Search JIM Advanced Search

Journal of Integrative Medicine ›› 2018, Vol. 16 ›› Issue (4): 290-296.doi: 10.1016/j.joim.2018.05.003

• Original Experimental Research • Previous Articles    

Preliminary optimization of a Chinese herbal medicine formula based on the neuroprotective effects in a rat model of rotenone-induced Parkinson’s disease

Xu-xia Bao a, b, Hui-han Ma a, b, Hao Ding a, b, Wen-wei Li a, b, Min Zhu c   

  1. a Department of Integrative Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
    b Institute of Neurology, Institutes of Integrative Medicine of Fudan University, Shanghai 200032, China
    c Shanghai Key Laboratory of Visual Impairment and Restoration, Eye & ENT Hospital, Fudan University, Shanghai 200030, China
  • Received:2017-12-10 Accepted:2018-04-15 Online:2018-07-09 Published:2018-05-22
  • Contact: Wen-wei Li; E-mail: li.wenwei@zs-hospital.sh.cn Min Zhu; E-mail: minzhu@fdeent.org

Objective
The main objective of this study was to preliminarily determine the optimum formulation of a Chinese herbal formula that may have neuroprotective effects against rotenone-induced Parkinson’s disease (PD).


Methods

Seven recipes were made from Dihuang (DH, Rehmannia glutinosa Libosch), Roucongrong (RCR, Cistanche deserticolaY.C.Ma), Niuxi (NX, Achyranthes bidentata Bl.) and Shanzhuyu (SZY, Cornus officinalis Sieb. et Zucc) in different proportions, according to the principles of uniform design (4 factors 7 levels). Tyrosine hydroxylase (TH)-positive neurons in substantia nigra pars compacta (SNpc) were detected by immunohistochemistry and rotenone-exposure days necessary to induce PD symptoms were recorded. To probe one likely mechanism of the formulas, echinacoside (ECH) concentrations of all seven recipes were determined by high-performance liquid chromatography and related to number of TH-positive neurons.


Results

The data showed that recipe 4 (DH:RCR:SZY:NX?=?1:1:1:1) and recipe 7 (DH:RCR:SZY:NX?=?7:5:3:1) partially reversed rotenone-induced death of TH-positive neurons in the SNpc and significantly increased rotenone-exposed days compared with model group. Pharmacologically, there was not a strong correlation between ECH concentration and TH-positive neurons.


Conclusion

The investigated formulations of Chinese herbs had neuroprotective effects against PD models, and the neuroprotective effects were weakly related to the proportion of key herbs. However the neuroprotective effects of the formula may not result from a single active constituent.

Key words: Parkinson’s disease, Rotenone, Chinese herb, Echinacoside, Uniform design

[1] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[2] Goulnar Kasymjanova, Anh-Thi Tran, Victor Cohen, Carmela Pepe, Lama Sakr, David Small, Jason Scott Agulnik, Robert Thomas Jagoe. The use of a standardized Chinese herbal formula in patients with advanced lung cancer: A feasibility study. Journal of Integrative Medicine, 2018, 16(6): 390-395.
[3] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[4] Liliana Zazueta-Beltrán, Lorena Medina-Aymerich, Nadia Estela Díaz-Triste, Aracely Evangelina Chávez-Piña, Gilberto Castañeda-Hernández, Leticia Cruz-Antonio. Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats. Journal of Integrative Medicine, 2017, 15(2): 151-157.
[5] Tom Fleischer, Tung-Ti Chang, Hung-Rong Yen. Post-hematopoietic stem cell transplantation in patients with hematologic disorders: Chinese herbal medicine for an unmet need. Journal of Integrative Medicine, 2016, 14(5): 322-335.
[6] JiaXia, Li-qunHe, XiaoSu. Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Journal of Integrative Medicine, 2016, 14(3): 165-173.
[7] Xiao-ping Huang, Huang Ding, Jin-dong Lu, Ying-hong Tang, Bing-xiang Deng, Chang-qing Deng . Autophagy in cerebral ischemia and the effects of traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(5): 289-296.
[8] Swapnil P. Borse, Bhagyashree B. Kamble. Effects of Ayurvedic Rasayana botanicals on CYP3A4 isoenzyme system. Journal of Integrative Medicine, 2015, 13(3): 165-172.
[9] Chang-qing Zhao, Yang Zhou, Jian Ping, Lie-ming Xu. Traditional Chinese medicine for treatment of liver diseases: Progress, challenges and opportunities. Journal of Integrative Medicine, 2014, 12(5): 401-408.
[10] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[11] Yan-bin Shi, Hui-li Li, Hai-qin Wang, Yan-biao Yang, Xiao-yun Zhang, Hui Wang, Zong-jie Zhu, Zhi-ye Zhang, Cheng-an Zhang. Simultaneous determination of five anthraquinones in a Chinese traditional preparation by RP-HPLC using an improved extraction procedure. Journal of Integrative Medicine, 2014, 12(5): 455-462.
[12] Zhong-liang Liu, Wei-rong Zhu, Wen-chao Zhou, Hai-feng Ying, Lan Zheng, Yuan-biao Guo, Jing-xian Chen, Xiao-heng Shen. Traditional Chinese medicinal herbs combined with epidermal growth factor receptor tyrosine kinase inhibitor for advanced non-small cell lung cancer: A systematic review and meta-analysis. Journal of Integrative Medicine, 2014, 12(4): 346-358.
[13] Zemin Yao​, Li Zhang​, Guang Ji​. Efficacy of polyphenolic ingredients of Chinese herbs in treating dyslipidemia of metabolic syndromes. Journal of Integrative Medicine, 2014, 12(3): 135-146.
[14] Jin Rui, Lin Zhi-jian, Xue Chun-miao, Zhang Bing. An improved association-mining research for exploring Chinese herbal property theory: Based on data of the Shennong’s Classic of Materia Medica. Journal of Integrative Medicine, 2013, 11(5): 352-365.
[15] Jie Zhang, Yun-zhi Ma, Xiao-ming Shen. Evaluation on the efficacy and safety of Chinese herbal medication Xifeng Dingchan Pill in treating Parkinson’s disease: Study protocol of a multicenter, open-label, randomized active-controlled trial. Journal of Integrative Medicine, 2013, 11(4): 285-290.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[4] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[5] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[6] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[7] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[8] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[9] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[10] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035