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Journal of Integrative Medicine ›› 2015, Vol. 13 ›› Issue (3): 185-193.doi: 10.1016/S2095-4964(15)60175-3

• Research Article • Previous Articles     Next Articles

Antioxidant, antiangiogenic and antiproliferative activities of root methanol extract of Calliandra portoricensis in human prostate cancer cells

Oluwatosin Adaramoyea,b, Bettina Erguena, Olubukola Oyebodec, Bianca Nitzsched, Michael Höpfnerd, Klaus Junga,e, Anja Rabiena,e#br#   

  1. a Department of Urology, University Hospital Charite, 10117 Berlin, Germany
    b Drug Metabolism and Toxicology Section, Department of Biochemistry, University of Ibadan, Ibadan 20005, Nigeria
    c Membrane Biochemistry and Biophysics Section, Department of Biochemistry, University of Ibadan, Ibadan 20005, Nigeria
    d Institute of Physiology, University Hospital Charite, 10117 Berlin, Germany
    e Berlin Institute for Urologic Research, 10117 Berlin, Germany
  • Received:2014-10-20 Accepted:2015-02-11 Online:2015-05-10 Published:2015-05-15

Objective
Prostate cancer (PCa) is a major health concern. Calliandra portoricensis (CP) is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated.
Methods
Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl (DPPH) and hydroxyl (OH?) radicals-scavenging methods. PC-3 and LNCaP (androgen-refractory and androgen-dependent PCa-derived cell lines) were cultured and treated with CP (10, 50 and 100 μg/mL). Effects of CP on cells were determined by cytotoxicity assay (lactate dehydrogenase, LDH) and viability assay (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate, XTT). DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane (CAM) assay.
Results
CP showed significant scavenging of DPPH and OH- radicals. CP significantly (P < 0.05) inhibited lipid peroxidation in a dose-dependent manner. Precisely, CP (10, 50 and 100 μg/mL) inhibited PC-3 and LNCaP growth by 7%, 74% and 92%, and 27%, 73%, and 85% respectively at 48 h. CP had low toxicity in vitro at its half inhibitory concentration dose. Detection of cell death induced by CP at 50 μg/mL showed higher enrichment factors in LNCaP (7.38 ± 0.95) than PC-3 (3.48 ± 0.55). Also, treatment with CP (50 μg/mL) significantly reduced network of vessels in CAM, suggesting its antiangiogenic potential.

Conclusion

Calliandra portoricensis elicited antioxidant, antiangiogenic and antiproliferative effects in PCa cells.

Key words: Calliandra portoricensis, Plant extracts, Antiproliferation, Antiangiogenesis, Prostatic neoplasms

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