT.Value ofAnthonotha macrophylla leaf meal as feed ingredient on layer performance and egg quality. Int J Trop Agric Food Syst. 2007; 1(1): 94-96.
14 Trease GE, Evans WC.A textbook of pharmacognosy. London: Bailliere-Tindall Ltd. 1983: 343-383.
15 Kokate KC.Practical pharmacognosy. 4th ed. New Delhi: Vallabh Prakashan. 1997: 218.
16 Sofowora A.Medicinal plants and traditional medicine in Africa. Ibadan: Spectrum Books Limited. 1993: 134-156.
17 Hudson BJF, El-Difrawi EA.The sapogenins of the seeds of four Lupin species.J Plant Foods. 1979; 3(1): 181-186.
18 Ying C, Wan D.Quantitative determination of total and individual flavonoids in stems and leaves ofBuddleja davidii and Buddleja albiflora. Pharmacogn Mag. 2012; 8(32): 273-279.
19 Solich P, Sedliakova V, Karlíček R.Spectrophotometric determination of cardiac glycosides by flow-injection analysis.Anal Chim Acta. 1992; 269(2): 199-203.
20 Ragazzi E, Veronese G.Quantitative analysis of phenolics compounds after thin layer chromatographic separation.J Chromatogr. 1973; 77(2): 369-375.
21 Edeoga HO, Okwu DE, Mbaebie BO.Phytochemical constituents of some Nigeria medicinal plants.Afr J Biotechnol. 2005; 4(7): 685-688.
22 Harborne AJ.Phytochemical methods: a guide to modern techniques of plant analysis. 2nd ed. London: Chapman and Hall. 1998: 54-84.
23 Giuliano F, Rampin O, McKenna K. Animal models used in the study of erectile dysfunction. In: Carson C, Kirby R, Goldstein I. Textbook of erectile dysfunction. Oxford: Isis Medical Media. 1999: 43-50.
24 Yakubu MT, Akanji MA, Oladiji AT.Aphrodisiac potentials of the aqueous extract ofFadogia agrestis(Schweinf. Ex Hiern) stem in male albino rats. Asian J Androl. 2005; 7(4): 399-404.
25 Krause WJ.The art of examining and interpreting histologic preparations: a student handbook. Pearl River, New York: The Parthenon Publishing Group Inc. 2001: 9-10.
26 Drury RAB, Wallington EA.Carleton’s histological techniques. Oxford: Oxford University Press. 1980: 258-276.
27 da Silva CV, Borges FM, Velozo ES. Phytochemistry of some Brazilian plants with aphrodisiac activity. In: Rao V. Phytochemicals—a global perspective of their role in nutrition and health. Croatia: InTech. 2012: 953-978.
28 Yang DJ, Lu TJ, Hwang LS.Isolation and identification of steroidal saponins in Taiwanese yam cultivar(Dioscorea pseudojaponica Yamamoto). J Agric Food Chem.2003; 51(22): 6438-6444.
29 Yakubu MT, Akanji MA, Oladiji AT, Olatinwo AO, Adesokan AA, Yakubu MO, Owoyele BV, Sunmonu TO, Ajao MS.Effect ofCnidoscolous aconitifolius(Miller) I.M. Johnston leaf extract on reproductive hormones of female rats. Iran J Rep Med. 2008; 6(3): 149-155.
30 Martino-Andrade AJ, Morais RN, Spercoski KM, Rossi SC, Vechi MF, Golin M, Lombardi NF, Greca CS, Dalsenter PR.Effects ofTribulus terrestris on endocrine sensitive organs in male and female Wistar rats. J Ethnopharmacol. 2010; 127(1): 165-170.
31 Ng PC, Ho DD, Ng KH, Kong YC, Cheng KF, Stone G.Mixed estrogenic and anti-estrogenic activities of yuehchukere-a bis-indole alkaloid. Eur J Pharmacol.1994; 264(1): 1-12.
32 Abedi A, Parviz M, Karimian SM, Rodsari HRS.Aphrodisiac activity of aqueous extract ofPhoenix dactylifera pollen in male rats. Adv Sex Med. 2012; 3: 28-34.
33 Aslam M, Sial AA.Effect of hydroalcoholic extract ofCydonia oblonga Miller (Quince) on sexual behavior of Wistar rats. Adv Pharmacological Sci. 2014; 2014(2014): 1-6.
34 Peluso MR.Flavonoids attenuate cardiovascular disease, inhibit phosphodiesterase, and modulate lipid homeostasis in adipose tissue and liver.Exp Biol Med (Maywood). 2006; 231(8): 1287-1299.
35 Aswar UM, Bhaskaran S, Mohan V, Bodhankar SL.Estrogenic activity of friedelin rich fraction (IND-HE) separated fromCissus quadrangularis and its effect on female sexual function. Pharmacognosy Res. 2010; 138-145.
36 Olivier B, Chan JS, Snoeren EM, Olivier JD, Veening JG, Vinkers CH, Waldinger MD, Oosting RS.Differences in sexual behavior in male and female rodents: role of serotonin.Curr Top Behav Neurosci. 2011; 8: 15-36.
37 Moundipa FP, Kamtchouing P, Koueta N, Tantchou J,Foyang NP, Mbiapo FT.Effects of aqueous extracts ofHibiscus macranthus and Basella alba in mature rat testis function. J Ethnopharmacol. 1999; 65(2): 133-139.
38 Spiteri T, Musatov S, Ogawa S, Ribeiro A, Pfaff DW, Agmo A.The role of oestrogen receptor alpha in the medial amygdala and ventromedial nucleus of the hypothalamus in social recognition, anxiety and aggression.Behav Brain Res. 2010; 210(2): 211-220.
39 Yakubu MT, Afolayan AJ.Reproductive toxicologic evaluations ofBulbine natalensis Baker stem extract in albino rats. Theriogenology. 2009; 72(3): 322-332.
40 Krishnamoorthy P, Sivaaranjani K, Rajeswari K,Kalaiselvan D.Effect of Andrographis paniculata Wall. Ex Nees root extract fractions on estrogen, FSH, LH, progesterone and ovary of female albino rats, Rattus norvegicus.Indian J Nat Prod Resour. 2013; 4(1): 42-47.
41 Oyesola TO, Oyesola OA, Okoye CS.Effect of aqueous extract ofAspilia africana on reproductive functions offemale Wistar rats. Pak J Biol Sci. 2010; 13(3): 126-131.
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