Search JIM Advanced Search

Journal of Integrative Medicine ›› 2015, Vol. 13 ›› Issue (3): 185-193.doi: 10.1016/S2095-4964(15)60175-3

• Research Article • Previous Articles     Next Articles

Antioxidant, antiangiogenic and antiproliferative activities of root methanol extract of Calliandra portoricensis in human prostate cancer cells

Oluwatosin Adaramoyea,b, Bettina Erguena, Olubukola Oyebodec, Bianca Nitzsched, Michael Höpfnerd, Klaus Junga,e, Anja Rabiena,e#br#   

  1. a Department of Urology, University Hospital Charite, 10117 Berlin, Germany
    b Drug Metabolism and Toxicology Section, Department of Biochemistry, University of Ibadan, Ibadan 20005, Nigeria
    c Membrane Biochemistry and Biophysics Section, Department of Biochemistry, University of Ibadan, Ibadan 20005, Nigeria
    d Institute of Physiology, University Hospital Charite, 10117 Berlin, Germany
    e Berlin Institute for Urologic Research, 10117 Berlin, Germany
  • Received:2014-10-20 Accepted:2015-02-11 Online:2015-05-10 Published:2015-05-15

Prostate cancer (PCa) is a major health concern. Calliandra portoricensis (CP) is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated.
Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl (DPPH) and hydroxyl (OH?) radicals-scavenging methods. PC-3 and LNCaP (androgen-refractory and androgen-dependent PCa-derived cell lines) were cultured and treated with CP (10, 50 and 100 μg/mL). Effects of CP on cells were determined by cytotoxicity assay (lactate dehydrogenase, LDH) and viability assay (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate, XTT). DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane (CAM) assay.
CP showed significant scavenging of DPPH and OH- radicals. CP significantly (P < 0.05) inhibited lipid peroxidation in a dose-dependent manner. Precisely, CP (10, 50 and 100 μg/mL) inhibited PC-3 and LNCaP growth by 7%, 74% and 92%, and 27%, 73%, and 85% respectively at 48 h. CP had low toxicity in vitro at its half inhibitory concentration dose. Detection of cell death induced by CP at 50 μg/mL showed higher enrichment factors in LNCaP (7.38 ± 0.95) than PC-3 (3.48 ± 0.55). Also, treatment with CP (50 μg/mL) significantly reduced network of vessels in CAM, suggesting its antiangiogenic potential.


Calliandra portoricensis elicited antioxidant, antiangiogenic and antiproliferative effects in PCa cells.

Key words: Calliandra portoricensis, Plant extracts, Antiproliferation, Antiangiogenesis, Prostatic neoplasms

[1] Parkin DM, Ferlay J, Curado MP, Bray F, Edwards B, Shin HR, Forman D . GLOBOCAN 2012 v1.0, Cancer incidence and mortality worldwide: IARC CancerBase No. 11. Lyon, France: International Agency for Research on Cancer. 2013.
[2] Thangam R, Suresh V, Rajkumar M, Vincent JD, Gunasekaran P, Anbazhagan C, Kaveri K, Kannan S . Antioxidant and in vitro anticancer effect of 2-pyrrolidinone rich fraction of Brassica oleracea var. capitata through induction of apoptosis in human cancer cells. Phytother Res, 2013,27(11):1664-1670
doi: 10.1002/ptr.v27.11
[3] Matić IZ, Aljancić I, Vajs V, Jadranin M, Gligorijević N, Milosavljević S, Juranić ZD . Cancer-suppressive potential of extracts of endemic plant Helichrysum zivojinii: effects on cell migration, invasion and angiogenesis. Nat Prod Commun, 2013,8(9):1291-1296
[4] Hwang YJ, Lee EJ, Kim HR, Hwang KA . In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var. lilacina. BMC Complement Altern Med, 2013,13:310
doi: 10.1186/1472-6882-13-310
[5] Armania N, Yazan LS, Ismail IS, Foo JB, Tor YS, Ishak N, Ismail N, Ismail M . Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines(MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis. Molecules, 2013,18(11):13320-13339
doi: 10.3390/molecules181113320
[6] Akah PA, Nwaiwu JI . Anticonvulsant activity of root and stem extracts of Calliandra portoricensis. J Ethnopharmacol, 1988,22(2):205-210
doi: 10.1016/0378-8741(88)90128-6 pmid: 3374152
[7] Aguwa CN, Lawal AM . Pharmacologic studies on the active principles of Calliandra portoticensis leaf extracts. J Ethnopharmacol, 1988,22(1):63-71
doi: 10.1016/0378-8741(88)90231-0 pmid: 3352286
[8] Agunu A, Abdurahman EM, Shok M, Yusuf SA . Analgesic activity of the roots and leaves extracts of Calliandra portoricensis. Fitoterapia, 2005,76(5):442-445
doi: 10.1016/j.fitote.2005.03.008 pmid: 15905046
[9] Irvine FR. Woody plants of Ghana with special reference to their uses[M]. London: Oxford University Press, 1961,: 13-25
[10] Oyaizu M . Studies on the product of browning reaction prepared from glucose amine. Jpn J Nutr, 1986,44(3):307-315
doi: 10.5264/eiyogakuzashi.44.307
[11] Singleton VL, Orthofer R, Lamuela-Raventos RM . Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Cioalteau reagent. Methods Enzymol, 1999,299(1):152-178
doi: 10.1016/S0076-6879(99)99017-1
[12] Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG . Determination of the total phenolic, flavonoid and proline contents in Burkina Faso honey, as well as their radical scavenging activity. Food Chem, 2005,91(2):571-577
doi: 10.1016/j.foodchem.2004.10.006
[13] Ohkawa H, Ohishi N, Yagi K . Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 1979,95(2):351-358
doi: 10.1016/0003-2697(79)90738-3
[14] Halliwell B . Oxidants and human disease: some new concepts. FASEB J, 1987,1(5):358-364
doi: 10.1096/fasebj.1.5.2824268 pmid: 2824268
[15] Mensor LL, Menezes FS , Leit?o GG, Reis AS, dos Santos TC, Coube CS, Leit?o SG.Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res, 2001,15(2):127-130
doi: 10.1002/ptr.v15:2
[16] Rodrigues J, Abramjuk C, Vásquez L, Gamboa N, Domínguez J, Nitzsche B, Höpfner M, Georgieva R, B?umler H, Stephan C, Jung K, Lein M, Rabien A. New 4-maleamic acid and 4-maleamide peptidyl chalcones as potential multitarget drugs for human prostate cancer. Pharm Res, 2011,28(4):907-919
doi: 10.1007/s11095-010-0347-8
[17] Ruiz Y, Rodrígues J, Arvelo F, Usubillaga A, Monsalve M, Diez N, Galindo-Castro I . Cytotoxic and apoptosis-inducing effect of ent-15-oxo-kaur-16-en-19-oic acid, a derivative of grandiflorolic acid from Espeletia schultzii. Phytochemistry, 2008,69(2):432-438
doi: 10.1016/j.phytochem.2007.07.025
[18] Gerlier D, Thomasset N . Use of MTT colorimetric assay to measure cell activation. J Immunol Methods, 1986,94(1-2):57-63
doi: 10.1016/0022-1759(86)90215-2 pmid: 3782817
[19] Nitzsche B, Gloesenkamp C, Schrader M, Ocker M, Preissner R, Lein M, Zakrzewicz A, Hoffmann B , H?pfner M.Novel compounds with antiangiogenic and antiproliferative potency for growth control of testicular germ cell tumours. Br J Cancer, 2010,103(1):18-28
doi: 10.1038/sj.bjc.6605725
[20] Fotsis T, Pepper MS, Aktas E, Breit S, Rasku S, Adlercreutz H ,Wähälä K, Montesano R, Schweigerer L. Flavonoid, dietary-derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Res, 1997,57(14):2916-2921
pmid: 9230201
[21] Wagner H, Geyer B, Kiso Y, Hikino H, Rao GS . Coumestans as the main active principles of the liver drugs Eclipta alba and Wedelia calendulacea. Planta Med, 1986,( 5):370-372
[22] Andrade VS, Rojas DB, Oliveira L , Nunes ML, de Castro FL, Garcia C, Gemelli T, de Andrade RB, Wannmacher CM. Creatine and pyruvate prevent behavioral and oxidative stress alterations caused by hypertryptophanemia in rats. Mol Cell Biochem, 2012,362(1-2):225-232
doi: 10.1007/s11010-011-1147-0
[23] Adeniji A, Odukoya O . In vitro lipid peroxidation as progression of liver injury at the cellular level. Planta Med, 2010,76:307
doi: 10.1055/s-0030-1264605
[24] Wang G, Reed E, Li QQ . Apoptosis in prostate cancer: progressive and therapeutic implications. Int J Mol Med, 2004,14(1):23-24
doi: 10.3892/ijmm.14.1.23 pmid: 15202013
[25] Kerr JF, Winterford CM, Harmon BV . Apoptosis: its significance in cancer and cancer therapy. Cancer, 1994,73(8):2013-2026
doi: 10.1002/(ISSN)1097-0142
[26] Hanahan D, Weinberg RA . The hallmarks of cancer. Cell, 2000,100(1):57-70
doi: 10.1016/S0092-8674(00)81683-9
[27] Tafrihi M, Toosi S, Minaei T, Gohari AR, Niknam V, Arab Najafi SM . Anticancer properties of Teucrium persicum in PC-3 prostate cancer cells. Asian Pac J Cancer Prev, 2014,15(2):785-791
doi: 10.7314/APJCP.2014.15.2.785 pmid: 24568496
[28] Pepper MS . Manipulating angiogenesis. From basic science to the bedside. Arterioscler Thromb Vasc Biol, 1997,17(4):605-619
doi: 10.1161/01.ATV.17.4.605
[29] Lin CM, Chen YH, Ong JR, Ma HP, Shyu KG, Bai KJ . Functional role of wogonin in anti-angiogenesis. Am J Chin Med, 2012,40(2):415-427
doi: 10.1142/S0192415X12500322 pmid: 22419433
[30] Wang T, Liu J, Xiao XQ . Cantharidin inhibits angiogenesis by suppressing VEGF-induced JAK1/STAT3, ERK and AKT signaling pathways. Arch Pharm Res, 2015,38(2):282-289
doi: 10.1007/s12272-014-0383-8
[1] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[2] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[3] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[4] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[5] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Thongchai Koobkokkruad, Chutichot Pattamadilok, Nontima Vardhanabhuti . Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2018, 16(6): 427-434.
[6] Shohini Chakraborty, Nashra Afaq, Neelam Singh, Sukanta Majumdar. Antimicrobial activity of Cannabis sativaThuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of Integrative Medicine, 2018, 16(5): 350-357.
[7] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[8] Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye. Aphrodisiac activity of aqueous extract of Anthonotha macrophylla P. Beauv. leaves in female Wistar rats. Journal of Integrative Medicine, 2016, 14(5): 400-408.
[9] Nisha Panth, Keshav Raj Paudel, Rajendra Karki. Phytochemical profile and biological activity of Juglans regia. Journal of Integrative Medicine, 2016, 14(5): 359-373.
[10] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[11] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[12] Wei Chiang Chan Eric, Kuin Wong Siu. Phytochemistry and pharmacology of ornamental gingers, Hedychium coronarium and Alpinia purpurata: A review. Journal of Integrative Medicine, 2015, 13(6): 368-379.
[13] Rashmi Singh, Shatruhan Sharma, Veena Sharma. Comparative and quantitative analysis of antioxidant and scavenging potential of Indigofera tinctoria Linn. extracts. Journal of Integrative Medicine, 2015, 13(4): 269-278.
[14] Swapnil P. Borse, Bhagyashree B. Kamble. Effects of Ayurvedic Rasayana botanicals on CYP3A4 isoenzyme system. Journal of Integrative Medicine, 2015, 13(3): 165-172.
[15] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
Full text



[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239