Preliminary optimization of a Chinese herbal medicine formula based on the neuroprotective effects in a rat model of rotenone-induced Parkinson’s disease

doi:10.1016/j.joim.2018.05.003

Abstract

Objective
The main objective of this study was to preliminarily determine the optimum formulation of a Chinese herbal formula that may have neuroprotective effects against rotenone-induced Parkinson’s disease (PD).

Methods

Seven recipes were made from Dihuang (DH, Rehmannia glutinosa Libosch), Roucongrong (RCR, Cistanche deserticolaY.C.Ma), Niuxi (NX, Achyranthes bidentata Bl.) and Shanzhuyu (SZY, Cornus officinalis Sieb. et Zucc) in different proportions, according to the principles of uniform design (4 factors 7 levels). Tyrosine hydroxylase (TH)-positive neurons in substantia nigra pars compacta (SNpc) were detected by immunohistochemistry and rotenone-exposure days necessary to induce PD symptoms were recorded. To probe one likely mechanism of the formulas, echinacoside (ECH) concentrations of all seven recipes were determined by high-performance liquid chromatography and related to number of TH-positive neurons.

Results

The data showed that recipe 4 (DH:RCR:SZY:NX?=?1:1:1:1) and recipe 7 (DH:RCR:SZY:NX?=?7:5:3:1) partially reversed rotenone-induced death of TH-positive neurons in the SNpc and significantly increased rotenone-exposed days compared with model group. Pharmacologically, there was not a strong correlation between ECH concentration and TH-positive neurons.

Conclusion

The investigated formulations of Chinese herbs had neuroprotective effects against PD models, and the neuroprotective effects were weakly related to the proportion of key herbs. However the neuroprotective effects of the formula may not result from a single active constituent.

Key words: Parkinson’s disease, Rotenone, Chinese herb, Echinacoside, Uniform design

Xu-xia Bao, Hui-han Ma, Hao Ding, Wen-wei Li, Min Zhu . Preliminary optimization of a Chinese herbal medicine formula based on the neuroprotective effects in a rat model of rotenone-induced Parkinson’s disease. Journal of Integrative Medicine, 2018, 16(4): 290-296.

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URL: http://www.jcimjournal.com/EN/10.1016/j.joim.2018.05.003

http://www.jcimjournal.com/EN/Y2018/V16/I4/290

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